Advanced searches left 3/3

Stem cell Function - Europe PMC

Summarized by Plex Scholar
Last Updated: 27 July 2022

* If you want to update the article please login/register

Preclinical investigation of expanded human adipose–derived stem cell dosage and timing for improved defecation function in immunodeficient mice

Adipose-derived stem cells have emerged as promising therapeutic agents for anal function, but most preclinical trials of their use for anal function have used autologous or allogeneic ADSCs. In three groups: ADSC, phosphate-buffered saline, and control, were all used to induce anal sphincter injury in immunodeficient mice. Among groups using defecation status and pathological examination, the effects of differing timing and numbers of hADSCs administered were compared. Results In terms of defecation status, groups receiving u2265 1 hADSCs immediately after injection showed improvement. The sphincter's thinnest part of the sphincter was thicker in those treated with 1 tup3 hADSCs or PBS, according to pathological reports. Conclusions: The hADSC therapy in a mouse model of an anal sphincter injury was safe.

Source link: https://europepmc.org/article/PPR/PPR521430


Compatibility and function of human induced pluripotent stem cell derived cardiomyocytes on an electrospun nanofibrous scaffold, generated from an ionomeric polyurethane composite.

The hiPSC-CM cultured on gelatin and Matrigel-coated scaffolds was investigated, determining the effect of protein interactions with the synthetic substrate on subsequent cell phenotypes. The cells on the aligned scaffold were elongated and displayed aligned sarcomeres that oriented parallel to the fibers' direction, although those on random scaffolds and a tissue culture polystyrene control did not have such morphology. The hiPSC-CMs cultured on the scaffolds and TCPS had similar values of cardiac troponin-T, but there was a higher incidence of ventricular myosin light chain-2 on the D-PHI composite scaffolds relative to TCPS, indicating a larger proportion of hiPSC-CMs with a ventricular cardiomyocyte-like phenotype. These preliminary findings show that aligned D-PHI elastomeric scaffolds help hiPSC-CMs maintain important cardiomyocyte characteristics, which could lead to their future use for cardiac tissue regeneration.

Source link: https://europepmc.org/article/MED/35851742


Acetyl-CoA-Carboxylase 1-mediated de novo fatty acid synthesis sustains Lgr5 + intestinal stem cell function.

However, although the role of cellular fatty acid oxidation is well known, it is not clear how de novo fatty acid synthesis influences IECs' biology. We note here that interfering with de novo FAS by deletion of the enzyme Acetyl-CoA-Carboxylase 1 in IECs resulted in the loss of epithelial crypt structures and a specific decrease in Lgr5 + intestinal epithelial stem cells. Lastly, inhibition of ACC1 promotes tumor formation in colitis-associated colon cancer, thus highlighting the importance of cellular lipogenesis for maintaining ISC function and suggesting a potential path to colon cancer therapy.

Source link: https://europepmc.org/article/MED/35810180


Myoscaffolds reveal laminin scarring is detrimental for stem cell function while sarcospan induces compensatory fibrosis

ABSTRACT We developed an on-slide decellularization strategy to produce acellular extracellular matrix scaffolds that can be repopulated with various cell types in order to determine cell-ECM interactions. We investigated whether fibrotic ECM scarring affected human skeletal muscle progenitor cell functions that are essential for myoregeneration by using this platform. Our in vivo results showed that ECM remodeling is extremely important for SMPC graftonation, and that fibrotic scars may be one barrier to effective cell therapy.

Source link: https://europepmc.org/article/PPR/PPR515270


Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying MicroRNA-29a Improves Ovarian Function of Mice with Primary Ovarian Insufficiency by Targeting HMG-Box Transcription Factor/Wnt/ β -Catenin Signaling.

affluent insufficiency is a female disease characterized by ovarian function deficiency in children under the age of 40. Transplantation of exosomes is an effective regenerative medicine technique that has the potential for restoring ovarian functions post-POI with high success. We therefore investigate the clinical effectiveness and potential mechanisms of human umbilical cord mesenchymal stem cell-derived exosomes on ovarian dysfunction post-POI. GC proliferation and profitability were measured using 5-ethynyl-2'-deoxyuridine assays and Cell Counting Kit-8, and GC apoptosis was determined by flow cytometry. The luciferase reporter assays that the miR-29a and the HMG-box transcription factor had been established. Upregulation solved the miR-29a upregulation-induced GC apoptosis in GC apoptosis by increasing the Wnt/u03b2 -catenin pathway, as well as inactivating the Wnt/ u03b2 -catenin pathway. The exosomal miR-29a derived from human UCMSCs improves the ovarian function by targeting HBP1 and inducing the Wnt/u03b2 -catenin pathway.

Source link: https://europepmc.org/article/MED/35845134


Editorial Commentary: Stem Cell Exosomes Can Promote Healing and Muscle Function After Rotator Cuff Repair.

Exosomes are an innovative emerging tissue therapy approach for the delivery of stem cell-derived growth factors and small molecules to a regenerative tissue environment. Stem cells themselves may not engraft into host tissue, but they may more likely contribute to a pro-regenerative state by the introduction of autocrine and paracrine factors. Exosomes, a small membrane-bound extracellular vesicle secreted by cells, are of concern as an emerging therapeutic strategy because they contain several key elements that may be able to achieve the elusive goal of stem cell therapy in a more effective manner. Exosomes from stem cells seem to have a large amount of the machinery that could promote regenerative capabilities, including growth factors, micro-RNAs, and other signaling molecules that can cause the necessary growth signaling and transcriptional changes to cause a local delivery environment. Exosome-based therapeutic solutions for rotator cuff repair may be a promising treatment option for these reasons.

Source link: https://europepmc.org/article/MED/35809976

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions