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Zika Virus - PubMed

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Last Updated: 09 January 2023

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Novel Therapeutic Nutrients Molecules That Protect against Zika Virus Infection with a Special Note on Palmitoleate.

Zika virus, a Flaviviridae virus and a single strand RNA virus, is a flavivirus from the Flaviviridae family and a single-strand RNA virus with a single strand RNA virus. ZIKV infections were extremely low insignificant with sporadic outbreaks on the Asian and African continents before 2006. We've also reviewed recent developments relating to the novel therapeutic nutrient molecules that have been shown to have activity against Zika virus infected cells. Also investigate the development of ZIKV-induced endoplasmic reticulum and apoptosis, as well as the protective role of palmitoleate against ZIKV-induced ER stress and apoptosis in the placental trophoblasts.

Source link: https://doi.org/10.3390/nu15010124


American-Asian- and African lineages of Zika virus induce differential pro-inflammatory and Interleukin 27-dependent antiviral responses in human monocytes.

Monocytes are the earliest blood cell type to be infected by ZIKV, and they have been shown to be linked to ZIKV pathogenesis. The first ZIKV epidemic was recorded in Africa and Asia, but it is unknown if African- and Asian-lineages of ZIKV have different effects on host immune response. The ZIKV Puerto Rico strain, according to the transcriptomic review, promotes a higher pro-inflammatory response via TLR2 signaling and NF-kB activation, resulting in a greater IL27-dependent antiviral activity than the ZIKV N. . . . ia strain. Human monocytes are more susceptible to ZIKV infection in Colombia than ZIKV from Dakar, in addition. In addition, we show that treating monocytes with IL27 results in reduced release of ZIKV particles in a dose-dependent manner, with an EC50 =10. 23 ng/mL to ZIKV from Dakar.

Source link: https://doi.org/10.1016/j.virusres.2023.199040


MEK/ERK activation plays a decisive role in Zika virus morphogenesis and release.

We reviewed the role of the MEKV pathway and the effects of the MEK inhibitor trametinib on the Asian ZIKV strain PE243 and the prototype African ZIKV strain MR766, looking at genome replication, morphogenesis, and viral release in this report. At 12 and 18 hours postinfection, ZIKV infection provoked ERK phosphorylation in Vero cells. Trametinib showed persistent antiviral activity, inhibiting both ZIKV strains for at least four days, and electron microscopy showed probable inhibition of ZIKV morphogenesis. ZIKV PE243 can complete one cycle in Vero cells in 14 hours, with genome replication established around 8 hpi, intracellular viral particles at 12 hpi, and extracellular progeny at 14 hpi. Thus, ZIKV stimulated ERK phosphorylation during viral propagation and release, which was linked to trametinib blocking both the signaling pathway and viral replication.

Source link: https://doi.org/10.1007/s00705-022-05632-2


Nuclear accumulation of host transcripts during Zika Virus Infection.

In utero, Zika virus infects fetal neural progenitor cells, causing severe neurodevelopmental abnormalities. Infected NPCs, the pathways involved in normal brain development are dysfunctional, but how ZIKV centrally reprograms pathways remains unclear. We find that a subset of mRNAs loses UPF1 binding in ZIKV-infected NPCs, consistent with UPF1's decreased expression using infrared crosslinking immunoprecipitation and RNA sequencing, as shown by UPF1's reduced expression. However, UPF1 target transcripts are not altered in abundance but in subcellular localization, with mRNAs accumulating in the nucleus of infected or UPF1 knockdown cells' nucleus.

Source link: https://doi.org/10.1371/journal.ppat.1011070


Development of a TaqMan minor groove binding probe-based quantitative reverse transcription polymerase chain reaction for the detection and quantification of Zika virus.

Following epidemic outbreaks of severe neurological disorders reported in Pacific and Americas since 2016, Zika virus infection has emerged as a global health issue. Therefore, a fast, sensitive, and specific diagnostic test for ZIKV infection is vital for patient diagnosis and disease prevention. The tested ZIKV qRT-PCR assay was tested for its detection limit, strain coverage, and cross-reactivity. Using a total of 18 simulated clinical specimens, we investigated the clinical use of qRT-PCR assay for ZIKV RNA detection. The detection limit of the qRT-PCR assay was 11. 276 ZIKV RNA copies at the 95% likelihood level, according to the 95% probability level. Both 100% and 100% respectively indicated that the qRT-PCR assay demonstrated a perfect match to the reference source; the sensitivity and specificity of the qRT-PCR assay were 100% and 100% respectively.

Source link: https://doi.org/10.47665/tb.39.4.005


Modeling the spread of the Zika virus by sexual and mosquito transmission.

Although approximately 80% of ZIKV infections are symptomatic and mild, multiple studies have shown a correlation between ZIKV and common illnesses such as Microcephaly and Guillain Barru00e9 Syndrome. Two objectives of this research are to develop ZIKV models by investigating the transmission dynamics of ZIKV, both a vector-borne and sexually transmitted disease epidemic and an indicator of under-reporting. Using the system, we find that targeting the sexual pathway alone has negligible effect on overall transmission, but if the number of risky sexual activity rises, it may become relevant. These findings may be helpful to public health agencies and governments to develop and implement appropriate health policies and minimize the Zika outbreaks' impact.

Source link: https://doi.org/10.1371/journal.pone.0270127


Mouse models of Zika virus transplacental transmission.

The ZIKV virus continues to circulate in low numbers long as the disease has existed for decades before the 2015 epidemic, but the exact date of the ZIKV outbreak is uncertain. Small animal models of ZIKV transplacental transmission have never been more essential to develop antiviral protocols for both mother and fetuses, as well as determining mechanisms of immunity at the maternal-fetal interface, in the void of ZIKV vaccines or antivirals.

Source link: https://doi.org/10.1016/j.antiviral.2022.105500


CD8+ T-cells trigger auricular dermatitis and blepharitis in mice post-Zika virus infection in the absence of CD4+ T-cells.

A significant decrease in viral load was shown by adoptive transfer of CD8+ T-cells in this research, which uses a T-cell deficient mouse model that maintains persistent ZIKV viral titres in the blood and organs. These causative CD8+ T-cells from the ears' single cell RNA sequencing of these causative CD8+ T-cells from the ears reveal an overactivated and elevated cytotoxic signature in mice with severe symptoms. Our findings support a role for CD8+ T-cell-associated pathologies post-ZIKV disease in CD4+ T-cell immunodeficient patients.

Source link: https://doi.org/10.1016/j.jid.2022.11.020


Comparative Analysis of In Vitro Models to Study Antibody-Dependent Enhancement of Zika Virus Infection.

Since the ZIKV outbreak endemic for the related dengue virus, it was believed that antibody-dependent improvement of a ZIKV virus, which were accompanied by the presence of cross-reactive DENV antibodies, may have contributed to ZIKV disease severity. We first determined which cell lines and primary cells could be used to study ZIKV ADE in vitro, and we compared the results of infection experiments between these cells by using a systematic approach. In comparison to non-pregnant women, there was no difference in permissiveness for ZIKV infection or ADE risk of ZIKV infection in pregnant women's primary cells. Using in vitro models of primary myeloid cells from pregnant women and age-matched non-pregnant women found no increased permissiveness for ZIKV disease and ADE of ZIKV infections in ZIKV disease and ADE of ZIKV infection.

Source link: https://doi.org/10.3390/v14122776

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions