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Asthma is a chronic pulmonary inflammatory disease with persistent pulmonary inflammation. Methods Quantitative real-time polymerase chain reaction was used to determine the expression of miR-629-3p and forkhead box a2 mRNA in 16HBE cells treated with interleukin-13. Through enzyme-linked immunosorbent assay, the amounts of C-C motif chemokine ligand 11, C-C motif ligand 26, interleukin-1 beta, and interleukin 6 were determined in 16HBE cell supernatant, including interleukin-1 beta and interleukin-6. Besides, the target relationship between miR-629-3p and FOXA2 mRNA 3'-UTR was established by dual-luciferase reporter gene assay. In 16HBE cells, the use of FOXA2 protein was determined by Western blot. Results MiR-629-3p expression was significantly enhanced in IL-13-stimulated 16HBE cells, but the FOXA2 mRNA and protein levels were significantly down-regulated.
Source link: https://doi.org/10.1007/s12013-022-01072-6
Hypoxic-ischemic brain damage is a common medical condition. MiR-192-5p in HIBD is found by regulation of the Yes-associated protein 1-mediated Hippo signaling pathway, according to the scope of this research. The regulatory connection between miR-192-5p and YAP1 was confirmed. In vivo and OGD neurons in vitro, MiR-192-5p expression was increased, but YAP1 expression was reduced in hippocampal tissues of HIBD rats in vivo and OGD neurons in vitro, while YAP1 levels were reduced in hippocampal tissues of HIBD rats. In vivo, suppressed miR-192-5p or overexpressed YAP1 in HIBD rats reduced neurobehavioral and neuronal injury, and neuronal damage, and reduced the expression of p-TAZ and VEGF in vivo. In vitro, graduated miR-192-5p or augmented YAP1 decelerated the neuron apoptosis, decreased the p-TAZ level and VEGF level, and boosted cell viability of OGD hippocampal neurons in vitro. According to the report, miR-192-5p protects against HIBD through the regulation of the YAP1 and Hippo signaling pathway, which is helpful in HIBD therapy.
Source link: https://doi.org/10.1007/s11064-021-03518-4
This study was intended to determine the role of long-coding RNA rhabdomyosarcoma 2 related transcript in IS by microRNA-221-3p/phosphoinositide-3-kinase regulatory subunit 1 / increasing growth factor-axis. In middle cerebral artery occlusion mice, neurological function, pathological changes in brain tissue, oxidative stress, and inflammation responses were all assessed. RMST, miR-221-3p, PIK3R1, and TGF- signaling-related protein expression in brain tissues of MCAO mice was found in brain tissue samples. With MCAO, depleted PIK3R1 or restored miR-221-3p offsets the negative effects of overexpressed RMST on mice. The present study demonstrates that RMST improves IS by reducing miR-221-3p-mediated control of PIK3R1 and boosting the TGF- pathway, increasing the TGF- pathway.
Source link: https://doi.org/10.1007/s12035-021-02632-2
This article explores the protective and therapeutic effects of miR-22 on neurological disorders and injuries, including cerebral ischemia, neurodegenerative disorders, epilepsy, and brain malignancies. We also linked miR-22 with amyotrophic lateral sclerosis, multiple sclerosis, anxiety disorders, schizophrenia, neural tube defect, and traumatic brain injury. This paper provides a therapeutic perspective on miR-22 as a new strategy in treating neurological disorders.
Source link: https://doi.org/10.1007/s12035-022-02769-8
The ability of the hybridization reaction between the capture probe and the target could be used to convert insoluble precipitates into the nanochannels by the detection interface. A porous carbon nanofibers-modified electrode, Then, a porous carbon nanofibers-modified electrode, was used to raise the sensing interface's sensitivity to the sensor's response to the concentration change of the redox probe methylene blue. MicroRNA samples in serum and tumor cells can be determined in a non-invasive and reproducible manner by the selective and reproducible platform.
Source link: https://doi.org/10.1007/s10800-022-01673-2
This report looked at the regulatory implications of microRNA-1278 on airway inflammation, airway reconstruction, and the proliferation and apoptosis of airway smooth muscle cells caused by a transforming growth factor 1. MiR-1278 inhibition improved asthmatic mice's LTs, according to the study; miR-1278 expression was also upregulated in the blood and lung tissues of patients with asthma compared to that in healthy volunteers; miR-1278 expression was also upregulated in asthmatic mice; and miR-1278 inhibition raised the LTs of asthmatic mice.
Source link: https://doi.org/10.1007/s11010-022-04358-8
MicroRNA analysis is crucial in cancer diagnostics and therapy. MiRNA samples are typically labor intensive, and conventional methods used to extract miRNA for analysis are usually time-consuming. The entire extraction process was carried out in about 3 min, less than the commercial adsorption column method, which is less than the TRIzol method, which takes longer than 90 minutes, at more than 90 minutes. Good results for miRNA extraction in serum were obtained for miRNA extraction in serum, demonstrating the applicability of miRNA nucleic acid amplification by miRNA nucleic acid amplification.
Source link: https://doi.org/10.1007/s00216-022-03979-8
Exosomes are tiny bilayer-lipid membrane vesicles that are not visible by living cells that are able to move regulatory molecules and genetic information from one cell to another. Multiple human reproductive disorders, including polycystic ovarian syndrome, preeclampsia, uterine leiomyomata, ovarian cancer, endometriosis, and Asherman's syndrome have been documented in Aberrant microRNA expression in many human health disorders such as polycystic ovary syndrome, preeclampsia, uterine leiomyomata, endometriosis, Endometrio osia Exosomes have been shown to be a potential therapeutic treatment for several female reproductive disorders. In addition, changes in microRNA expression may act as molecular biomarkers for the diagnosis of several reproductive disorders in women, and restriction of their expression may increase infertility.
Source link: https://doi.org/10.1007/s43032-021-00556-9
Breast cancer is a multifactorial disease with multiple risk factors contributing to its pathogenesis. Several microRNAs have increased or decreased expression in metastatic breast cancer in recent years, serving as measures of metastatic activity in body fluids and tissue samples. The identification of these microRNA expression patterns may be helpful in the design of novel therapeutic molecules that mimic or inhibit microRNA activity.
Source link: https://doi.org/10.1007/s13346-021-00999-2
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