Advanced searches left 3/3

Stem cell Function - Wiley Online Library

Summarized by Plex Scholar
Last Updated: 27 July 2022

* If you want to update the article please login/register

Compatibility and function of human induced pluripotent stem cell derived cardiomyocytes on an electrospun nanofibrous scaffold, generated from an ionomeric polyurethane composite

The functional properties of the hiPSC-u2010CM cultured on gelatin and Matrigel scaffolds were investigated, as well as the effect of protein interactions with the synthetic substrate on subsequent cell phenotypes. The cells on the aligned scaffold were elongated and developed synchronized sarcomeres that oriented parallel to the fiber direction, while the cells on random scaffolds and a tissue culture polystyrene control did not have such morphological characteristics. The hiPSC-u2010T composite scaffolds and TCPS generated similar amounts of cardiac troponin, u2010T, but there was a higher incidence of ventricular myosin light chain u20102, suggesting a higher proportion of hiPSCu2010CMs with a ventricular cardiomyocyte like phenotype. These preliminary findings show that coordinated Du2010PHI elastomeric scaffolds allow hiPSCu2010CMs to exhibit important cardiomyocyte characteristics, which is crucial to enabling cardiac tissue regeneration.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37428


Direct administration of mesenchymal stem cell‐derived mitochondria improves cardiac function after infarction via ameliorating endothelial senescence

Following infarction, the replacing of injured mitochondrial bioenergy in myocardium with a healthy mitochondrial system provides a significant threat to cardioprotection. In a mouse model of myocardial infarction compared to mitochondria derived from skin fibroblast cells and to determine the underlying mechanisms of their effects on endothelial cells, this research sought to determine whether direct transplantation of exogenous mitochondria obtained from mesenchymal stem cells is both efficient and superior in protecting cardiac function. In a mouse MI model, direct injection of MSCu2010mt into the peri-u2010infarct region increased blood vessel density, inhibited cardiac remodeling, and apoptosis, thus improving heart function in comparison to the FBu2010 milliont group. After coculture, the fluorescence signal of MSC 2010mt can be seen in human umbilical vein endothelial cells by confocal microscopy and flow cytometry. These results show that using MSCs as mitochondrial sources is safe, and that proangiogenesis could be the mechanism by which MSC-u2010mt transplantation attenuates MI.

Source link: https://onlinelibrary.wiley.com/doi/10.1002/btm2.10365


Normal mesenchymal stem cells can improve the abnormal function of T cells in psoriasis via upregulating transforming growth factor‐β receptor

Previous research has found that the T cells of T cells in psoriatic patients' immune imbalance are T cells, while mesenchymal stem cells' (TR) genes were linked to a decrease in the number of Th17/Treg. The use of mesenchymal stem cells is a new treatment for immune disorders. Normal DMSC were more potent than psoriatic DMSC in inhibiting CD3+ Tu2010cell proliferation inhibition, T3+ Tu2010cell formation inhibition, and T3+ Tsoriasis stimulation were more potent than psoriatic DMSC in inhibition of CD3+ Tissue proliferation inhibition, as well as boosting CD3+ Tu2010cell apoptosis stimulation was greater than psoriatic DMSC inhibi In addition, the normal DMSC were more potent than the psoriatic DMSC in increasing TGF levels and stimulating the TGF/SMAD pathway in psoriatic CD3+ T cells, while the SMAD pathway was more active in phosphatiatic CD3+ T cells. Normal DMSC might partially enhance the biologic function and immunosuppressive capacity of psoriatic CD3+ T cells, perhaps by upregulating the TGFu2010. u03b2 receptors.

Source link: https://onlinelibrary.wiley.com/doi/10.1111/1346-8138.16490

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions