REPEAT - Europe PMC

A tandem-repeat dimeric RBD protein-based covid-19 vaccine zf2001 protects mice and nonhuman primates.

"We present here the preliminary findings of an approved protein subunit vaccine against COVID-19, ZF2001, which contains tandem-repeat dimeric receptor-binding domain protein with alum-based adjuvant. " In both mice and non-human primates, we evaluated vaccine immunogenicity and efficacy. In both mice and non-human primates, RBD-binding and SARS-CoV-2 neutralizing antibodies were present in both mice and non-human primates, as well as balanced T H 2 cellular responses in NHPs. For NHPs, vaccination of either 25 bcg or 50 % bcg ZF2001 prevented infection with SARS-CoV-2 in lung, trachea, and bronchi, with milder lung lesions. ".

Source link: https://europepmc.org/article/MED/35311493

Application of da Vinci robot and laparoscopy on repeat hepatocellular carcinoma.

"Background" describes the repeated laparoscopic liver resection, which has been used safely and effectively against hepatocellular carcinoma. " However, no research has been done on repeat HCC surgery by a da Vinci robot. This research seeks to determine the results of the patients with repeat HCC treated using a da Vinci robot or laparoscopic device at a single center. Methods In this retrospective review, all of the patients with repeat HCC treated with a da Vinci robotic or laparoscopic device between April 2017 and April 2020 were included. The study included 24 patients with a mean age of 56 years who underwent da Vinci robotic or laparoscopic surgery for treatment of repeat HCC. The normal intraoperative blood loss was 284 mml and 251 ml. The percentages of surgeons who switched to open surgery were 9% and 23%," the surgeons said.

Source link: https://europepmc.org/article/MED/35708383

The Ecology of Palm Genomes: Repeat-associated genome size expansion is constrained by aridity.

"Genome size varies by 2,400-fold across plants, influencing plant evolution by changes in cell size and cell division rates that influence plant's environmental stress tolerance. " However, which environmental stresses that influence repeat community dynamics have yet to be investigated from an ecological perspective. To investigate interactions between repeat dynamics and environmental factors, we created genomic repeat profiles for 141 palm species and analyzed repeats using phylogenetically-informed linear models. Aridity's findings reveal that palm genome size and repeat 'community' composition are best explained by aridity. In arid-adapted palm species, however, elements that might be associated with stress-response genes have increased.

Source link: https://europepmc.org/article/MED/35717562

Designed Ankyrin Repeat Proteins as a tool box for analyzing p63.

"The operation of the p53 transcription factor family is dependent on several folded domains. " We'll now discuss elaborating p63's design Ankyrin Repeat Proteins, which were selected as tight binders against all folded domains of p63. We determine binding affinities as well as specificities within the p53 protein family, and we demonstrate that DARPins can be used as intracellular inhibitors for transcription modification. We show that p53 can be revived by selectively inhibiting DNA binding of the u0394Np63b1 isoform that competes with p53 for the same promoter sites. In addition, the existing DARPins can also be used to specifically identify p63 in cell culture and in primary tissue, making them a very flexible research tool for investigating p63 function.

Source link: https://europepmc.org/article/MED/35717504

Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic Postconditioning Via Modulation of the Akt/Mst1 Pathway Signaling.

"We suspected that the attenuation of myocardial disease by IPostC might be hampered by PHL1 overexpression in diabetic animals. " Methods and findings Nondiabetic and diabetic mice were subjected to 45 minutes of ischemia followed by 2 h of reperfusion with or without IPostC. Cells were exposed to normal or high glucose and were exposed to 4 h of hypoxia followed by 4 h of reoxygenation with or without hypoxic postconditioning. In H9c2 cells exposed to normal glucose but not high glucose, HR-induced cardiomyocyte injury and oxidative stress were tempered by increased p-Akt, decreased PHLPP1 expression, and p-Mst1. In addition, HPostC's combined PHLPP1 knockdown or PHLPP1 knockdown in H9c2 cells exposed to high glucose, which was prevented by a PI3K/Akt inhibitor," according to the authors.

Source link: https://europepmc.org/article/MED/35715527

Recurrent postpartum hemorrhage at subsequent pregnancy in patients with prior uterine artery embolization: angiographic findings and outcomes of repeat embolization.

"Objective": "Objective" is a characterization of angiographic findings and results of a recurrent postpartum hemorrhage in patients with a history of prior UAE. PPH with gelatin sponge slurry in 753 patients between March 2004 and February 2021, respectively. Results The causes of recurrent PPH were found in combination with preserved placental tissue and uterine atony. Both uterine arteries were embolized in the remaining three patients with recanalized or patent uterine arteries. The remaining four patients with obliterated uterine arteries necessitated hysterectomy. Conclusions According to the UAE, permanent uterine artery obliteration occurs due to a gelatin sponge slurry that is often causes permanent uterine artery obliteration. Repeat UAEs may be less likely to have hemostasis in situations of recurrent PPH in subsequent pregnancy. Permanent uterine artery obliteration in the UAE caused by gelatin sponge slurry, which is a frequent cause of permanent uterine artery obliteration. 2 In subsequent pregnancy, the repeat UAE may be less likely to experience hemostasis than the initial UAE.

Source link: https://europepmc.org/article/MED/35671143

MyProstateScore in Men Considering Repeat Biopsy: Validation of a Simple Testing Approach

After a negative biopsy, men with persistent risk of Grade Group u22652 cancer remain a challenge. We wanted to establish a cost-effective MPS-based testing strategy in males with a previous negative biopsy being considered for repeat biopsy. Patients were tested by post-digital rectal examination urine before undergoing repeat biopsy, according to Methods. We found a lower threshold approximating 90% sensitivity and upper threshold approximating 80% sensitivity for GGu22652 cancer in a clinically-appropriate training cohort. On repeat biopsy, 25 men in the 268-patient validation cohort developed GGu22652 cancer. 0% for MyProstateScore 15-40, 5. 5% for MyProstateScore 15-40, and 19% for MyProstateScore > 40 percent. The MyProstateScore scores of 15 and 40 in patients who had previously undergone a negative prostate biopsy revealed clinically-actionable rule-in and rule-out risk groups, according to the authors.

Source link: https://europepmc.org/article/PPR/PPR507620

Survival and Neurodevelopmental Outcomes in Congenital Diaphragmatic Hernia Patients with Single versus Repeat ECMO Runs.

"Objective We discuss the life and neurodevelopmental outcomes of congenital diaphragmatic hernia patients who received single and repeat ECMO" Study design An retrospective single-center study comparing cognitive health in CDH patients treated without ECMO, one ECMO run, and those who underwent two ECMO runs. Second run ECMO patients had increased median ventilator days and an elevated median length of hospital stay in second run ECMO patients. CDH neonates who received ECMO were more likely to have lower cognitive, word, and motor composite scores than those who did not have ECMO. In repeat ECMO run neonates as compared to single ECMO run, however, there were no further deficits identified in language or cognition. In the second run ECMO group, we also see further motor deficits in motor performance as compared to single run ECMO. ".

Source link: https://europepmc.org/article/MED/35709727

Satellite repeat RNA expression in epithelial ovarian cancer associates with a tumor immunosuppressive phenotype.

"In RNA-seq of primary ovarian cancers and associated with reduced survival in a second independent cohort of ovarian cancer patients, the association of HSATII with higher EMT and low IFN response genes was also found in RNA-seq of primary ovarian cancers and was associated with significantly shorter survival. " IFN response and induced MHC I expression in ovarian cancer cell lines was boosted by a novel strategy of modulating the repeatome to re-establish anti-tumor cell immune surveillance, according to the manufacturer.

Source link: https://europepmc.org/article/MED/35708912

The armadillo-repeat containing X-linked protein 3, ARMCX3, is a negative regulator of the browning of adipose tissue associated with obesity.

"Objectives": In the thermogenic plasticity of adipose tissue, armadillo repeat-containing X-linked protein 3 plays a role. Armcx3 gene expression was determined in adipose tissue from mice exposed to thermogenic stimulators and in differentiating brown and beige cells in culture. Armcx3 gene expression was determined in subcutaneous adipose tissue from lean people and obese people, and was positively linked to the expression of marker genes of adipose browning. The effects of adenoviral-mediated overexpression of ARMCX3 on differentiating brown adipocyte gene expression and respiratory function were determined. Male mice that were lacking ARMCX3 had a significant increase in white adipose tissue browning. Experimentally-induced elevations in Armc3 resulted in the down-regulation of thermogenesis-related genes and reduced mitochondrial oxidative activity of adipocytes in culture and lowered mitochondrial oxidative activity of mitochondrial oxidative enzymes, and reduced mitochondrial oxidative capacity of adipocytes in culture, while siRNA-mediated Armcx3 knocked-down increased expression of thermogenesis-related genes.

Source link: https://europepmc.org/article/MED/35705702

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