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Synthetic mesenchymal stem cells have a high proliferative capability and are regarded as a valuable source for stem cell therapy. Aims: To isolate MSCs from ankle joints in patients with persistent lateral ankle pain, and to compare MSCs from the synovium anterior to the talus with those from the immediate anterior talofibular ligament synovium. Methods The synovium was obtained from two places in the ankle, the synovium anterior to the talus, and the immediate ATFL synovium of 14 patients undergoing arthroscopic ATFL repair for CLAI without osteochondral lesions of the talus. The Wilcoxon signed rank test was used to compare the sample weight, number of colonies, number of nucleated cells per colony, yield, and phenotypic characteristics of MSCs obtained from various locations of the synovium. Compared to cells from the surrounding ATFL synovium cells, the frequency of oil red O-positive adipogenic colonies and the expression of the adipsin gene were significantly higher in the cells from the synovium anterior to the talus. Synovial MSCs obtained from the ankle joint displayed self-renewal and multilineage differentiation potential, but the adipogenesis ability of MSCs from the synovium anterior to the talus was superior to that from the surrounding ATFL synovium. Both the adipose synovium and fibrous synovium in patients with CLAI's ankle joints may be a good source of MSCs for stem cell therapy applications, although synovial fluid was unsuitable.
Source link: https://europepmc.org/article/MED/35601732
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