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Cell division cycle 20 and microRNAs are both highly expressed in non-small cell lung cancer. During NSCLC expansion, the new research sought to investigate the role of miR-1321 and miR-715 in CDC20. Using the TCGA database, the relationship between CDC20 expression and patient prognosis was explored. Using qRT PCR and western blotting, the expression profile of CDC20 in healthy lung cells and NSCLC cells was found. CDC20 was increased in NSCLC tissues and cells, thereby predicting the poor prognosis in NSCLC patients. The malignant phenotype of NSCLC cells was restored following CDC20 inhibition following CDC20 inhibition. MiR-1321 and miR-715 targeted CDC20 and 7515, which had the same anti-tumor effects as CDC20 silencing. The new research, therefore, has ramifications in NSCLC therapy and offers new insights into NSCLC management.
Source link: https://doi.org/10.1002/kjm2.12500
For miR-21 detection, identical tissue samples from 30 head and neck squamous cell carcinoma patients and eight head and neck cancer cell lines were obtained. MiR-21 expression among the 30 matched pair tissues was significantly elevated in 25 cancer tissues compared to the comparable control tissues; moreover, among the 8 HNC cell lines, miR-21 expression was significantly elevated in three, UPCI-4B, UMSCC-1, and UPCI-15B. The miR-21 mimic enhanced cell proliferation with reduced apoptosis and increased viability in both the UMSCC-1 and UPCI-4B cell lines, while the miR-21 inhibitor evoked the tumour suppressor phosphatase and tensin homologue, as well as inhibited PTEN expression; in addition, miR-21 antagonistically targeted the tumour suppressor phosphatase and tensin homologue and suspended PTEN expression; in both the UMSCC-1 In addition, the miR-21 mimic caused cisplatin resistance, while the miR-21 inhibitor restored cisplatin sensitivity. Targeting miR-21 can aid in cancer diagnosis, drug sensitivity, and therapeutic effects.
Source link: https://doi.org/10.1371/journal.pone.0267017
TGF-/Smad signaling plays a vital role in diabetic kidney disease's development. Here, we discovered that TGF- receptor 1, a key protein in TGF-/Smad signaling, was upregulated in kidney from diabetic mice and patients with DKD. In streptozotocin-induced DKD mice, prevention of miR-10a/b in the kidney by an in situ lentivirus-injection device attenuated collagen deposition and foot process effacement, whereas deprivation of miR-10a/b exacerbated renal fibrosis. Overexpressing miR-10a/b in kidney, according to our findings, may be a promising treatment for fibrosis in DKD.
Source link: https://doi.org/10.1016/j.omtn.2022.04.002
Exosomal miRNAs in porcine epidemic diarrhea virus infection were investigated in the present study, using exosomes isolated from Vero cells infected with PEDV. According to Nanoparticle tracking results, the average exosome particle size is 130. 5 nm. The results of miRNA sequencing revealed that a total of 115 miRNAs are abnormally expressed in the exosomes of infected cells, relative to the control group. Our results also provide critical information about the effects of PEDV infection on exosomal miRNA expression, as well as the search for potential anti-PEDV drug candidates.
Source link: https://doi.org/10.3390/v14040806
In diabetic patients with symptomatic internal carotid artery disease and underwent stent-supported angioplasty for ICAS, there is little known about the prognostic value of serum microRNAs in diabetic internal carotid artery disease. Following ICAS-PTA, the present study sought to determine expression levels of selected miRs for future major adverse cardiac and cerebral events as a predictor in diabetic patients. In 37 diabetic patients with symptomatic ICAS and 64 control group patients with symptomatic ICAS and 64 control group patients with symptomatic ICAS but not free of diabetes, the expression levels of 11 selected circulating serum miRs were compared to those in 37 diabetic patients with symptomatic ICAS and 64 control group patients with symptomatic ICAS but not free of diabetes. With symptomatic ICAS, diabetic versus non-diabetic patients with symptomatic ICAS may have different circulating miRs, according to our analysis. Higher expression levels of miR-134 in diabetic patients were prognostic for MACCE in diabetic patients, while elevated expression levels of miR-16 were prognostic in non-diabetic diabetic patients.
Source link: https://doi.org/10.3390/molecules27082472
Residents stem cells have a distinct metabolism, different from more committed progenitors and differentiated cells, making it tempting to conclude that resident stem cells have a distinct metabolism. Although screened for several stem cell species in vitro, in vivo data of niche-residing stem cell metabolism is sparse. We tested the hypothesis and examined the intestinal lineage from stem cell to differentiated epithelial cell in their native context under homeostatic conditions here, taking advantage of the genetically accessible adult Drosophila melanogaster intestine and the availability of ex vivo single cell sequencing results. The microRNA miR-277 is identified as a posttranscriptional regulator of fatty acid oxidation in the intestinal lineage, according to our first in silico analysis of single cell RNAseq results and functional experiments. In addition, research into FAO genes in silico has revealed that two groups of ISC exist in both mitotically active and quiescent ISC, of which the latter depends on FAO genes. In addition, we also looked at miR-277 effects on ISC in a benign and our newly developed CRISPR-Cas9-based cancer model, which in turn contributes to tumor formation, underlining the importance of FAO in a pathological context. Gaining more information about the metabolic variations and dependencies between two key and cancer-related cell populations in the fly and human intestine could lead to starting points for targeted combinatorial therapy in the hopes of improved treatment of colorectal cancer in the future.
Source link: https://doi.org/10.3390/metabo12040315
MicroRNAs have been shown to have a lot of promise as future diagnostic tools, with many responding to the need for additional biomarkers for prostate cancer diagnosis. We present a meta-analysis examining the diagnostic value and accuracy of circulating miR-375, one of the most commonly studied miRs in PCa. Methods and Methods: The diagnostic accuracy of miR-375 was tested using the QUADAS-2 unit, analyzing various statistical variables. 422 PCa patients and 212 controls were included in the seven studies that matched our selection.
Source link: https://doi.org/10.3390/medicina58040529
However, MSA and PSP patients' miRNA profiles are rare. The aim of this review was to critically assess miRNAs as diagnostic biomarkers in order to distinguish these atypical parkinsonian disorders and their role in disease pathogenesis. According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, a systematic literature search of PubMed was conducted up to February 2022. MiR-9-3p, miR-19b, and miR-24 are all potential MSA biomarkers, according to three research. MiR-132 was downregulated in two studies, but miR-147a and miR-518e were upregulated in PSP patients' brain tissue.
Source link: https://doi.org/10.3390/medicina58040483
Myeloid angiogenic cells, also known as early endothelial progenitor cells or circulating angiogenic cells, do not convert directly into developing blood vessels but rather raise angiogenesis in a paracrine manner. In HHT, MAC dysfunction has been identified. The role of miRNAs in HHT MAC dysfunction has yet to be documented to date. The aim of this study was to identify dysregulated miRNAs that may be responsible for the observed MAC dysfunction in HHT patients and control MACs. With a TaqMan miRNA microarray, twenty-three dysregulated miRNAs in HHT MACs were identified with a TaqMan miRNA microarray. MiR-132-3p is highly enriched in the TGF and AKT signalling pathways, targeting SMAD4, an effector of the TGF signalling pathway, and RASA1, a negative regulator of the PI3K/AKT signalling pathway, respectively, according to a bioinformatic review. These results show that miRNA expression modification can impair these pathways and contribute to MAC dysfunction in HHT.
Source link: https://doi.org/10.3390/genes13040665
Oral cancer is one of the top causes of death worldwide, with a 5-year survival rate of around 50% following treatment. MicroRNAs are small non-coding RNAs that play a key role in cancer formation by regulating signaling pathways involved in carcinogenesis. This paper aims to summarize the latest findings about oral cancer's evolving mechanisms due to risk factors and the role of miRNAs in these processes.
Source link: https://doi.org/10.3390/genes13040594
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