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Sickle Cell Disease - PubAg

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Last Updated: 16 October 2021

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Dielectric spectroscopy of red blood cells in sickle cell disease

Hypoxia‐induced polymerization of sickle hemoglobin and the related ion diffusion across cell membrane layer can cause modifications in cell dielectric properties, which can potentially function as label‐free, analysis biomarkers for sickle cell disease. The outcomes of sickle cells reveal that on hypoxia treatment, cell inside permittivity and conductivity boost, while cell membrane layer capacitance lowers. On the other hand, the changes in typical red blood cells in between the hypoxia and normoxia states are unnoticeable. The outcomes of sickle cells might act as a recommendation to design dielectrophoresis‐based cell sorting and electrodeformation testing gadgets that need cell dielectric attributes as input specifications. The demonstrated method for dielectric characterization of single cells from the resistance spectroscopy of cell suspensions can be potentially put on various other cell types and under diverse gas problems.

Source link: https://pubag.nal.usda.gov/catalog/7298871


Allele-Specific Recombinase Polymerase Amplification to Detect Sickle Cell Disease in Low-Resource Settings

Sickle cell disease is a group of typical, dangerous disorders triggered by a point mutation in the β globin gene. Here, we describe the development of a quick, low-cost nucleic acid test that makes use of real-time fluorescence to spot the factor mutation inscribing hemoglobin S in one round of isothermal recombinase polymerase boosting. When evaluated with a set of scientific examples from SCD patients and healthy and balanced volunteers, our assay demonstrated 100% level of sensitivity for both the βᴬ globin and βS globin alleles and 94. 7 and 97. 1% specificities for the βᴬ globin allele and βS globin allele, respectively. This study demonstrates the possible utility of a quick, sample-to-answer nucleic acid examination for SCD that might be implemented near the factor of care and can be adjusted to other disease-causing point mutations in genomic DNA.

Source link: https://pubag.nal.usda.gov/catalog/7317613


Nutritional perspectives on sickle cell disease in Africa: a systematic review

BACKGROUND: Sickle cell disease is an acquired blood problem that predominantly impacts people in sub-Saharan Africa. Nevertheless, research that illuminates links in between SCD pathophysiology and dietary condition in African patients is lacking. Inclusion standards consisted of original, peer-reviewed research released in between January 1995 and November 2020 including people in Africa with any kind of phenotypic variant of SCD and a minimum of one nutritional condition result. Just 4 randomized tests were recognized, which determined the impact of lime juice, long-chain fats supplements, ready-to-use supplemental food, and oral arginine on wellness outcomes. FINAL THOUGHTS: The findings expose a modest number of descriptive studies, many with small example dimensions, that focused on various aspects of nutrition and SCD in African patients. There is an unmet demand for medical research to much better comprehend the potential advantages of nutrition-related interventions for patients with SCD in sub-Saharan Africa to promote optimum development and enhance wellness end results.

Source link: https://pubag.nal.usda.gov/catalog/7312123


Nanoscale adhesion profiling and membrane characterisation in sickle cell disease using hybrid atomic force microscopy-IR spectroscopy

Sickle cell disease provides a substantial international illness. The present work makes use of the hybrid technique of atomic pressure microscopy nano-infrared spectroscopy to probe modifications to the coefficient of friction and C-O IR strength in SCD on a nanoscale for dried out red cell taken care of under conditions of hypoxia and associates these monitorings with sticky communications at the membrane. Using functionalised AFM pointers, it has been feasible to probe glue communications between hydrophilic and hydrophobic moieties exposed at the surface of the dried RBCs taken care of under different oxygenation states and for different cell genotypes. The outcomes follow higher PS-exposure and oxidative damages in hypoxic sickle cells, as formerly recommended, and additionally show solid correlation in between localized oxidative damage and raised attachment.

Source link: https://pubag.nal.usda.gov/catalog/7139930


93 A sheep model of sickle cell disease using CRISPR/Cas9 and somatic cell nuclear transfer

Sickle cell disease is one of the most usual acquired hemoglobinopathy, with greater than 2 million people in the United States alone bring the sickle genetics. SCD is brought on by a single A to T nucleotide substitute at the 6th codon of the β-globin gene, which leads to the replacement of a valine for glutamate in the β-globin healthy protein. This creates the resultant tetrameric haemoglobin particle to be unstable and the red cells bring this aberrant protein to "sickle," reducing the capacity of these cells to bring oxygen. The ovine β-globin genetics shares 87. 5% similarity with human HBB. We hypothesised that the introduction of the "sickle" mutation in the sheep genome would lead to the SCD phenotype in sheep that might offer an useful platform for assessing postnatal and prenatal drug and gene therapies for this disease. We developed a solitary overview RNA targeting exon 1 of the lamb β-globin/ HBB genetics using the Benchling software. PCR-restriction piece size polymorphism evaluation using Image J software demonstrated a high rate of anomalies by either indels or SCD anomaly that caused the loss of the restriction enzyme site, which was more sustained by the evaluation of cell colonies. These information demonstrate that we efficiently presented the SCD mutation into SFFs. These cells will be made use of in the manufacturing of the first large pet SCD version by somatic cell nuclear transfer in fall of 2020.

