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Protocol - BioProject

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Last Updated: 24 May 2022

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Development and optimization of a silica column-based extraction protocol for ancient DNA

We investigated the accuracy of pretreatment of bone and tooth powder with a bleach wash and a predigestion step using relatively well-preserved permafrost samples. We found that neither bleach washing mixed with a predigestion step nor predigestion by itself increased sequencing effectiveness. Initial findings, however, indicate that MinElute columns are more effective for ancient DNA extractions than QIAquick columns, than QIAquick columns, although different molecular weight cut-off values in centrifugal concentrator columns did not have a role. And at concentrations of 0. 15 U/L of ancient DNA extracts, Uracil drugs are very effective at removing DNA damage.

Source link: https://www.ncbi.nlm.nih.gov/bioproject/840416

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions