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Although we previously stated that Zika virus could alter the function of bone forming osteoblasts, bone forming osteoblasts' growth, we are uncertain about OCs' susceptibility to ZIKV infection. We looked at the effects of ZIKV infection on osteoclastogenesis, and found that infected with ZIKV viruses in pre- and early OCs greatly reduced bone formation and bone resorption in osteoclast formation and bone resorption. Interestingly, pre-OCs with a low incidence ZIKV disease reacted with a high viral dose, suggesting a role for antibody-dependent enhancement in ZIKV-associated bone pathology.
Source link: https://europepmc.org/article/MED/35670284
Zika virus, a family member of the Flavivirus genus, has resurfaced as a global public health concern. Both virus generation and entry of flaviviruses are conducted by the envelope proteins of flaviviruses. To determine the key residues of E in virus entry, we created a ZIKV trans-complemented particle system, in which a subgenomic reporter replicon was packed with trans -complementation with an expression of CprME. Most mutated ZIKV TCPs had deficient egress, and the majority of mutated ZIKV TCPs had deficient egress. Mutations in DIII and in the hinge zone of DI and DIII affected prM expression. E trimerization was reduced in DIII by egress-deficient mutants in DIII, according to a bioorthogonal device and photocrosslinking experiments that showed crosslinked intracellular E trimers and demonstrated that egress-deficient mutants in DIII impaired E trimerization. Further virus binding experiments showed a deficiency of the mutated ZIKV TCPs in virus attachment.
Source link: https://europepmc.org/article/MED/35612559
Zika virus is mainly transmitted by mosquito bites and sexual contact, but vertical transmission of ZIKV has also been observed in humans. In addition, ZIKV infection transmitted by unknown routes has been frequently observed in clinical settings. However, it is also unknown if ZIKV can be spread via aerosol routes. Our results show that aerosolized ZIKV can cause systemic infection and trigger both innate and adaptive immune responses in guinea pigs, emphasizing the possibility of ZIKV transmission via aerosols.
Source link: https://europepmc.org/article/MED/36069671
Zika virus was isolated from Rhesus monkeys in Uganda forest, Africa, in 1947. Old World Primates are involved in a sylvatic cycle of maintenance of this arbovirus, but there is a lack of information about the role of New World primates in ZIKV transmission cycles. S100e3o Paulo, Paragua, Parau00edba, and Paranu00e1 Objective This study was designed to investigate the presence of enzootic circulation of ZIKV in New World Primates from three Brazilian states: Su00e3o Paulo, Paragua, and Paranu00e1. Results All samples were tested negative for the presence of ZIKV genes from tissue and blood samples, but not from blood and blood samples.
Source link: https://europepmc.org/article/MED/36074421
Optical stress in astrocytes, as well as dysregulations of neural cell proliferation and cell cycle, have been cited as important events determining congenital Zika Syndrome's cell death and neurological abnormalities. Copper deficiency has been shown to cause similar changes in other pathologies, and disruptions in copper homeostasis have already been described in viral infections. Here, we investigated copper homeostasis imbalance as a risk that might play a role in the cytotoxic effects of ZIKV infection in astrocytes. The effects of CuCl 2 or a copper chelator addition on oxidative stress, cell viability, and percentage of infection were also investigated. Eating micronutrient levels and the use of copper chelators in pregnant women exposed to ZIKV infection could be revealing ways to handle new cases of congenital ZIKV syndrome.
Source link: https://europepmc.org/article/MED/36063501
Despite the numerous research studies into Zika virus cure's drug discovery, no antiviral drugs or vaccines have been found, nor have any vaccines or vaccines have been produced. Due to increasing global travel, the mosquito vector and ZIKV infection transmission is expected to increase worldwide. It makes an NS3-Hel protein an attractive target for developing novel drugs for ZIKV therapy. The results from the molecular dynamics simulation results reveal dynamic stability between protein and ligand complexes, and the structures remained relatively unchanged at the simulation site during the simulation period.
Source link: https://europepmc.org/article/MED/36063275
The female reproductive tract's risk of sexually transmitted viral infections is not well understood by immunological surveillance methods. We'll discuss the importance of IFN-u03b5 for Zika virus prevention by: increased sensitivity of IFN-u03b5 mice,/- mice; u201c rescuee, u201c by intravaginal recombinant IFN-u01b5 therapy, and blockade of protective endogenous IFNu03b5 by neutralizing antibodies. IFNu03b5 had potent anti-ZIKV activity, was correlated with transcriptome responses similar to IFN, but lacking the proinflammatory gene signature of IFN u03b1 was shown by human FRT cell lines. These findings indicate that the unique spatiotemporal characteristics of IFNu03b5 provide an embedded immune surveillance network in the FRT, which is a significant barrier to viral infection in the FRT and has important implications for prevention and therapy. We'll explore the role of a hormonally controlled type I interferon, IFN epsilon, in the prevention of Zika virus infection of the FRT in this paper. Comparing to other canonical type-I IFNs, the IFN's IIIB5 is notable in that it is constitutively expressed by epithelial cells of the FRT with expression levels monitored by progesterone and not in response to viral infection. We demonstrate that IFNu03b5 mice, blockade of endogenous IFNâu03b5 by neutralizing Abs, and rescue of IFN KO mice by recombinant IFN u03b5 mice administered directly to the FRT by recombinant IFNu03b5. Despite their ability to antagonize the expression of IFNu03b2 or u03bb, ZIKV NS proteins did not inhibit IFNu03b5 expression, particularly because they were able to antagonize the expression of IFNu03b2 or u03bb.
Source link: https://europepmc.org/article/PPR/PPR539844
Objectivation: To determine whether or not having access to oral hygiene information influenced the behavior of caregivers of children with microcephaly caused by Zika virus. Most mothers reported receiving information about oral hygiene from their infants. According to Fisher's Exact and Pearson's Chi-square tests, access to oral hygiene data was related to mother's education, family income, regular visits to the dentist, frequency of tooth brushing, age when the infant was brushing, and brushing time. Conclusion With microcephaly caused by ZIKV, access to oral hygiene with oral hygiene positively influenced children's oral health care.
Source link: https://europepmc.org/article/MED/36048965
Importance Children with congenital Zika syndrome have been characterized by ocular lesions. Among children exposed to Zika virus during pregnancy, the incidence of ocular abnormalities is unknown. Objection: To determine its prevalence, we want to investigate ocular fundus manifestations in newborns with congenital ZIKV exposure in French Guiana, Martinique, and Guadeloupe, French West Indies, in order to determine its prevalence. The study's publication began in 2016 at the beginning of the ZIKV epidemic in the French West Indies' early. The presence of extraocular fetopathy in Lesions capable of congenital ZIKV infection were not consistent with extraocular fetopathy. Microcephaly was not connected to lesions that would not be appropriate for congenital ZIKV infection, but severe microcephaly was associated with an OR of 81.
Source link: https://europepmc.org/article/MED/36048466
Background The recognition of viral infection by an inherent antiviral immune system infects the type I interferon and proinflammatory signaling pathways. Results Here are the results We have discovered that the TRIM22 has been significantly reduced both transcriptionally and translationally depending on Zika virus infection. ZIKV infection in humans is characterized by mild to moderate signs of infantile brain development, including abnormal fetal brain development. The antiviral function of TRIM22, we discovered, is essential in fighting ZIKV infection. Overexpression of TRIM22 protein stifled ZIKV growth, while deletion of TRIM22 in host cells raised ZIKV infectivity. TRIM22, a fully integrated E3 ubiquitin ligase, promoted the ubiquitination and degradation of ZIKV nonstructural protein 1 and nonstructural protein 3. In addition, we discovered that TRIM22 also inhibited other flavivirus infections, including dengue virus and yellow fever virus.
Source link: https://europepmc.org/article/MED/36042495
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