Source link: https://pubag.nal.usda.gov/catalog/7229181


Dietary ω-3 Fatty Acid Supplementation Improves Murine Sickle Cell Bone Disease and Reprograms Adipogenesis

In rec H/R SS mice, FD boosts osteoblastogenesis/osteogenic activity by downregulating osteoclast activity using miR205 down-modulation and lowers both systemic and local inflammation. We also reviewed adipogenesis in both AA and SS mice fed with either SD or FD and exposed to rec H/R. Our searchings for provide new insights on the mechanism of action of ω-3 fatty acid supplementation on the pathogenesis of SBD and enhance the rationale for ω-3 fat dietary supplements in SCD as a complementary therapeutic intervention.

Source link: https://pubag.nal.usda.gov/catalog/7386989


Single laser trapping for optical folding and rotation of red blood cells in sickle cell disease in response to hydroxyurea treatment

Optical folding and turning actions of red blood cells in polarized laser tweezers are significantly essential for recognizing the biophysical and biomechanical properties utilizing the rapid probing approach. Folding angle and turning speed are considerably reduced in patients with SCD and do not significantly vary in patients dealt with by HU compared to the healthy and balanced control team. The RBC folding angle and turning speed in patients treated with HU drug boost linearly at lower laser powers and quickly at higher powers, and increase much slowly in patients not treated with HU.

Source link: https://pubag.nal.usda.gov/catalog/7332853


Prevalence of Bartonella spp. Infection in Patients with Sickle Cell Disease

Bartonella spp. A large spectrum of medical manifestations has been connected to bartonella bloodstream infection in the last twenty years that can trigger deadly results, specifically in immunodeficient patients. The goal of this research study was to evaluate the prevalence of bartonella infection in SCD patients. Materials and Methods: We reviewed Bartonella spp. Blood examples and enrichment blood societies were analyzed by molecular detection of Bartonella spp. Bartonella DNA was enhanced utilizing conventional genus-specific Bartonella PCR which magnifies the Intergenic Transcribed Spacer region and Bartonella henselae-specific nested PCR which amplifies the FtsZ genetics. Searchings for: Ten of 107 patients tested positive for B. henselae infection in at the very least one molecular test. Based on statistical outcomes, blood stream infection with B. henselae was not related to pet contact or blood transfusions. Verdict: We detected B. henselae DNA in 10 SCD researched patients.

Source link: https://pubag.nal.usda.gov/catalog/6828459


Smartphone-based sickle cell disease detection and monitoring for point-of-care settings

Optical microscope-based classification and counting needs a significant amount of time, substantial setup, and price along with the knowledgeable human labor to differentiate the typical red cell from sickled cells. In this article, we utilized a smartphone-based picture purchase technique for recording RBC images from the SCD patients in normoxia and hypoxia problems. A computer algorithm is established to set apart RBCs from the patient's blood prior to and after cell sickling. Using the developed smartphone-based technique, we obtained comparable percent of sickle cells in blood samples as evaluated by conventional technique. The industrialized method of testing demonstrates the possible energy of the smartphone-based test for decreasing the total cost of screening and monitoring for SCD, therefore raising the functionality of smartphone-based screening strategy for SCD in low-resource settings.

Source link: https://pubag.nal.usda.gov/catalog/7016079


Co-Inheritance of alpha-thalassemia and sickle cell disease in a cohort of Angolan pediatric patients

The goal of this study was to discover the association in between alpha-thalassemia, fetal hemoglobin, hematological indices, and professional adverse events in Angolan sickle cell disease pediatric patients. An overall of 200 sickle cell disease youngsters were sampled in Luanda and Caxito. The regularity of the 3. 7 kb alpha-thalassemia removal in homozygosity was 12. 5% and in heterozygosity was 55. 0%. An increase in alpha-thalassemia regularity was observed in kids older than 5 years old. For the very first time in Angolan population, the effect of alpha-thalassemia deletion in sickle cell disease was examined and results strengthen that this attribute affects the medical and hematological aspects and generates a milder phenotype.

Source link: https://pubag.nal.usda.gov/catalog/7069787

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions