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Mutagen - Crossref

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Last Updated: 13 November 2022

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Induksi Keragaman Genetik pada Tanaman Alocasia Menggunakan Mutagen Kimia Kolkisin

Alocasia green velvet is one of the ornamental plants that plant enthusiasts hunt for. This gives ornamental plant farmers the opportunity to showcase the potential of Alocasia. Genetic diversity in Alocasia is extremely low because, generally these plants are propagated as vegetative, which is very poor. In order to ensure that new Alocasia species are produced with more appealing leaf shapes, sizes, and colors, it is imperative to increase genetic diversity. The tuber of the Alocasia plant was used to make the plant material. A different leaf shape was obtained after a Colchicine treatment of 10 mgL-1 with immersion time of 1 day. The Colchicine treatments of 10, 15, 20, and 25 mgL-1 produced plants with more leaf coloration than the control. As well as the 30 mgL-1 colchicine cure, vigorous plants with larger stems were produced.

Source link: https://doi.org/10.33394/bjib.v9i1.3753


Effect of Chemical Mutagen on Yield and Yield Attributing Traits in Chickpea (Cicer arietinum L.)

An experimental trail was run to determine the effect of sodium azide on yield and its properties of chickpea. In the M1 generation of chickpea, sodium azide with differing concentrations on yield and its attributing characteristics was investigated. Whereas the effect of sodium azide on the average mean of quantitative traits on 03 chickpea genotypes is unclear, plant height is the most significant, while attributing characteristics are not.

Source link: https://doi.org/10.9734/ijpss/2021/v33i1830588


Proteome Profiling of the Mutagen-Induced Morphological and Yield Macro-Mutant Lines of Nigella sativa L.

The leaf proteome profile of Nigella sativa L. 's macro-mutant lines was analyzed to determine the key proteins involved in the expression of attributes related to the morphology, seed yield, and thymoquinone content was determined in this research. Twenty-three proteins were differentially expressed in the leaf proteome of N. sativa's control and macro-mutant lines, demonstrating that twenty-three proteins were differentially expressed. Proteins from secondary plant metabolism, stress defense, cytoskeleton, and protein synthesis were also discovered. Expression profiles of the differentially expressed proteins were also investigated at the transcript level by using qRT-PCR. This paper explores the mutations that occur at the proteomic level during mutagenesis induced mutation, as well as the involvement of certain proteins in the expression traits associated with plant height, seed yield, and N. sativa's thymoquinone content.

Source link: https://doi.org/10.3390/plants8090321


Mutagen sensitivity and risk of second cancer in younger adults with head and neck squamous cell cancer: 15-year results

Abstract Purpose: The aim of this paper is to investigate the mutagen sensitivity phenotype in patients with head and neck squamous cell carcinoma, as well as the SPC's long-term incidence and the event with SPC. Methods An examination was conducted into SPC among 124 younger adults with HNSCC who were participating in a pretreatment mutagen sensitivity investigation during 1996–2006. In vitro, exposing lymphocytes to bleomycin in vitro and measuring the bleomycin-induced chromatid breaks per cell were determined by Mutagen sensitivity, according to Mutagen. According to 15 and 17%, respectively, the crude rate of SPC for hypersensitive or non-sensitive patients was between 15 and 17%. According to the latter, the 15-year estimated incidence of SPC in hypersensitive and not hypersensitive patients was 36 and 48 percent, respectively. Conclusion According to our results, mutagen hypersensitivity was not related to an elevated SPC risk in HNSCC patients.

Source link: https://doi.org/10.1007/s00066-022-01917-2


Hydrogen peroxide induces DNA single- and double-strand breaks in thyroid cells and is therefore a potential mutagen for this organ

In this study, we investigated whether the elevated levels of H2O 2 found in the thyroid to oxidize iodide could cause DNA changes. We found that non-lethal H 2 O 2 deposition in a rat thyroid cell line as well as irradiation caused a significant number of SSBs but also high amounts of DSBs. Moreover, we found that DNA breaks induced by H 2 O 2 were more easily repaired than those caused by radiation. In conclusion, H 2 O 2 leads to SSBs and DSBs in thyroid cells. These results point to the fact that H2 O 2 production in thyroid might have also played a role in mutagenesis, particularly in the case of antioxidant defense deficiency.

Source link: https://doi.org/10.1677/erc-09-0020


Abstract 3469: Effect of age and mutagen on the enriched murine mammary epithelial stem and progenitor cell population

Nonetheless, there is insufficient information on the mechanisms that influence breast cancer initiation and promotion in older women. Functional mammary outgrowths indicating stem cell function are unaffected by the donor's age have been documented earlier by serial transplantation of murine mammary tissues. Although the frequency of mammosphere development remained the same, the number of larger mammospheres from old mice was significantly lower than those from young mice, possibly indicating reduced stem cell production in the older population. We treated two young and two old mice with methyl nitrosourea once a week for three weeks in vivo to investigate mutagen in vivo. Both total and larger mammospheres from the treated older mice's isolated Linu2212MEC were found in significantly higher numbers than the treated younger mice. In a recent clinical study, larger mammospheres isolated from metastatic breast cancer cells have been correlated to tumor formation. These Linu2212MEC cells were also tested and compared for DNA damage by alkaline comet assay, and a higher percentage of cells with irreversible DNA damage in the elderly population was found compared to the younger population, indicating potential lower DNA repair capacity of the former cells. Our preliminary findings revealed that enriched stem/progenitor cells in the old mice with reduced in vitro stem cell proliferation may be susceptible to DNA damage caused by mutagens and subsequent tumorigenesis.

Source link: https://doi.org/10.1158/1538-7445.am10-3469


Abstract 4118: Hypoxia affects the metabolic activation and detoxification of the environmental mutagen benzo(a)pyrene

Since proper carcinogen metabolism is dependent on oxygen, we investigated how changing oxygen levels affected BaP metabolism by investigating its activation and deactivation. During 18 hours, BaP was administered to the human lung carcinoma cell line A549 under different oxygen concentrations for 18 hours, and BaP metabolism changes were determined. Under hypoxia, expression of key metabolic enzymes in BaP was elevated; expression of CYP1A1 and CYP1B1 were also elevated; expression of the detoxifying enzymes UGT1A6 and UGT2B7 was dramatically reduced by hypoxia. By HPLC-fluorescence analysis, the number of BaP metabolites in supernatants were identified. Then, several BaP metabolites in supernatants were identified by HPLC-fluorescence analysis. BaP metabolism is slower as a result of poor oxygen levels, but more of the parent molecule remains unchanged. Eventually, under hypoxia-specific conditions, DNA adducts were accumulated over a period of 168 hours, whereas DNA adducts were effectively deleted under 20% oxygen, resulting in 1. 6 times higher adduct levels under hypoxic conditions.

Source link: https://doi.org/10.1158/1538-7445.am2012-4118


Abstract 3718: Well-done meat intake and meat-derived mutagen exposures in relation to breast cancer risk: The Nashville Breast Health Study

Abstract: Previous studies of the relationship between meat intake and meat-derived mutagen exposure to breast cancer risk have varied findings. We reviewed this association in a population-based case-control review of incident breast cancer research in Nashville, Tennessee, United States, which involved 2,386 breast cancer cases and 1,703 healthy women controls. Telephone interviews were conducted to collect evidence related to meat intake, including amounts, cooking techniques, and dopeiness, as well as other commonly used or hypothesized risk factors for breast cancer. Breast cancer risk was greatly raised if you consumed red meat. The link between red meat and breast cancer risk was greater among postmenopausal women than for premenopausal women. The results from this research show that elevated red meat intake and meat-derived mutagen exposure may be associated with an increase in breast cancer risk.

Source link: https://doi.org/10.1158/1538-7445.am2011-3718


Abstract P3-08-01: Gamma-ray induced mutagen sensitivity and overall survival in young women with breast cancer

Abstract Background: Hypersensitivity to radiation has been shown to be a risk factor for breast cancer formation. We'll explore whether the same hypersensitivity predicts adverse clinical outcomes in patients with breast cancer. Methods: This analysis included 465 young, female, non-Hispanic white patients with carcinoma of the breast at our hospital from 1/1997 to 12/2005. Each single chromatid break was counted as a single break, exchange figure, or interstitial deletion as two breaks, with each chromatid break counted as a single break. The mean value of chromatid breaks per cell was then determined and recorded and reported. To determine the hazard ratio and 95% confidence interval for the relationship between b/c and overall survival, the Cox multivariable proportional hazards model was used to estimate the hazard ratio and 95% confidence interval. 341 patients had invasive cancer, and 253 patients had ER+ disease. stage 0, stage 1, stage 2, stage 3, and stage 4. AJCC stage presentation was stage 0, stage 1, stage 2, stage 2, stage 3, and stage 4. In patients with b/c greater than the median value of 0. 5 percent, a statistically significant decrease in 5 and 10-year overall survival was seen in patients with b/c greater than the median value of 0. 5. Conclusions: In this cohort of young, female, non-Hispanic white breast cancer patients, there was a rise in the b/c ratio, which contributed to reduced overall survival. The use of a gamma-ray-triggered mutagen detection kit assay may be prognostic and aid in the selection of people at an elevated risk of death. In:: Proceedings of the Thirty-Seventh Annual CTRC-AACR San Antonio Breast Cancer Symposium, 2014 Dec 9-13; San Antonio, Texas.

Source link: https://doi.org/10.1158/1538-7445.sabcs14-p3-08-01

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions