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Zika Virus - Astrophysics Data System

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Last Updated: 09 January 2023

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In silico construction of a multiepitope Zika virus vaccine using immunoinformatics tools

Zika virus is an arbovirus from the Flaviviridae family and the Flavivivirus genus. With the rise of ZIKV infection prevalence in newborns in 2015, infant mortality and microcephaly infections have increased sharply, indicating congenital transmission. Hence, the development of an effective vaccine against ZIKV has been a critical need. Based on the number of different HLA alleles that bind to the epitopes, promiscuity based on the number of different HLA alleles that bind to the epitopes, and immunogenicity, we selected 13 CD8 + and 12 CD4 + T cell epitopes. The ZIKV Envelope protein domain III was added to the vaccine design, creating a hybrid protein domain-multiepitope vaccine. We investigated secondary and tertiary structures and physicochemical characteristics of the vaccine conjugated to four different protein adjuvants, including flagellin, a hemparin-binding hemagglutininin, or RS09 synthetic peptide. Individuals with previous ZIKV infection were screened for cytokine production in response to a pool of CD4 and CD8 ZIKV peptides. After stimulation, IFN-u03b3 production was seen by T cells and cells, and CD8 + T cells detected IL-2 production, which indicated that our peptides could be recognized by specific T cells and stimulate immune responses. In conclusion, we developed a silico universal vaccine that can produce broad and high-coverage cellular and humoral immune responses against ZIKV, making it a good candidate for posterior in vivo validation.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..12...53A/abstract


Zika virus baculovirus-expressed envelope protein elicited humoral and cellular immunity in immunocompetent mice

We created a recombinant vaccine utilizing the baculovirus expression system with two strains of ZIKV envelope protein, because vaccination is the most cost-effective defense against ZIKV infection. We recommend that the baculovirus expression device ZIKV envelope protein recombinant provides a safe and effective vaccination program.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..12..660S/abstract


Vulnerability of primitive human placental trophoblast to Zika virus

We've tested the possibility that early pregnancy's placenta may be more easily affected by the Zika virus than the mature placenta's more resilient outer cells. We conclude that the human fetus may be the most vulnerable to ZIKV early in pregnancy, and that the African strain may cause a more threatening pregnancy than previously expected.

Source link: https://ui.adsabs.harvard.edu/abs/2017PNAS..114E1587S/abstract


Intracellular translocation of HMGB1 is important for Zika virus replication in Huh7 cells

Zika virus infection causes severe problems in infant and adult Guillain-Barru00e9 syndrome. The potential role of high mobility group box 1 protein in ZIKV infection was investigated in the current study. The enzyme-linked immunosorbent assay and immunoblot assay established HMGB1 protein expression by enzyme-linked immunosorbent assay and immunoblot assay. The Huh7 cells were extremely susceptible to ZIKV infection, according to the researchers. The infection was found to lead to a 99% rise in the cytosolic HMGB1 expression at 72 hours post-infection. With a maximum decrease of 71 u00b1 5. 84%, treatment of the ZIKV-infected cells with dexamethasone reduced HMGB1 extracellular release in a dose-dependent manner. By over 83 percent, titers were also reduced by more than 80%. u00b1 0. 55 percent — These findings indicate that translocation of HMGB1 occurred during ZIKV infection and impeded translocation by dexamethasone coincided with a decrease in ZIKV replication, which led to a decrease in ZIKV replication.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..12.1054C/abstract


Zika virus employs the host antiviral RNase L protein to support replication factory assembly

An increased understanding of ZIKV's use of host factors to promote infection would increase our understanding of the subcellular events that contribute to ZIKV disease's clinical manifestations, as well as potential avenues for therapeutic and vaccine development to combat the virus.

Source link: https://ui.adsabs.harvard.edu/abs/2021PNAS..11801713W/abstract


Zika virus noncoding RNA cooperates with the viral protein NS5 to inhibit STAT1 phosphorylation and facilitate viral pathogenesis

We also show that sfRNA promotes apoptosis of neural progenitor cells in human brain organoids, contributing to their disintegration. Further research shows that sfRNA gene production leads to reduced phosphorylation and nuclear translocation of STAT1 via a mechanism that involves sfRNA binding to and stabilizing viral protein NS5. Our findings indicate that viral noncoding RNA and a viral protein may work together as a novel tactic for combating antiviral responses. Noncoding RNA of Zika virus allows for viral escape of antiviral responses in vertebrates.

Source link: https://ui.adsabs.harvard.edu/abs/2022SciA....8D8095S/abstract


The RNA polymerase of cytoplasmically replicating Zika virus binds with chromatin DNA in nuclei and regulates host gene transcription

The ZIKV NS5, the viral RNA polymerase that is essential for viral replication in the cytoplasm, can bind with chromatin DNA in the nuclei, according to this article. Our results reveal a role for ZIKV RdRp as a DNA binding protein that regulates host gene transcription and provide insight into abnormal neural development and ZIKV infection.

Source link: https://ui.adsabs.harvard.edu/abs/2022PNAS..11905013L/abstract


Estimating Zika virus attack rates and risk of Zika virus-associated neurological complications in Colombian capital cities with a Bayesian model

For 28 capital cities in Colombia, we used multiple data sources to help estimate ZIKV infection attack rates, including outbreaks of Zika virus disease and the possibility of ZIKV-associated NC. Per 10 000 ZIKV infections, the estimated ZVD reporting rate was 0. 13 percent, with 0. 51 cases of ZIKV-related NC reported per 10 000 ZIKV infections. When we assumed the same ZIKV IAR across sex or age group, we found significant spatial differences in ZVD's reporting rates and the danger of being identified as a ZVD case with NC in ZVD.

Source link: https://ui.adsabs.harvard.edu/abs/2022RSOS....920491C/abstract


Modeling the spread of the Zika virus by sexual and mosquito transmission

Although nearly 80% of ZIKV infections are symptomatic and mild, several studies have shown a correlation between ZIKV and common diseases such as Microcephaly and Guillain Barru00e9 Syndrome, which are related to common illnesses. We find that focusing the sexual pathway alone has negligible effects on overall dissemination of disease transmission, which is negligible, but if the percentage of risky sexual activity rises, it could become significant. These findings can be helpful to public health departments and governments to design and implement appropriate health care strategies in order to minimize the Zika outbreaks' impact.

Source link: https://ui.adsabs.harvard.edu/abs/2022PLoSO..1770127A/abstract

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions