Advanced searches left 3/3

Pseudogene - PubMed

Summarized by Plex Scholar
Last Updated: 20 July 2022

* If you want to update the article please login/register

Long noncoding RNA (lncRNA) metallothionein 1 J, pseudogene (MT1JP) is downregulated in triple-negative breast cancer and upregulates microRNA-138 (miR-138) to downregulate hypoxia-inducible factor-1α (HIF-1α).

Triple-negative breast cancer is a particularly invasive subtype of breast cancer. By doing real-time quantitative fluorescence PCR, the presence of MT1JP in two types of tissue obtained from 78 TNBC patients was confirmed. MT1JP, miR-138, and HIF-03b1, expression vectors of MT1JP and HIF-1u03b1, as well as miR-138 mimic and inhibitor, were included in BT-549 cells to help analyze the relationship between MT1JP, miR-138 and HIF-1u03b1. In TNBC tissues, the MT1JP was positively linked to miR-138 but was not positively related to HIF-1u03b1. Following the overexpression of MT1JP and miR-138, a decrease in the proliferation rate of TNBC cells was observed. Besides HIF-1u03b1's role in TNBC cell proliferation and migration, the involvement of MT1JP and miR-138 has also been minimized. Our results showed that MT1JP inhibited TNBC by limiting the miR-138/HIF-1-u03b1 axis, implying that MT1JP could act as a biomarker or target for TNBC therapy.

Source link: https://doi.org/10.1080/21655979.2022.2077906


Pseudogene CLEC4GP1 modulates trophoblast cell apoptosis and invasion via IL-15 inhibition.

Healthy placentation is dependent on placental trophoblast cell invasion during early gestation. Thus, trophoblast dysfunction could play a role in PE development. We found C-Type Lectin Domain Family 4 Member G Pseudogene 1, which was aberrantly expressed in PE placental tissues, which was also present in PE placental tissues in the present study. CLEC4GP1 overexpression significantly enhanced cell viability and invasiveness, as well as decreased the apoptosis rate of HTR-8/SVneo and JEG-3 cells, according to in vitro studies, while CLEC4GP1's knockdown had opposite consequences, suggesting the beneficial role of CLEC4GP1 in trophoblast cells. The expression of IL-15 in PE placental tissue has greatly increased in PE placental tissues. In addition, CLEC4GP1 reduces the mRNA stability of IL-16 by inhibiting the binding between human antigen R protein and IL-15 RNA. Finally, the obverse effects of CLEC4GP1 and IL-15 were investigated, and the findings revealed that IL-15 restored cell functions.

Source link: https://doi.org/10.1016/j.yexcr.2022.113215


The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer.

Double homeobox A pseudogene 8 is a well-known tumor promoter in a number of malignancies. DUXAP8 was upregulated in CC, and it was related to advanced stages and lymph node metastases, according to our reports. Based on our Kaplan-Meier survival report, the elevated DUXAP8 expression resulted in shorter patient overall survival. DUXAP8 deficiency strongly stifled vitro's epithelial-mesenchymal transition, according to our western blot report. CC: Alternately, DUXAP8 overexpression accelerated cell proliferation and invasion.

Source link: https://doi.org/10.1080/21655979.2022.2053802

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions