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The sample surface of the Singapore Synchrotron Light Source's LiMiNT micro/nanomanufacturing plant is about 2 kev to ten keV, providing significant photon energy to perform deep x-ray lithography. x-rays penetrate through resist materials with little scattering in this energy range. The x-ray source's highly collimated rectangular beam architecture allows for a full 4′′ wafer scale fabrication. Precise control of dose deposited controls are precise, allowing 1800 discrete gray levels in a chip of area 20 mm2 and more than 2000 within our reach. Mass manufacturing is especially important if these devices are to be exported in large quantities, particularly as components for original equipment manufacturers, as has been demonstrated by our research.
Source link: https://europepmc.org/article/MED/35177735
Fibroblast cell migration plays a significant role in the wound-healing process. The wound-healing process can be carried out by a microfluidic-based wound-healing assay. This work demonstrated the use of a microfluidics unit to simulate traumatic wounds on fibroblast cell monolayers by using trypsin flow and PDMS barrier. Cell culture conditions mimic a 3D extracellular matrix like environment and cell culture monitoring is real-time. In the presence of optimal shear stress, wound healing was faster wound healing was achieved at the end of 24 hours of the wound's creation in the absence of optimal shear stress. It dissociates fibroblast cells from the substrate's surface, slowing the wound-healing process as increasing the shear stress above a threshold threshold. Both coplanar microfluidics surfaces and transitional microfluidics channels were altered, indicating that the above phenomena were replicated.
Source link: https://europepmc.org/article/MED/35208429
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