Mevalonic Acid - Crossref

Mevalonic acid in human plasma: relationship of concentration and circadian rhythm to cholesterol synthesis rates in man.

After 4 weeks on a cholesterol-rich diet, we discovered that plasma MVA concentrations were correlated with elevated rates of whole-body cholesterol synthesis in patients treated with cholestryamine resin, which led to decreased rates of whole-body sterol synthesis in out-patients. In addition, our findings that the plasma MVA rhythm was suppressed by cholesterol feeding and abolished by a 12-day fast has hinted toward a relationship between the diurnal rhythm of plasma MVA and endogenous cholesterol synthesis.

Source link: https://doi.org/10.1073/pnas.79.9.3037

Neutrophils are required for the DNA synthetic response of human lymphocytes to mevalonic acid: evidence suggesting that a nonsterol product of mevalonate is involved.

When added to human lymphocytes, other organic acid anions, precursors of mevalonic acid biosynthesis and a variety of mevalonate metabolism inhibitors, are unable to initiate DNA synthesis. When the cells are cultured in lipoprotein-containing medium, the reaction to mevalonic acid of ML-236B-inhibited lymphocytes is elevated, and the threshold value of meyalonate, which is where lymphocyte DNA synthesis first appears, is reduced. The reaction of lymphocyte cultures cultured in lipoprotein-depleted medium can be enhanced by addition to low density lipoprotein, but not by the addition of high density lipoprotein.

Source link: https://doi.org/10.1073/pnas.79.9.3028

Measurement of daily cholesterol synthesis rates in man by assay of the fractional conversion of mevalonic acid to cholesterol.

A significant correlation has been found in man between total daily cholesterol synthesis rates as determined by sterol balance tests and the percentage of intravenously administered R-[5-14C]mevalonic acid converted to cholesterol. According to 30 studies, the mean daily cholesterol synthesis rates estimated by sterol balance experiments varied from 395 to 3047 mg/day, whereas the fractional conversion of mevalonate to cholesterol varied from 0. 28 to 0. 99.

Source link: https://doi.org/10.1073/pnas.74.7.3043

A Novel NAD + /MWNTs Nanocomposite-Based Electrochemical Biosensor for Measuring Mevalonic Acid

The current is linear, with MA ranging from 18. 1 to 145 M with a limit of detection down to 4. 2 M, and the sensor displaying a sensitivity of 92. 2 mm/mM at the NAD + MWNTs-modified SPE.

Source link: https://doi.org/10.4028/www.scientific.net/kem.605.388

Synthesis of Heterologous Mevalonic Acid Pathway Enzymes in Clostridium ljungdahlii for the Conversion of Fructose and of Syngas to Mevalonate and Isoprene

ABSTRACT BACTING: There is a growing interest in the use of microbial fermentation for the production of high-demand, high-purity chemicals using inexpensive feedstocks in an environmentally friendly manner. Isoprene, a hemiter pene that is mostly polymerized to polyisoprene in synthetic rubber in tires, is one of the many examples investigated here is the manufacturing of isoprene, a hemiterpene that can be converted to C 10 and C 15 biofuels. These plasmids, one or two of which were introduced into the host cells, enabled the synthesis of mevalonate and isoprene from fructose, as well as the conversion of mevalonate to isoprene. All of the MVA's heterologous enzymes, as well as Idi and IspS, were shown to be synthesized at high levels in C. ljungdahli's physiology, and were enzymatically active, as shown by in vivo product synthesis. The quantities of mevalonate and isoprene produced here are much less than those that would be needed of a commercial production strain. This report reveals the ability to synthesize a heterologous metabolic pathway in C. ljungdahlii, an organism that can digest either simple sugars or syngas, or both simultaneously. The ability of C. ljungdahli to grow mixotrophically also allows the recapture, if there are no suitable reducing alternatives to the CO 2 released on glycolysis, potentially increasing the mass yield of product formation.

Source link: https://doi.org/10.1128/aem.01723-17

Production of Mevalonic Acid by Fermentation

The aim of this research was to find microorganisms that produce substantial amounts of mevalonic acid and to produce an economic fermentation process. Assay design is based on the principle of the assay based on the organism Lactobacillus heterohiochii's crucial mevalonic acid requirement for growth. After changes of medium and fermentation conditions, Endomycopsis fibuliger was chosen for medium growth studies, and 939 g of mevalonic acid per ml was produced in the culture filtrate.

Source link: https://doi.org/10.1128/am.16.7.965-972.1968

Clinical and Biochemical Phenotype in 11 Patients With Mevalonic Aciduria

Mevalonic aciduria is a result of the deficiency of mevalonate kinase, the first enzyme after 3-hydroxy-3-methyl hydroxy esterified-coenzyme A reductase in the biosynthesis of cholesterol and nonsterol isoprenes. Mevalonic acid in body fluids was determined by stable isotope chromatography/mass spectroscopy with targeted ion monitoring, ubiquinone-10 concentrations by reversed-phase high-pressure liquid chromatography. Patients with Less' most severe conditions have suffered with psychomotor retardation, hypotonia, myopathy, and ataxia. Mevalonic acid levels were found to be grossly elevated in body fluids of both patients. Concentrations of plasma cholesterol were either normal or marginally reduced. In the majority of patients, ubiquinone-10 concentrations in plasma were found to have been reduced. Although patients with mevalonic aciduria have a recognizable pattern of severe medical signs, some patients are likely to remain undiagnosed, which can be found in a variety of subspeciality clinics, including neurology, troenterology, cardiology, and genetics.

Source link: https://doi.org/10.1542/peds.91.5.915

Case Report: Mevalonic Aciduria Complicated by Acute Myeloid Leukemia After Hematopoietic Stem Cell Transplantation

Mevalonic aciduria is the most common clinical subtype of mevalonate kinase deficiency caused by a genetic defect in the mevalonate pathway. MKD patients' autoinflammatory disease problems were shown to be able to be alleviated by allogeneic hematopoietic stem cell transplantation. After transplantation, the patient died of sepsis on day 4 after transplantation. A second HCT from her haploidentical father was performed to diagnose acute myeloid leukemia. Our results show that normalizing the mevalonate pathway after HCT in patients with MKD differs from patient to patient, depending on the clinical spectrum and severity of disease.

Source link: https://doi.org/10.3389/fimmu.2021.782780

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