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Methylation Genes - Crossref

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Last Updated: 15 May 2022

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Development of a Risk Score Model for Osteosarcoma Based on DNA Methylation-Driven Differentially Expressed Genes

Osteosarcoma is the most common malignant bone tumor in adolescent patients, and patients are vulnerable to transplantation, tumor metastasis, chemotherapy resistance, and even death. We investigated the potential relationship between abnormal methylation differentially expressed genes and osteosarcoma patient survival rates. To determine osteosarcoma risk genes, the RNA-seq and clinical results from TCGA's TARGET-OS project were used for univariate and least absolute shrinkage as well as selection operator Cox regression analyses to determine osteosarcoma's risk genes. The risk score model was based on 50 hypermethylation-downregulated genes and 4 hypomethylation-upregulated genes, with 50 hypermethylation-downregulated genes and four hypomethylation-upregulated genes identified later, based on LASSO Cox regression analysis for 54 aberrant methylation-driven genes and 4 hypomethylation-upregulated genes. The three genes that were regulated by DNA methylation were found to correlate with OS patients' outcomes, which may provide new insight into osteosarcoma's pathological mechanism.

Source link: https://doi.org/10.1155/2022/7596122


Age-Related DNA Methylation in Normal Kidney Tissue Identifies Epigenetic Cancer Risk Susceptibility Loci in the ANKRD34B and ZIC1 Genes

The TCGA-KIRC's results were biometrically analyzed to find new loci with age-dependent DNA methylation that may be contributing to tumor risk in normal kidney tissue. A strong relationship was found between relative methylation levels and age, and seven out of ten loci were able to predict chronological age in normal kidney tissues, accounting for 84% of the variance. Our results show the presence of additional methylation risk factors besides age for renal cancer in normal kidney tissue.

Source link: https://doi.org/10.3390/ijms23105327


Flavonoid Biosynthesis Genes in Triticum aestivum L.: Methylation Patterns in Cis-Regulatory Regions of the Duplicated CHI and F3H Genes

Flavonoids are a diverse group of secondary plant metabolites that play a vital role in plant formation and protection against stressors. In the allohexaploid bread wheat Triticum aestivum L. , a functional divergence between some of the structural TaCHI and TaF3H genes was first demonstrated. The predicted position of CpG islands in the analyzed genes' promoter regions of the analyzed genes was found to differ, as well as the actual location of methylation sites did not match. For the first time, we discovered that methylation status could influence the expression of TaCHI copies, but not so much as TaF3Hs. We believe that the TaF3Hs' promoter system plays mainly on gene expression's change.

Source link: https://doi.org/10.3390/biom12050689


Methylation of p53-responsive oncosuppressive microRNA genes in hemoblastosis

The aim of the investigation was to provide up-to-date data on the presence and effects of a slew of p53-responsive oncosuppressive micrornas genes methylation in malignant neoplasms of the blood system's malignant neoplasms. The p53 protein controls a large number of microRNAs, which are highly conserved small RNA molecules that influence gene expression mainly by blocking translation. In this regard, it should be noted that positive feedback about p53 and microRNAs triggered by it, as well as negative feedbacks regarding p53-responsive micrornas and C-MYC and DNA methyltransferases should be included. The findings in the study, on the other hand, clarify the current understanding of the role of the p53 protein and the micrornas triggered by it, as well as the functional association of p53-responsive microRNAs.

Source link: https://doi.org/10.21294/1814-4861-2022-21-2-130-142


DNA methylation analysis of tumor suppressor genes in liquid biopsy components of early stage NSCLC: a promising tool for early detection

Abstract Purpose Circulating tumor cells and circulating tumor DNA analysis are two new ways to track tumor evolution in real-time. Early stage NSCLC is a form of cancer suppressor genes found in CTC and matched ctDNA, providing prognostic information in a young stage NSCLC. In: a trial group of 35 primary tumors and their corresponding non-cancerous tissues of early stage NSCLC patients, a validation group of 22 primary tumor tissues and 42 peripheral blood samples of early stage NSCLC patients, a research group of five selected gene promoters was investigated by highly precise and sensitive real-time methylation specific PCR assays. b at least one identified methylated gene promoter in plasma-cfDNA or plasma-cfDNA P = 0. 015 was isolated from FFPEs, CTCs promoter methylation of APC and SLFN11 in plasma-cfDNA, and b at least one identified methylated gene promoter in CTC or plasma-cfDNA P = 0. 015.

Source link: https://doi.org/10.1186/s13148-022-01283-x


m7G Methylation-Related Genes as Biomarkers for Predicting Overall Survival Outcomes for Hepatocellular Carcinoma

Aim: The quest for prognostic biomarkers and the design of a prognostic risk model for hepatocellular carcinoma based on N7-methylation regulators lead to a hepatocellular carcinoma. According to the median risk score, each patient's risk score was calculated using the calculator, and patients were divided into high- and low risk groups. The Kaplan-Meier survival study revealed poorer 5-years overall longevity in the high-risk group compared to the low-risk group, as well as a low-risk group, and the receiver-operating characteristic curve suggested improved model prediction. The Cox regression investigation included model risk scores and clinicopathological information, and the findings showed that a high risk score was the sole independent risk factor for patients with HCC's prognosis. Conclusions: The validated bioinformatics-based prognostic risk model for HCC was found to have good predictive power.

Source link: https://doi.org/10.3389/fbioe.2022.849756


Identification of Differential Genes of DNA Methylation Associated With Alzheimer’s Disease Based on Integrated Bioinformatics and Its Diagnostic Significance

Introduction Alzheimer's disease is a common neurodegenerative disorder. DNA methylation has been closely linked to AD pathogenesis, according to recent research, which has shed light on potential biomarkers for early AD diagnosis and related potential therapeutic approaches. Methods The GEO database's two databases were used to obtain Alzheimer's disease samples and healthy controls data. To find differentially expressed genes, use the LIMMA software package in R language. The top ten Hub genes were discovered on the basis of the PPI network using the CytoHubba plugin, and the AUC areas of these top-ranked genes were all greater than 0. 7, demonstrating acceptable diagnostic accuracy. Conclusion The study found the top ten Hub genes related to AD-related DNA methylation, of which RPSA, RPS23, and RPLP0 have high diagnostic accuracy and excellent AD biomarker potential.

Source link: https://doi.org/10.3389/fnagi.2022.884367


PGC-1α Methylation, miR-23a, and miR-30e Expression as Biomarkers for Exercise- and Diet-Induced Mitochondrial Biogenesis in Capillary Blood from Healthy Individuals: A Single-Arm Intervention

Exercise has been suggested as the best way to maintain healthy mitochondria during aging; strength training has also been shown as vital to health and healthy aging. We therefore investigated the effects of concurrent exercise training and dietary habits on epigenetic pathways involved in mitochondrial function and biogenesis. We analyzed epigenetic biomarkers that specifically target mitochondrial biogenesis, PGC-1, and mtDNA samples. Before and after the intervention, dried blood spot samples and reports on diet, lifestyle, and body composition were collected. MiR-23a, miR-30e expression, and mtDNA samples were analyzed using real-time quantitative polymerase chain reaction analysis. Following the treatment, MiR-23a, miR-30e expression, and PGC-1 methylation decreased after the intervention. Our findings show that concurrent training could enhance mitochondrial biogenesis and functions by changing the epigenetic code.

Source link: https://doi.org/10.3390/sports10050073


DNA methylation reprogramming during oogenesis and interference by reproductive technologies: Studies in mouse and bovine models

Since the birth of the first baby conceived by ART over 30 years ago, the use of assisted reproductive technologies to solve fertility problems has continued to rise. DNA methylation is the most widely researched of these; for healthy reproduction, the optimal establishment of DNA methylation patterns in gametes and early embryos is crucial for healthy growth. According to data available from mouse and bovine models, the present review explores the effect of ART on oocyte and embryo DNA methylation with respect to oocyte and embryo DNA methylation.

Source link: https://doi.org/10.1071/rd14333


DNA Methylation Patterns of Glucocorticoid Pathway Genes in Preterm Birth Among Black Women

These statistics are particularly troubling for non-Hispanic Black women in the United States who give birth prematurely at 1. 5 times higher rates than non-Hispanic White women. According to previous studies, psychological stress is associated with PTB in Black women. In six glucocorticoid, stress-related genes in 44 pregnant Black women, we investigated DNA methylation in peripheral blood leukocytes. The ability to identify stress-related biological markers that are associated with PTB among Black women will be a major step toward eliminating PTB disparities among these women.

Source link: https://doi.org/10.1177/10998004221099253

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions