Advanced searches left 3/3

Mice marrow cells - Crossref

Summarized by Plex Scholar
Last Updated: 17 April 2022

* If you want to update the article please login/register

The regulatory effect of miR-181c-5p on the differentiation function of bone marrow mesenchymal stem cells in postmenopausal osteoporotic mice

miR-181c-5p in BMMSCs were overexpressed or blocked by cell transfection, cell proliferation was reduced or inhibited by cell transplantation, and the developmental consequences of miR-181c-5p on BMMSC differentiation were investigated using MTT, multi-directional differentiation induction, alizarin red staining, qRT-PCR, and Western blot. Target gene prediction software and bioinformatics pages were used to analyze miR-181c-5p, and target gene prediction was performed, and target gene analysis was performed. Results The study found that overexpression of miR-181c-5p or banning miR-181c-5p had no effect on BMMSC proliferation. In addition, Foxo1 was reported as a direct target gene of miR-181c-5p, and miR-181c-5p negatively restricted Foxo1 expression. The overexpression of miR-181c-5p in the process of osteoporosis has resulted in the loss of osteogenic and adipogenic differentiation of BMMSCs in the process of osteoporosis, as well as a decrease in stem cell formation ability.

Source link: https://doi.org/10.21203/rs.3.rs-1453818/v1


Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta

After the infusion, there was a marginal but statistically significant rise in both collagen content and mineral content of bone 1 mo after the infusion. After 2. 5 weeks, donor male cells were transplanted into a female osteogenesis imperfecta-transgenic mouse, fluorescense in situ hybridization assays for the Y chromosome, including fibroblasts or fibroblast-like cells isolated in primary cultures of lung, calvaria, cartilage, long bone, tail, and skin, according to fluorescense in situ hybridization assays for the Y chromosome. In a parallel experiment in which whole marrow cells from a male mouse were transplanted into a female immunodeficient rag - 2 mouse, donor male cells constituting 4–6% of the fibroblasts or fibroblast-like cells in primary cultures. The findings support earlier findings that marrow stromal cells or closely related cells in marrow can act as a source of cell renewal in a number of nonhematopoietic tissues.

Source link: https://doi.org/10.1073/pnas.95.3.1142


Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice.

Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to track mesenchymal precursor cells from marrow cells that were partially enhanced by adherence to plastic, cultured, and then transplanted into irradiated mice. Following one week, no donor cells were present in the recipient mice, according to 1 week, but 1-5 months later, the donor cells accounted for 1. 5-12% of the cells in bone, cartilage, and lung in comparison to marrow and spleen.

Source link: https://doi.org/10.1073/pnas.92.11.4857


Persistence of dormant tumor cells in the bone marrow of tumor cell-vaccinated mice correlates with long-term immunological protection.

In syngeneic DBA/2 mice, live proliferation-competent and irradiated proliferation-incompetent L5178 murine lymphoma cells were compared for their ability to produce systemic anti-tumor immunity. The tumorigenic potential in live Eb cells was stifled by local knowledge of interleukin 4 or, alternatively, by injection of parental cells at a site that is resistant to tumor formation. Both CD4+ and CD8+ T lymphocytes are required for long-lived Eb or Eb-IL4 cells. Inoculation of nontumorigenic doses of live Eb or Eb-IL4 cells resulted in a long-lasting specific and systemic T-cell-mediated antitumor response that necessitated antitumorigenic T lymphocyte-mediated antitumor response. Following tumor cell inoculation, the more effective defense provided by vaccination with live tumor cells correlated with rapid migration and persistence of tumor cells in the bone marrow of host animals. Tumor cells were still expressed in the bone marrow of host animals injected with live Eb-IL4 cells, which were still expressed IL4. Dormant tumors in the bone marrow were correlated with the duration of anti-tumor immunity.

Source link: https://doi.org/10.1073/pnas.91.16.7430


Reconstitution of SCID mice with human lymphoid and myeloid cells after transplantation with human fetal bone marrow without the requirement for exogenous human cytokines.

Although engraftment of mouse bone marrow cells with human progenitor cells following infusion of human pediatric bone marrow cells was mainly dependent on continued therapy with exogenous human cytokines, the mouse bone marrow was not able to transplant human neural stem cells with human progenitor cells. Human precursor cells and monocytes were enriched with human marrow cells and their peripheral lymphoid compartment became populated with human B cells and monocytes in the present study, which followed pre-cultured human fetal bone marrow cells. In nine of nine BM-SCID-hu mice and macrophage-stimulating factor mRNA, as well as human cytokine gene expression, revealed human leukemia inhibitory factor mRNA and interleukin 7 mRNA in nine of nine BM-SCID-hu mice and macrophage-stimulating factor mRNA in seven of eight BM-SCID-hu mice.

Source link: https://doi.org/10.1073/pnas.91.17.8032


Recognition of beta 2-microglobulin-negative (beta 2m-) T-cell blasts by natural killer cells from normal but not from beta 2m- mice: nonresponsiveness controlled by beta 2m- bone marrow in chimeric mice.

The beta 2m gene mutation also affected the NK effector cell repertoire: beta 2m -/- mice failed to kill beta 2m -/- blasts, but they retained the ability to destroy the prototype NK cell target lymphoma YAC-1 at reduced rates. These results show that the absence of MHC class I/beta 2m protein is sufficient to make normal cells sensitive to NK cells, and that the same defect in a mouse's hemopoietic system makes its NK cells tolerant to beta 2m-deficient but otherwise normal cells. NK cells can be chosen or educated by other bone marrow cells in the beta 2m -/- mice to tolerate the MHC class I deficiency.

Source link: https://doi.org/10.1073/pnas.88.22.10332


Development of B-lineage cells in the bone marrow of scid/scid mice following the introduction of functionally rearranged immunoglobulin transgenes.

We asked whether the introduction of an IgM heavy-chain gene alone or both IgM heavy-chain genes in scid mice adds to early lymphoid cells committed to the B lineage, allowing further differentiation of scid pro-B cells into pre-B and B cells. We discovered that normal numbers of pre-B cells appeared in mu-transgenic scid mice's bone marrow, and that both pre-B and B cells were present in mu kappa-transgenic scid mice's bone marrow.

Source link: https://doi.org/10.1073/pnas.87.7.2730


Erythrocyte replacement precedes leukocyte replacement during repopulation of W/Wv mice with limiting dilutions of +/+ donor marrow cells.

The stem-cell-deficient W/Wv mouse's severe macrocytic anemia is eased by intravenous injection of normal marrow cells. The rate of replacement of the various hemopoietic cells in W/Wv transplant recipients was determined by exploiting host-donor differences in cellular markers. For the implantation of sufficient multipotent stem cells to alleviate the anemia, a dose of ten donor cells was required. Donythrocyte parameters were not significantly different from normal, and donor cells replaced at least 90% of the host erythrocytes within 20 weeks after injection.

Source link: https://doi.org/10.1073/pnas.85.19.7332


Enhancement of bone marrow allografts from nude mice into mismatched recipients by T cells void of graft-versus-host activity.

Engraftment of allogeneic T-cell-depleted bone marrow cells with or without mature thymocytes is T-cell dependent. A small inoculum of donor T cells must be supplemented with a trace number of donor T cells to ensure engraftment, whereas a small bone marrow dose from nude donors needs a much larger number of T cells for engraftment.

Source link: https://doi.org/10.1073/pnas.87.12.4595


Potential leukemic cells among bone marrow cells of young AKR/J mice.

Among bone marrow cells of AKR/J mice from the age of 14 days to the age of 14 days has been discovered potentially leukemic cells. The potential leukemic cells in young AKR mice's bone marrow cells were found to lack the presence of Thy-1. 1, in comparison to leukemic AKR cells that express Thy-1. 1. The transformation of preleukemic AKR marrow into overt leukemia in hybrid mice was largely dependent on the presence of an intact thymus and the recipients' x-ray exposure shortly before marrow transplantation.

Source link: https://doi.org/10.1073/pnas.77.5.2923

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

Search Recommendations

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions