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Stem cell function - Wiley Online Library

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Last Updated: 27 January 2022

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Stage‐Specific Generation of Human Pluripotent Stem Cell Derived Lung Models to Measure CFTR Function

Human embryonic stem cells and induced pluripotent stem cells, as well as in vitro human lung epithelial cells, are useful tools that can be produced in vitro human lung epithelial cells. Here, we optimize our previously published protocols to produce three developmental stages of the lung model's robustness and demonstrate its capability for cystic fibrosis drug discovery platforms. The article also discusses the production of adultlike airway epithelial cells in Matrigel in conjunction with a magnetic-activated cell sorting system, as well as the generation of adultlike airway epithelial cells in the form of epithelial cells. Basic Protocol 1: hESC/hiPSC differentiation to fetal lung progenitors Basic Protocol 2: Cryopreservation of airway epithelial spheroids in ALI culture Alternate Protocol 1: hESC/hiPSC differentiation to fetal lung progenitors Basic Protocol 2: Origination and maturation of epithelial spheroids Basic Protocol 2: Cryopreservation of epithelial sphethelial s Basic Protocol 1: Cryoproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproproprothelial sphethelial spheroids sphethelial spheco.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/cpz1.341


Cytology, function and dynamics of stem and progenitor cells in decapod crustaceans

The Decapoda embryos and larvae, as well as in the epidermis, musculature, heart, brain, liver, kidney, hepatopancreas, olfactory sense organs, and adult gonads are among the adult's epidermis, mosquito and functionally different stem cells in embryos and larvae. The available data shows that the Decapoda have a remarkable number of functionally and larvae Our decapods' understanding of tissue formation, growth, and preservation of organs is largely improved due to the discovery, expansion, and maintenance of tissues and organs in this species. Cultured decapod stem cells have a high potential for toxicity testing and virus study in aquaculture with practical significance for aquaculture. The devolution of stem cells and regeneration in the animal kingdom also increases our knowledge of stem cell evolution and regeneration. The stem cells of long-lived, indeterminately growing decapods may be the key to understanding how stem and progenitor cells respond in old age without adverse side effects, possibly spurring new approaches for the manufacture of anti-ageing and anti-cancer therapies in humans.

Source link: https://onlinelibrary.wiley.com/doi/10.1111/brv.12824


From quiescence to repair: C/EBPβ as a regulator of muscle stem cell function in health and disease

Herein, we discuss a role for C/EBP as a regulator of skeletal muscle stem cell function. C/EBP/Personal Expression of stem cell self-renewal and C/EBP are both required for mitotic quiescence in this cell population. C/EBP/expression is elevated in pathological disorders such as cancer cachexia, which disturbs muscle repair and promotes myofiber atrophy in the context of systemic inflammation. We introduce a role for C/EBP in muscle stem cells in this way, and we suggest a functional intersection between C/EBP/NFKB intervention in cancer cachexia regulation.

Source link: https://onlinelibrary.wiley.com/doi/10.1111/febs.16307

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