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Stem cell Function - Europe PMC

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Last Updated: 27 April 2022

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Human pluripotent stem cell-derived cells endogenously expressing follicle-stimulating hormone receptors: modeling the function of an inactivating receptor mutation.

The FSHR's actions have mainly been investigated in vivo models, primary cells, cancer cells, and cell lines ectopically expressing the FSHR. We separated FSHR-expressing cells from human pluripotent stem cells to facilitate endogenous FSHR function in vitro. We investigated protein-protein interaction partners of FSHR in resting state and post FSH stimulation, producing FSHR in HEK293 cells followed by affinity purification mass spectrometry analysis. In conclusion, our protocol provides detailed analysis of FSH action and disease modeling in human cells endogenously expressing FSHR.

Source link: https://europepmc.org/article/MED/35471239


Reactive Oxygen Species in Modulating Intestinal Stem Cell Dynamics and Function.

Intestinal stem cells that reside in crypt-specific niches play an important role in gut epithelium regeneration. ISC also supports the development of mature intestinal epithelial cells by regular differentiation and renewal in short turnover cycles. Interestingly, the gut's oxidative stress has a major role in the regulation of ISC proliferation and development. All repair techniques that aid in the growth of the gut through constant repair and replacement are based on Intuitive stem cells. One of the primary factors that influence ISC formation is reactive Oxygen Species. ROS content in the gut can directly influence stem cell regeneration ROS itself, but many other factors, such as the microbiota affecting the gut and immune cells, can also influence one another by various cross-talk mechanisms, influence one another.

Source link: https://europepmc.org/article/MED/35461466


Enhancing and stabilization of cord blood regulatory T cell suppressive function by human mesenchymal stem cell (MSC)-derived exosomes.

T cells that regulate peripheral tolerance and immune homeostasis are essential to maintaining peripheral tolerance and immune homeostasis. We investigated the possibility of mesenchymal stem cell-derived exosomes in this study to promote and maintain Tregs function. Tregs were more inhibitive in both polyclonal and allogeneic responses and were resistant to inflammatory stimulation in vitro relative to the controls, and were resistant to inflammatory stimulation in vitro. A similar improvement in Treg function was also observed by culturing Tregs with MSC-derived exosomes alone. Exosomes were depleted from MSC-CM, dramatically decreasing Treg culture and stability. We discovered that MSC-derived exosomes could upregulate the expression of LC3, phosphorylate Jak3 and Stat5 to promote Treg survival, and limit FOXP3 expression in Tregs. Our results support the use of MSC-derived exosomes as a cost-effective way to increase Treg function and raise the overall Treg-based cell therapy landscape.

Source link: https://europepmc.org/article/MED/35439818


RAC1B function is essential for breast cancer stem cell maintenance and chemoresistance of breast tumor cells

Breast cancer stem cells have been attributed to breast cancer resistance, tumor recurrence, and metastasis of breast tumors. Unlike Rac1, Rac1b mode is dispensable for normal mammary gland formation and mammary epithelial stem cell activation. In contrast, lack of Rac1b function in a mouse model of breast cancer slows BCSC development in vivo and boosts primary tumor cell sensitivity to doxorubicin.

Source link: https://europepmc.org/article/PPR/PPR482877


Enhancement of pacing function by HCN4 overexpression in human pluripotent stem cell-derived cardiomyocytes.

Background The number of patients with bradyarrhythmia and the number of patients with cardiac pacemakers are on the rise with the aging population and the rise in the number of heart disease patients. Some patients in whom a cardiac pacemaker has been implanted have problems such as pacemaker infection and discomfort as a result of electromagnetic interference. We have found that overexpression of HCN channels resulting in a pacemaker current in mouse embryonic stem cell-derived cardiomyocytes enhanced pacing function in vitro and in vivo. The aim of this research was to determine whether HCN4 overexpression in human-induced pluripotent stem cell-derived cardiomyocytes could improve the cells's pacing function. Methods Human HCN4 was transduced in the AAVS1 locus of human induced pluripotent stem cells by nucleofection by nucleofection, and HCN4-overexpressing iPSC-CMs were produced. HCN4-overexpressing cardiomyocytes were also higher in HCN4-overexpressing iPSC-CMs than in non-overexpressing iPSC-CMs.

Source link: https://europepmc.org/article/MED/35365232


Adipose-derived stem cell and their extracellular vesicles ameliorates immune function, and cardiac markers in experimental model of cardiorenal syndrome type III: TNF-α, IFN-γ and IL-10 cytokine production and their correlation with genotype.

In an experimental model of cardiorenal syndrome type III, the current research is done to determine the anti-inflammatory role of adipose tissue-derived stem cells versus adipose stem cell-derived extracellular vesicles. Group I was equally divided into: group I; Group II, which was triggered by right renal artery ligation; Group III, which underwent surgical incision without renal artery ligation; Group IV ICRSIII was given adipose tissue stem cells; Group IV ICRSIII was divided into group I; group II; Group II; Group III was identified as adipose tissue stem cells; Group V ICRSIII; and Group III was determined without renal ligation In the ICRSIII group, proinflammatory cytokines, BNP, and NGAL were all significantly elevated, relative to the other study groups. With no significant difference between them, administration of ADSCs-EVs or ASCs resulted in significant decline in all proinflammatory cytokines, as well as BNP and NGAL levels, which were not noticeable differences between them.

Source link: https://europepmc.org/article/MED/35364243

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions