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Mrna - Europe PMC

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Last Updated: 07 June 2022

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Spelling out the roles of individual nucleoporins in nuclear export of mRNA.

"The Nuclear Pore Complex is a critical passage through the nuclear envelope for nuclear import and export that has a direct effect on virtually every cellular process at some degree. ".

Source link: https://europepmc.org/article/MED/35593254


Identification of key genes and miRNA-mRNA regulatory networks associated with bone marrow immune microenvironment regulations in multiple myeloma by integrative bioinformatics analysis.

Multiple myeloma is a result of the deregulation of microRNAs and genes in the bone marrow microenvironment, which has been implicated in multiple myeloma pathogenesis. " We identified 112 hub genes associated with five major clusters in MM, which were mapped to five specific clusters. In addition, we discovered 9 upregulated hub genes and 52 downregulated hub genes in MM that is specifically targeted by DEmiRs. MM patients' survival prognosis is correlated with the expression of DEmiRs targeting two hub genes. In addition, the expression level of CDKN2A in the TME of MM is related to immune signatures, including CD4+ regulatory T cells, T cell exhaustion, MHC Class I, immune checkpoint genes, macrophages, neutrophils, and TH2 cells. Identifying key genes and miRNA-mRNA regulatory networks may provide new molecular insight into the tumor immune microenvironment in MM. ".

Source link: https://europepmc.org/article/MED/35536760


Transcriptomic analysis of the innate immune signatures of a SARS-CoV-2 protein subunit vaccine ZF2001 and an mRNA vaccine RRV.

"Analysis of large-scale gene expression post vaccination will give an overview of immune responses. " We used transcriptional techniques to comprehensively investigate the innate immune response signatures produced by protein subunit vaccination ZF2001 and an mRNA vaccine named RRV. ZF001-induced MHC class II-related genes, including cd74 and H2-Aa, in a more efficient way than the RRV, according to a fine-grained time-dependent dissection of large-scale gene expression post immunization. The antibodies of binding and neutralization antibodies produced by both vaccines were similar on day 21 post immunization, and B/T cells activation were similar, according to the expression level of genes essential to BCR/TCR signalling transduction and B/T cells activation at day 7. Generally, the transcriptomic profiles of two very different vaccines mapped here provide a framework for determining correlations between gene expression and protection, which can be used to determine specific and robust immune responses against SARS-CoV-2. ".

Source link: https://europepmc.org/article/MED/35343384


Effects of vitamin C and D on the mRNA expression of angiotensin converting enzyme 2 receptor, cathepsin L, and transmembrane serine protease in the mouse lungs.

"The SAR-CoV-2 virus attaches to angiotensin converting enzyme 2 receptor and the proteolytic activity of Cathepsin L and transmembrane serine protease 2 enzymes in the pulmonary endothelial cells," the SAR-CoV-2 virus integrates into the pulmonary endothelial cells. " This research was done to determine the influence of Vit C and D on the mRNA expression of Ace2, Tmprss2, and Ctsl genes in mouse lungs. Vit D, but not C, upregulated Ace2's mRNA expression by more than six folds, while Ctsl and Tmprss2 genes were downregulated by 2. 8 and 2. 2 folds, respectively. ".

Source link: https://europepmc.org/article/MED/35311495


CRC-derived exosomes containing the RNA binding protein HuR promote lung cell proliferation by stabilizing c-Myc mRNA.

"The exosomal HuR's role in colon cancer lung metastasis is uncertain. " HuR expression was determined immunohistochemically in tumor tissue samples from 20 patients with metastatic or nonmetastatic colon cancer and colon cancer lung metastasis, as well as benign lung disease samples from ten patients. Using fluorescence and confocal microscopy, Exosomes were isolated from HCT116 WT and HuR KO colon cancer cells, and BEAS-2B cells' uptake of PKH67- and PKH26-labeled exosomes was examined by BEAS-2B cells. HuR overexpression in lung tissues of patients with lung metastasis in clinical patients was significantly raised in colon tissues. HuR expression in lung tissue with metastasis of colonic origin was higher than that of benign lung disease. HuR overexpression has been linked to lung metastasis in colon cancer patients. The exosomal HuR derived from colon cancer cells alters the biological reaction of normal lung epithelial cells.

Source link: https://europepmc.org/article/MED/35130122


COVID-19 mRNA Vaccine Induced Pericarditis With Large Pericardial Effusion Followed by New-Onset Arrhythmia

"After receiving mRNA COVID-19 vaccination, there have been many cases of myocarditis and pericarditis. " Here we present an example of a middle-aged Caucasian male who developed progressive dyspnea, fever, and chills seven days after receiving a second dose of the COVID-19 vaccine. A correlation exists between mRNA COVID-19 vaccine and pericarditis, according to our case study, which highlights the importance of pharmacovigilance and reporting adverse events, as well as a high incidence of clinical suspicion in physicians that aids early diagnosis and intervention. ".

Source link: https://europepmc.org/article/MED/PMC9162096


Development and application of an uncapped mRNA platform

"Quantified target protein expression was established in vitro and in vivo with eGFP- and Fluc-mRNA-LNP. " Both humoral and cellular immune responses were similar to that observed previously in capped mRNA-LNP constructs, according to Su03b4T-mRNA-LNP, gDED-LNP, and gDFR-mRNA-LNP. Notably, 25, 50, 100, and 100 bcg of S-u03b4T-mRNA-LNP elicited neutralizing antibodies in hamsters against the Omicron and Delta strains. In the in vivo expression of luciferase and antibody generation by Snu03b4T-LNP, mRNA constructs with uridine triphosphate outperformed those with N1-methylpseudouridine triphosphate and antibody production using S-u03b4T-LNP. Our uncapped, process-simplified, and cost-effective mRNA platform may have broader uses in vaccines and protein replacement drugs. ".

Source link: https://europepmc.org/article/PPR/PPR502625


The evolution of pre-mRNA splicing and its machinery revealed by reduced extremophilic red algae.

In C. merolae, in comparison to G. sulphuraria, Introns are unusual and spliceosomal machinery is drastically reduced," says the author. Divergent spliceosomes were also found in the mesophilic red alga and the red algal derived plastid of Guillardia theta, as well as unusually high levels of unspliced transcripts, according to previous research. To investigate red algae splicing evolution, we compared C. merolae and G. sulphuraria, intron position, intron sequence characteristics, and the spliceosome's composition. In addition to identifying 11 new introns in C. merolae, our transcriptomic analysis in G. sulphuraria revealed typical eukaryotic splicing in G. sulphuraria, whereas most transcripts in C. merolae remain unspliced. The occurrence of intron positions within their host genes was investigated in order to provide insight into red algae's intron loss patterns.

Source link: https://europepmc.org/article/MED/35662328


Renal Megalin mRNA Downregulation Is Associated with CKD Progression in IgA Nephropathy.

"Patients in the highest tertile of renal megalin mRNA had a reduced risk of CKD progression than those in the lowest tertile. " The addition of renal megalin mRNA to a model with standard risk factors increased risk prediction of disease progression. Moreover, patients in the highest tertile of renal miR-148b were at 2. 3 percent higher risk of CKD progression compared to those in the lowest tertile. Conclusions Lower renal megalin mRNA levels were correlated with an elevated risk of CKD progression in IgAN, regardless of clinical and pathological causes, indicating that renal megalin may be a significant prognostic factor for IgAN. ".

Source link: https://europepmc.org/article/MED/35661648

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions