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Western Blot - PubMed

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Last Updated: 09 January 2023

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An optimized western blot assay provides a comprehensive assessment of the physiological endoproteolytic processing of the prion protein.

The prion protein is exposed to several conserved endoproteolytic reactions that have increased demand for their bodily function and their involvement in the pathogenesis of prion diseases and other neurodegenerative disorders. In a mixed biological sample, we were able to obtain the most detailed PrPC constitutive processing and the relative abundance of PrPC proteoforms. Interestingly, the relative amount of shed PrPC in wt mice was higher in wt mice than in most other models. PrPC's overexpression or host factors other than PrPC are not affected by PrPC overexpression or host factors other than PrPC, according to our results, but PrPC primary structure can be affected by PrPC primary structure.

Source link: https://doi.org/10.1016/j.jbc.2022.102823


Development and Validation of a Western Blot Method to Quantify Mini-Dystrophin in Human Skeletal Muscle Biopsies.

Duchenne muscular dystrophy is a degenerative muscular disorder that affects approximately one in 5000 males at birth. New discoveries in gene therapy and knowledge of dystrophin expression in other muscular dystrophies have opened new avenues for treatment. DMD and Becker muscular dystrophy are both common muscular dystrophies and evaluate the effectiveness of new therapies for muscular dystrophies. Here, we discuss the testing of a new Western blot technique for the quantitation of mini-dystrophin protein in human skeletal muscle tissue samples that is easy to use in most laboratory settings. Protein extraction from skeletal muscle tissue lysates from control, DMD, or BMD biospecimens is the first step in Step 1. With housekeeping GAPDH, Step 3 involves running gel electrophoresis with wild-type dystrophin from muscle tissue extracts along the mini-dystrophin STD curve and mini-DYS curve and protein normalization.

Source link: https://doi.org/10.1208/s12248-022-00776-0


Western blot using Trypanosoma cruzi chimeric recombinant proteins for the serodiagnosis of chronic Chagas disease: A proof-of-concept study.

Trypanosoma cruzi causes Chagas disease in Chagas patients. The presence of IgG anti-T. cruzi antibodies in the chronic phase of CD is characterized by the presence of IgG anti-T. cruzi antibodies; and diagnosis is carried out by serological techniques. Because there is no such thing as a reference measure, WHO recommends the simultaneous use of two separate tests for CD serodiagnosis. Using a WB testing platform, the present study looked at the potential of these molecules to diagnose chronic CD despite its high diagnostic success.

Source link: https://doi.org/10.1371/journal.pntd.0010944


Simultaneous detection of antibody responses to multiple SARS-CoV-2 antigens by a Western blot serological assay.

The nucleic acid test is still the most widely used diagnostic tool for the diagnosis of coronavirus disease 2019, which is caused by human infection of severe acute respiratory syndrome coronavirus 2. Multiple Escherichia coli-expressed SARS-CoV-2 antigens were used in this research for comprehensive analyses of antibody profiles in COVID-19 patient sera. The results of S, S2, and N were detected by the multi-antigen-coated WBS-based serological assay, as well as a positive correlation with the plaque reduction neutralization test assays' positivity. The detection signals against the unstructured receptor-binding domain purified from E. coli inclusion bodies were not observed in the PRNT assays, although the COVID-19 patient sera demonstrated high neutralizing potency in the PRNT assays, showing that patient serum RBD-specific antibodies generally recognize the conformational epitopes.

Source link: https://doi.org/10.1007/s00253-022-12288-0


Core-shell SERS nanotags-based western blot.

The most commonly used protein identification scheme in life science, Western blot, is the most commonly used method for protein identification, but it still faces significant challenges in the accurate quantitative measurement of low-abundance proteins. The SERS-WB's results show that the SERS-WB demonstrated a high detection rate of glyceraldehyde-3-phosphate dehydrogenase protein at 0. 15 pg, as well as a wide dynamic range from 382 fg to 382 ng.

Source link: https://doi.org/10.1016/j.talanta.2022.123888

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions