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For the first time DNA methylation in the foci of 5S and 35S rDNA sequences in individual Brachypodium distachyon micronuclei that are caused by mutationagenic therapy with maleic acid hydrazide is shown in this paper. Prioritizing MH in plants has been investigated, but no in situ analyses of DNA methylation in specific DNA sequence sites are known; however, no in situ analyses of DNA methylation in nuclei and micronuclei have been reported. In plant mutagenesis, such studies into the presence or absence of DNA methylation within particular DNA sequences are particularly important in plant mutagenesis, particularly because gene expression can change.
Source link: https://europepmc.org/article/MED/35743241
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