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So we investigated the effects of AZT on embryo formation, TL, and copy number of an active TE, Long Interspersed Nuclear Element 1 (LIE) during early development in a murine model. Methods In vivo fertilized mouse zygotes from B6C3F1/B6D2F1 mice were cultured for 48 h in KSOM with no AZT, AZT 1 M, or AZT 10 M. Among organizations, the percentage of morulas at 48 h, TL, and LINE-1 copy number was compared to others. The TL in AZT 1 M embryos is shorter than in control embryos, and it's shorter than in control embryos. Compared to oocytes controls, AZT 1 M increases LINE-1 copy number, and AZT 10 M embryos. After 48 hours of treatment, AZT at concentrations approaching those used to reduce perinatal HIV transmission compromises mouse embryo development, prevents telomere elongation, and increases LINE-1 copy number.
Source link: https://doi.org/10.1007/s11033-021-06788-x
Background Zidovudine is the most commonly used antiretroviral drug. It has been used in antiretroviral therapy. Objectives: To determine the permeability of AZT through the skin, two permeation enhancers, sonophoresis, and microneedles are used. Methods Permeation experiments using an AZT solution were carried out using pigskin clamped in Franz-type cells. Sonophoresis was administered under various conditions, including the rise in the skin surface temperature and the rise in transepidermal water loss, which were determinable in an experimental setting. ATR-FTIR was also used to demonstrate the effect of enhancers on membrane structures. Conclusions The permeability of AZT through intact skin was extremely poor, with a lengthy lag time. Pretreatment of the skin with sonophoresis raised AZT transport significantly, reducing the lag time.
Source link: https://doi.org/10.1007/s40199-021-00402-y
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