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This research was focused on the use of human Placental mesenchymal stem cells-exosome in an animal model of severe SCI under a new myelogram technique to determine lumbar puncture injection accuracy and measure intrathecal space. In the tissue samples collected from the injury site's tissue samples, immunohistochemistry of GFAP and NF200 factors, as well as the apoptosis tunnel test, was investigated. Conclusions The use of the myelogram proved that it was a quick, convenient, safe, and cost-effective way to determine the accuracy of therapeutic agents LP injection and examination of the subarachnoid space in the model of laboratory animals. Other benefits of the exosome's include improved functional recovery by not producing bedsores in the treatment group and avoiding hematuria.
Source link: https://doi.org/10.21203/rs.3.rs-1863418/v1
This research will examine two specific blocking techniques, namely adsorption of albumin from bovine serum and grafting of small polyethylene glycol chains to titanium, which is the gold standard metal for orthopedics and dentistry, but not the gold standard metal for orthopedics and dentistry. The biological response of human mesenchymal stem cells was investigated by determining the number of attached cells and measuring cell proliferation on the substrate. Cell fate is not affected by the blocking technique; cell adhe and spread more on the functionalized samples, regardless of the blocking technique used. Both aspects of cell behavior are not significantly affected by the blocking technique: cells adhere and spread more on the functionalized samples; regardless of the blocking technique used. BSA coating has been shown to be as effective as BSA coating in reducing non-specific interactions and not minimizing the biomolecule's influence. However, considering the numerous benefits of a chemical and customizable polymer chain over a complex natural protein, PEG blocking stands out as a particularly effective alternative to albumin adsorption.
Source link: https://doi.org/10.5821/sibb.22.1.4802
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