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In vitro, we wanted to investigate the direct anti-inflammatory function and anti-atherogenic effects of PNLA on activated CD14 monocytes from peripheral blood of patients with rheumatoid arthritis. After lipopolysaccharide stimulation with or without PNLA pre-treatment, flow cytometry was used to determine the proportions of CD14 monocytes expressing TNF-, IL-6, IL-1, and IL-8 in purified monocytes from patients with RA after lipopolysaccharide stimulation. RNA-sequencing investigated the complete transcriptome profile of PNLA-treated, and LPS-activated monocytes from patients with active RA. PNLA inhibited oxidative phosphorylation and mitochondrial dysfunction, according to Canonical Pathway's study, although the sirtuin signaling pathway was opened, which may be incompatible with the pathophysiologic process of atherosclerosis. Conclusion: PNLA has the potential anti-atherogenic and immune-metabolic effects on monocytes that are pathogenic in RA and atherosclerosis. Dietary PNLA supplementation regulates key miRNAs that are key to metabolic, mitochondrial, and inflammation pathways.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/820252
In the meantime, decoy receptor 3 competitively binds soluble LIGHT in comparison to TL1A and FasL, and minimizes the signaling of LIGHT by HEVN and. We hypothesized that LIGHT controls gene expression in RA-FLS. We used to look for genes in which expression in RA-FLS is limited by LIGHT. As non-stimulated control, each sample was incubated with either 1. 0 g/ml recombinant human LIGHT protein or phosphate buffered saline diluted with serum-free Opti-MEM medium as the non-stimulated control. Gene expression in RA-FLS stimulated by LIGHT was compared to that of their respective non-stimulated controls.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/808619
The inflamed rheumatic joint is a highly cellular and complex tissue with rapid recruitment and expansion of several cell types that interact in a variety of ways within a localized area. After tissue homogenization, Rheumatoid arthritis synovium has been mainly studied by immunostaining or by molecular profiling. We present complete spatial RNA-Seq results as a result of cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/794338
Tubuloside B, the key active constituent isolated from Cistanche tubulosa Wight, a traditional Chinese medicine with anti-inflammatory activity, is the key active constituent isolated from Cistanche tubuloside B, which is the most notable component isolated from Cistanche tubulosa Wight, which is a common Chinese medicine with anti-inflammatory activity. The Tub B-treat group was divided into four groups for 24 hours as TNF-B stimulation for 24 hours, the mock group was incubated as normal, and the modeling group was stimulated by TNF- for 24 hours. The Tub B-treat group was divided into four groups by TNF- for 24 hours, the TNF+Tub B-treat group was treated with 60 % Tub B tub B for 24 hours. The Tub B-treat group was treated with 60 m tub B tub B-treat group was divided into tub B-treat group was treated with 60 for 24h, the TNF-B-treat group was stimulated.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/789676
paraphrased arthritis diagnostics using circRNA microarray technology to determine circRNA expression in peripheral blood mononuclear cells of patients with rheumatoid arthritis patients.
Source link: https://www.ncbi.nlm.nih.gov/bioproject/782610
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