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Reverse Transcription Polymerase Chain Reaction Test - Europe PMC

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Last Updated: 07 July 2022

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Clinical significance of chest CT for the exclusion of COVID-19 in pre-admission screening: Is it worthwhile using chest CT with reverse-transcription polymerase chain reaction test?

This review examines the use of chest computed tomography for COVID-19 screening in symptomatic patients. Methods Between April 2020 and March 2021, RT-PCR and chest CT were used to detect COVID-19 in 10 823 symptomatic patients prior to admission. We investigated chest CT during both the COVID-19 pandemic's low- and high-prevalence periods as a reference. In eight patients, respiratory COVID-19 pneumonia was positive. In 36 of 39 patients, a Pneumonia that was labeled as a "typical appearance" of COVID-19 was found false-positives in a "typical appearance" of the disease. Conclusions The addition of chest CT to RT-PCR tests does no help in the detection of COVID-19 in symptomatic patients.

Source link: https://europepmc.org/article/MED/35581125


Comparison of a novel antigen detection test with reverse transcription polymerase chain reaction assay for laboratory diagnosis of SARS-CoV-2 infection.

The gold standard treatment is Molecular diagnosis of severe acute respiratory syndrome coronavirus 2 by real-time reverse transcription polymerase chain reaction in respiratory specimens. RapidForward2122 Antigen Rapid Test Kit is a commercially available Ag-RDT that is manufactured in Turkey and designed to identify SARS-CoV-2's nucleocapsid antigen in nasopharyngeal swab samples. The study included four hundred forty-four nasopharyngeal swab samples from ten centers in Turkey between September 2020 and February 2021, which were collected from the patients who met the clinical criteria of COVID-19. Following the manufacturer's instructions, the RapidFortu2122 SARS-CoV-2 antigen test was used to determine the presence of SARS-CoV-2 antigen in all samples. The prevalence of rapid antigen testing in samples with Ct values above and below 30 percent was 82. 7%, while it climbed to 95. 7% in samples with Ct values below 20 percent.

Source link: https://europepmc.org/article/MED/35513690


Ophthalmology practice in COVID-19 pandemic: Performance of rapid antigen test versus real time-reverse transcription polymerase chain reaction in a tertiary eye care institute in South India.

Objectives: To screen COVID-19 infection in asymptomatic patients undergoing ophthalmic surgical procedures, the quick antigen test against the gold standard reverse transcription-polymerase chain reaction was determined by asymptomatic patients. Following an initial negative RAT, two patients tested positive for SARS-CoV-2 both RAT and RT-PCR, while two others tested positive with RT-PCR. The sensitivity of RAT in comparison to RT-PCR was 33. 3 percent, preciseness was 100%, positive predictive value was 100%, and negative predictive value was 99. 68%. Conclusion and Summary of Our Study The sensitivity and Cohen's kappa coefficient in our study were poor, but this can be attributed to the overall low positivity rates with both RAT and RT-PCR. We therefore recommend starting a questionnaire of all patients for COVID-19 signs and RAT followed by RAT before beginning any ophthalmic surgical procedure to ensure the health care professionals as well as the patients.

Source link: https://europepmc.org/article/MED/35502068

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions