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Protein - Astrophysics Data System

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Last Updated: 24 July 2022

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ProteinKG65: A Knowledge Graph for Protein Science

Unfortunately, existing data-centric methods for protein research can't adequately capture and utilize biology knowledge, which may be essential for several protein tasks. ProteinKG65 is mainly dedicated to providing a specialized protein knowledge graph, bringing the ability of Gene Ontology to protein function and structure prediction.

Source link: https://ui.adsabs.harvard.edu/abs/2022arXiv220710080C/abstract


Analysis of carbohydrate, protein and fat levels using various type rice with different cooking process

The results of this analysis revealed that the highest carbohydrate content is found in raw white rice at 126. 124 mg/kg, while after the cooking process that the highest carbohydrate levels were discovered in white rice used ricecooker at 103. 400 mg/kg. The highest protein levels before processing were found in raw black rice at 11. 1 percent, while white rice at 8. 7 percent, according to the highest protein levels found in black rice at 5. 12 percent, while the lowest was found in steamed black rice at 3. 9 percent.

Source link: https://ui.adsabs.harvard.edu/abs/2022E&ES..977a2079C/abstract


(Digital Presentation) Design and Development of Electrochemical Immunosensor for Brain-Related Protein

The process of diagnosing neurodegenerative diseases depends on the onset of symptoms. ALS is a neurodegenerative disease that affects the motor system. Individuals suffer a loss of mobility in their appendages and limbs, which leads to complete muscle paralysis as the disease progresses. Early detection of ALS can be aided by detecting biomarkers associated with the disease, one of which is the TDP-43 protein. Before and after protein exposure, the antibody-surface's charge transfer resistance was evident, as shown by the signal strength. TDP-43 antibodies were added to the surface using a gold disk working electrode, which underwent surface modification, to synthesize TDP-43 antibodies. Rct values were seen above 100 nM concentrations, indicating dramatic changes in Rct values. This review details the process for manufacturing a sensor recognition layer for TDP-43 specifically, but it can also be expanded to include other disease-related biomarkers.

Source link: https://ui.adsabs.harvard.edu/abs/2022ECSMA2022.2479T/abstract


Applications in Stimulated Emission Depletion Microscopy: Localization of a Protein Toxin in the Endoplasmic Reticulum Following Retrograde Transport

Retrograde transport is a process in which proteins are transported from the plasma membrane and endosomes to biosynthetic and secretory organelles, namely the Golgi apparatus and endoplasmic reticulum. Here we demonstrate how to visualize exogenously applied ricin toxin within the ER with the help of super-resolution and live-cell imaging [stimulated emission depletion ].

Source link: https://ui.adsabs.harvard.edu/abs/2016MiMic..22.1113H/abstract


Structural dynamics of light harvesting proteins, photosynthetic membranes, and cells observed by spectral editing solid-state NMR

This short article discusses the use of magic angle spinning nuclear magnetic resonance spectroscopy for determining the morphology of pigment, protein, and thylakoid membrane components related to light harvesting and photoprotection. Dynamic spectral editing NMR has been used to investigate light-harvesting complex II protein conformational dynamics inside lipid bilayers and in native membranes. NMR can be used to reduce spectral complexity by filtering motion-dependent signals, enabling us to track processes in living photosynthetic cells, according to this article.

Source link: https://ui.adsabs.harvard.edu/abs/2022JChPh.157b5101P/abstract


Protein corona of SiO 2 nanoparticles with grafted thermoresponsive copolymers: Calorimetric insights on factors affecting entropy vs. enthalpy-driven associations

The titrant concentration was an estimate, restricting the accuracy of thermodynamic measurements. Positive u03be-potentials related to the blood proteins were found in the particles with grafted polymer brushes, and their interactions against Coulomb forces were similar to the blood proteins. Two instances of exothermic hydrophobic and endothermic polar interactions were distinguishable. The adsorption of proteins from blood plasma was always exothermic, and, consequently, the dominant hydrophobic interactions were assumed.

Source link: https://ui.adsabs.harvard.edu/abs/2022ApSS..60154201N/abstract


Application of Genetically Encoded Photoconvertible Protein SAASoti for the Study of Enzyme Activity in a Single Live Cell by Fluorescence Correlation Microscopy

Capase enzymatic activity in a single live cell is based on the principle of using photoconvertible proteins to determine caspase enzymatic activity in a single live cell. This FRET sensor has a predictable sequence in the linker between two FPs for the finding of one of the key enzymes of apoptosis, caspase-3. Caspase-3's activity was detected by monitoring the increase in the sensor's fluorescent lifetimes, although SAASoti's diffusion coefficient decreased. Cell structure has already shown significant changes in viscosity at the time of detectable caspase-3 activity. We can assume that in the earliest stages of detectable caspase-3 activity, cell membrane structure has already made important changes in viscosity.

Source link: https://ui.adsabs.harvard.edu/abs/2022Mate...15.4962S/abstract

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions