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MicroRNA Biogenesis - DOAJ

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Last Updated: 06 February 2022

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MicroRNA biogenesis and activity in plant cell dedifferentiation stimulated by cell wall removal

Conclusions In this study, we investigated the role of miRNAs in the dedifferentiation of A. thaliana mesophyll cells in a process aided by the cell wall's enzymatic removal of the cell wall. Then analysis of differentially expressed miRNAs, selected pri-miRNA, and mRNA samples were discovered in protoplasts rather than in CDPs cultured for 120 hrs. Conclusion In the initial stages of dedifferentiation within CDPs divisions, miRNA function is not a key regulator of gene expression, but in later steps of dedifferentiation during CDPs divisions, this finding indicates that miRNA function is not a key regulator of gene expression in the initial, but not so much in later steps of dedifferentiation. The increased mortality and reduced cell division of CDPs derived from mutants with ineffective miRNA biogenesis and miR319b expression demonstrated the crucial role played by miRNAs in the process of dedifferentiation of mesophyll cells, which was confirmed by the increased mortality and decreased cell division of CDPs derived from mutants with impaired miRNA biogenesis and miR319b expression.

Source link: https://doi.org/10.1186/s12870-021-03323-9


Disruption of microRNA biogenesis confers resistance to ER stress-induced cell death upstream of the mitochondrion.

Several aspects of the miRNA biogenesis machine have been shown to function as haploinsufficient tumor suppressors. How the deregulation of miRNA biogenesis promotes tumor formation is not fully understood. We discovered that HCT116 cells with a DICER hypomorphic mutation, or where DICER or DROSHA were knocked down were resistant to ER stress-induced cell death, as shown by the following table. On ER stress therapy, the extensive research revealed no difference in the unfolded protein response of WT compared to Exn5/Exn5 HCT116 cells. According to this, disrupted miRNA biogenesis may lead to cancer progression by reducing ER stress-induced cell death.

Source link: https://doi.org/10.1371/journal.pone.0073870


MapToCleave: High-throughput profiling of microRNA biogenesis in living cells

MiRNA precursors with a stable lower basal stem are more effective processed and have elevated expression in vivo in tissues from 20 animal species. MiRNA precursors from ten animal and plant species in human cells are systematically assessed, ranging from the importance of a well-known and novel sequence, chemical structure, and test biogenesis of miRNA precursors. In summary, we use a screening assay in living cells to reveal the importance of lower basal stem stability for miRNA processing and in vivo expression.

Source link: https://doi.org/10.1016/j.celrep.2021.110015


In-Depth Analysis of the Interaction of HIV-1 with Cellular microRNA Biogenesis and Effector Mechanisms

To unequivocally state that HIV-1 does not encode any viral miRNAs, we first used deep sequencing of small RNAs present in two separate infected cell lines and two forms of primary human cells. Perhaps more surprising, we also found that HIV-1 infection of T cells has only had a modest effect on cellular miRNA expression at early times after infection. HIV-1 has been described as either expressing viral miRNAs or dramatically altering the level of cellular miRNA expression, according to previous studies, HIV-1 can subvert the function of the cell miRNA machinery by expressing viral miRNAs or dramatically altering the rate of cellular miRNA expression. These results show that HIV-1, rather than trying to monitor miRNA function in infected cells, has instead developed a mechanism to become largely invisible to cellular miRNA effector mechanisms.

Source link: https://doi.org/10.1128/mBio.00193-13

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions