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One RT-PCR target is positive for SARS-Cov-2, and 1 RT-PCR target is negative for SARS-CoV-2 in the same sample, leading to an inconclusive test result. One reason why a sample may produce an inconclusive result is that one target is at a higher level than another one. U2014 To determine the role of subgenomic RNA transcripts in discordant results from RT-PCR experiments for COVID-19. u2014 In culture, infected cells had higher N-gene/ORF1 copy ratios than culture supernatants. In contaminated cells, the same patterns of copies were found in clinical samples, but in infected cells, the more prevalent trend was more apparent. U2014 The following article: Conclusions. u2014 This report found that a greater copy number of N-gene relative to E-gene and ORF1 transcripts could lead to inconsistent results for some RT-PCR tests on low viral load samples, according to this research.
Source link: https://doi.org/10.5858/arpa.2021-0630-sa
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