Advanced searches left 3/3

Zika Virus - Springer Nature

Summarized by Plex Scholar
Last Updated: 09 January 2023

* If you want to update the article please login/register

MEK/ERK activation plays a decisive role in Zika virus morphogenesis and release

In recent years, millions of people have been affected by the high incidence of dengue fever, Zika infection in children, and yellow fever's high mortality have affected millions. We reviewed the role of the MEKV gene and the effects of the MEK inhibitor trametinib on the Asian ZIKV strain PE243 and the prototype African ZIKV strain MR766, which explore genome replication, morphogenesis, and viral release in this report. At 12 and 18 hours postinfection, ZIKV infection-triggered ERK phosphorylation in Vero cells, which led to ERK phosphorylation. Trametinib showed sustained antiviral activity, limiting both ZIKV strains for at least four days, and electron microscopy revealed probable inhibition of ZIKV morphogenesis, as shown by electron microscopy. Trametinib delayed late stages of viral replication, according to 6-hour studies, and the titration of intra- or extracellular virions revealed that the therapy had a major effect on viral morphogenesis and release.

Source link: https://doi.org/10.1007/s00705-022-05632-2


Brain Organoids as a Model to Study Zika Virus and SARS-CoV-2 Infections

Brain organoids are essential in vitro platforms for the study of the effects of viral infections on brain growth and function, as well as potential applications to new emerging viral threats. It was possible to demonstrate that Zika virus infects neural stem cells, disrupting the cell cycle and neurogenesis, leading to microcephaly, a dramatic decline in the brain. Here, we describe a technique for the infection of human brain organoid cultures with Zika and Sars-CoV-2 viruses, which can be used to analyze neuronal phenotypes, change in neuronal function, host-u2013pathogen interactions, and drug testing.

Source link: https://doi.org/10.1007/978-1-0716-2720-4_10


Comparative Study of Drug Efficiency of Semecarpus anacardium and Tridax procumbens Against Zika Virus

If it affects pregnant women, the Zika virus infection is not lethal. The Semecarpus anacardium and Tridax procumbens are well known for their medicinal properties, including antimicrobial and antiviral activities. The S. anacardium and T. procumbens were subjected to Lipinski's rule of five, and molecular docking experiments were performed. u22122. 40 Kcal/mol and n-heneicosane's score of u22122. 40 Kcal/mol were among all of the compounds tested, with benzoic acid,4-ethyl ester's minimum score of u22122. 40 Kcal/mol and n-heneicosane as a result of u22121. 34 Kcal/mol as the first score out of 30 Kcal/mol Considering the findings of the in silico experiment, the comparative analysis of these plants against the viral enzyme shows that the phytocompounds of T. procumbens have active inhibition when compared to the S. anacardium, which can be tested in vitro and in vivo studies.

Source link: https://doi.org/10.1007/978-1-0716-2683-2_13


Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response

Methods The effect of ZIKV infection on mature neurons and microglia was determined on both molecular and cellular levels, in vitro using immunocompetent primary cultured neurons and microglia, and in vivo in adult immunocompetent mice following intracranial ZIKV inoculation. From C57BL/6 and the genetically diverse Collaborative Cross mouse strains, showing a wide variety of vulnerability to ZIKV infection, we've come to question the relationship between the effects of ZIKV infection on neurons and microglia, as well as in vivo susceptibility to ZIKV. Only neurons and not microglial cells were found in vivo and vitro, so in vitro and vitro, raising the question of the mechanisms underlying microglia activation following brain ZIKV infection. After infection triggered non-infected microglial cells, type-I interferons were secretly injected by neurons in a primary cultured microglia, which were characterized by neurons later.

Source link: https://doi.org/10.1186/s12974-022-02668-8

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions