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Microbial Source Tracking - DOAJ

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Last Updated: 15 June 2022

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An Overview of Microbial Source Tracking Using Host-Specific Genetic Markers to Identify Origins of Fecal Contamination in Different Water Environments

"Notably, bacterial source identification is widely used to determine the host source of fecal water pollution by the identification of zoogenic and/or anthropogenic sources of fecal environmental DNA. This paper is intended to provide the most up-to-date overview of gene marker-based MST research carried out in different water types, including freshwater and seawater, as well as drinking water systems. This research, focusing on the latest scientific achievements/achievements, aims to gain more insight into the applicability and robustness of using MST for water quality surveillance.

Source link: https://doi.org/10.3390/w14111809


Assessment and application of host-specific Bacteroidales genetic markers for microbial source tracking of river water in Japan.

"Microbial source monitoring using host-specific microbial genetic markers is regarded as a promising way to discover fecal contamination sources of aquatic environments. " This research sought to determine the application of previously developed host-specific Bacteroidales quantitative PCR assays to river water samples analysis in Yamanashi Prefecture, Japan. These selected assays were used for microbial source monitoring of 63 river water samples collected at nine locations in two river basins. These assays established the effects of human and animals' fecal contamination of river water.

Source link: https://doi.org/10.1371/journal.pone.0207727


Synergy between quantitative microbial source tracking (qMST) and quantitative microbial risk assessment (QMRA): A review and prospectus

"The use of microbial source tracking marker genes has increased in recent years in recent years due to the need to assign point and non-point fecal contamination to specific sources. " A modeling technique used to assess health risks from exposure to feces-contaminated water and related pathogens is quantitative microbial risk assessment. The purpose of the reviewed studies was mainly intended to determine what MST marker genes in hypothetical recreational waterbodies would exceed the United States Environmental Protection Agency risk benchmarks for primary contact recreators. Based on published findings in the literature, QMRA models calculated relationships between MST marker gene and reference pathogens. This metric needs further data to establish the correlation between MST marker genes and the degree of variation in pathogen decay as a result of environmental conditions and mixtures of fecal sources at various spatial and temporal scales. ".

Source link: https://doi.org/10.1016/j.envint.2019.03.051


PCR data and comparative performance of Bacteroidales microbial source tracking genetic markers

"We reported modified endpoint PCR results obtained by universal and human-, swine-, and cattle-specific Bacteroidales gene markers with human sewage and animal fecal samples from Tha Chin and Chao Phraya watersheds. " Environ, Somnark et al. , 2006 results analysis of Bacteroidales genetic markers for human and animal microbial source monitoring in tropical agricultural watersheds.

Source link: https://doi.org/10.1016/j.dib.2018.04.129


Human Health Risks Associated with Recreational Waters: Preliminary Approach of Integrating Quantitative Microbial Risk Assessment with Microbial Source Tracking

Using quantitative risk assessment, quantitative microbial risk estimation was estimated for gastrointestinal disease risks associated with exposure to waters affected by human and nonhuman fecal sources. The risk of GI illness was determined for a small sampling site with a geometric mean of E. coli units of 163 colony colonies 100 mL u22121 u22121, and the national standard of E. coli, 126 mL u22121. Though the majority of common sources of fecal indicator bacteria at the sampling site were nonhuman, the risk of disease from norovirus, the reference pathogen expressing human waste, was the greatest threat to human health. ".

Source link: https://doi.org/10.3390/w12020327


Microbial Source Tracking in a Watershed Dominated by Swine

"The high rate of swine production in southeastern North Carolina has raised public health questions regarding the potential transfer of pathogens from these production units to nearby surface waters. " According to an average of 64% and 27. 1% of 1,961 isolates from Six Runs Creek were contaminated with lagoon effluent and cattle manure respectively, comparisons of isolates from water samples to an ARA library with an average rate of correct classification of 94 percent indicated an average of 64% and 27. 1%.

Source link: https://doi.org/10.3390/w2030587


Use of metagenomic microbial source tracking to investigate the source of a foodborne outbreak of cryptosporidiosis

"Cryptosporidium, a protozoan parasite of global public health importance that causes gastroenteritis in a variety of vertebrate hosts, with several human outbreaks reporting every year, many from ingestion of contaminated water or food. " To do so, we combined traditional outbreak investigation techniques with molecular detection and characterization of romaine lettuce samples obtained at the same farm from which the contaminated food was obtained to make it possible. To do so, we combined traditional outbreak investigation techniques with molecular detection and characterization techniques. We found C. parvum in two out of three lettuce samples, which was confirmed by rRNA analysis findings. The lettuce samples revealed sewage water as a possible source of the contamination, but with some confusion. "In addition, the high degree of overlap in bacterial species composition with a public human gut microbial database corroborated the source tracking findings. ".

Source link: https://doi.org/10.1016/j.fawpar.2021.e00142

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions