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MicroRNA - Crossref

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Last Updated: 23 April 2022

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MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway

Different expression patterns in the skin epithelium and its inflammatory overexpression in keratinocytes reduced proliferation, resulting in the production of fewer HFs with reduced hair bulb size and thinner hair growth. MiR-214 on HF development and cycling were linked to a variety of signaling pathways, including decreased expression of primary Wnt signaling mediators -catenin and Lef-1, and were rescued by therapy with pharmacological Wnt activators, but not inhibitors. -Catenin is one of the most conserved miR-214 targets in keratinocytes, and we conclude it with the inscription '-catenin. '.

Source link: https://doi.org/10.1083/jcb.201404001


A Soluble Epoxide Hydrolase Inhibitor Upregulated KCNJ12 and KCNIP2 by Downregulating MicroRNA-29 in a Mouse Model of Myocardial Infarction

Methods: Male 8-week-old C57BL/6 mice were divided into five groups and fed distilled water only or distilled water in varying dosages for seven days. KCNJ12, and KCNIP2 protein expression levels were determined by real-time PCR and KCNIP2 protein expression by western blotting, within 24 hours after MI to identify miR-29, KCNJ12, and KCNIP2 mRNA expression levels by western blotting. MiR-29 expression levels were significantly raised in the ischemic region of MI mouse hearts, as well as the mRNA and protein expression levels of its target genes KCNJ12 and KCNIP2 were significantly reduced.

Source link: https://doi.org/10.1532/hsf.2999


Downregulation of serum microRNA-205 as a potential diagnostic and prognostic biomarker for human glioma

OBJECT Circulating microRNAs are a new breed of highly promising cancer biomarkers. Here, the researchers analyzed serum miRNA 205 levels in patients with glioma. RESULTS The authors first reported that serum miR-205 expression in patients with glioma was significantly lower in patients with glioma than in healthy controls. Microbiometer levels of miR-205 were identified as an individual diagnostic marker and were significantly lower in the glioma cohort than in the other brain tumor cohorts. During glioblastoma recurrences, serum miR-205 levels were significantly higher in postoperative samples relative to those in the preoperative samples and were reduced again during glioblastoma recurrences. Low serum miR-205 expression, as well as both increasing pathological diagnoses and poor Karnofsky Performance Scale scores, were shown to have a significant correlation. Patients with glioma on an advanced pathological stage and a higher miR-205 serum level had longer overall survival than those with a lower miR-205 serum concentration.

Source link: https://doi.org/10.3171/2015.1.jns141577


MicroRNA-195 protection against focal cerebral ischemia by targeting CX3CR1

OBJECTIVE It has been reported that microRNA-195 helps prevent chronic brain injury caused by chronic brain hypoperfusion. METHODS The plasma levels of miR-195 expression in 96 patients with acute ischemic stroke were determined using real-time PCR, and the connection with the National Institutes of Health Stroke Scale score was evaluated. In mice exposed to middle cerebral artery occlusion with or without intra-cerebroventricular infusion of lentiviral vectors, cerebral infarct volume, clinical condition, and serums of miR-195 and CX3CR1 were tested. RESULTS In ischemic stroke patients, miR-195 was significantly reduced, and miR-195 in these patients' expression levels were negatively associated with the National Institutes of Health Stroke Scale score. MiR-195 is a novel target that controls neuroinflammation and brain injury, giving a novel treatment approach for cerebral ischemic disorders.

Source link: https://doi.org/10.3171/2018.5.jns173061


MicroRNA and gene expression changes in unruptured human cerebral aneurysms

The miRNA and mRNA interactions and expression levels in cerebral aneurysm tissue from human subjects were investigated. METHODS A prospective case-control research was conducted on human subjects to determine the differences in mRNA and miRNA expression in unruptured cerebral aneurysms in comparison to control tissue. MiRNA expression was measured using Affymetrix miRNA microarrays to measure miRNA expression, but NanoString nCounter technology was used to verify the identified targets. Several differentially expressed genes were discovered in aneurysm tissue, with MMP - 13 and various collagen genes being among the most restricted. MiR-21, miR-143, and miR-145 all had several significantly correlated target genes in the cohort that are associated with smooth muscle cell function, extracellular matrix remodeling, inflammation signaling, and lipid accumulation. CONCLUSIONS This review found differentially expressed genes and miRNAs in unruptured human cerebral aneurysms, raising the possibility of a role for miRNAs in aneurysm formation.

Source link: https://doi.org/10.3171/2015.11.jns151841


Stimulation of endothelial progenitor cells by microRNA-31a-5p to induce endothelialization in an aneurysm neck after coil embolization by modulating the Axin1-mediated β-catenin/vascular endothelial growth factor pathway

METHODS Adult male Sprague-Dawley rats were subjected to microsurgery to produce a coiled embolization aneurysm model and were administered with miR-31a-5p agomir or a negative control agomir via the tail vein for four weeks following IA induction. The effects of miR-31a-5p on EPC viability and operation of EPCs were also determined using Cell Counting Kit–8, wound-healing assay, and tube assays. Compared to a negative control agomir group, miR-31a-5p agomir improved endothelialization and increased the number of circulating EPCs in the peripheral blood. In addition, the number of vWF- and KDR-positive cells in the aneurysm neck in the miR-31a-5p agomir-treated group was increased. In addition, the miR-31a-5p's upregulation promoted EPC proliferation, migration, tube formation, and tube formation, as well as the expression of the proangiogenic factor vascular endothelial growth factor in vitro.

Source link: https://doi.org/10.3171/2019.5.jns182901


Identification of tumor suppressor gene LHPP-based 5-microRNA signature that predicts the early and mid-stage esophageal squamous cell carcinoma: a two-stage case-control study in the Chinese Han population

According to Abstract Global Cancer Statistics 2020, there were 544,076 deaths from esophageal cancer in 2020. In several solid tumor cells, phospholysine phosphate phosphatase is a key tumor suppressor gene, according to reports, but a similar expression in esophageal squamous cell carcinoma has yet to be identified. In addition, we investigated the expression profile of miRNAs involved in LHPP control by miRDB, TargetScan, and miRanda online prediction tools. Based on a research cohort of 75 ESCC patients and 75 healthy controls, a ESCC prediction model was developed. Finally, the predictive model was tested using a validation cohort of 45 ESCC patients and 45 healthy controls. LHPP protein levels in early and mid-stage ESCC tumor tissues were markedly reduced compared to adjacent tissues. In the early and mid-stage ESCC patients, the levels of the above five miRNAs and squamous cell carcinoma antigen in plasma were significantly elevated.

Source link: https://doi.org/10.21203/rs.3.rs-1562958/v1


Dynamic patterns of microRNA expression during acute myeloid leukemia state-transition

We are the first to show that the miRNA transcriptome undergoes state-transition during AML initiation and progression using serial samples from a mouse model of AML. To accurately predict time to AML in an independent cohort of mice, we modeled AML state-transition as a particle undergoing Brownian movement in a quasi-potential and state-transition model.

Source link: https://doi.org/10.1126/sciadv.abj1664

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions