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Methylation - Crossref

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Last Updated: 23 May 2022

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DNA methylation of

At the mononuclear pollen stage, in wheat, S-type cytoplasmic male sterile lines can generally transition from sterility to fertility. We measured amounts of key carbohydrates and enzymes of sucrose metabolism at the mononuclear pollen stage in anthers collected from an S-CMS line and its maintainer line, and found that nonreducing sugars increased in S-CMS anthers. During starch accumulation in pollen, the presence of invertase has declined sharply, though sucrose synthase operation during starch accumulation in pollen showed no change in S-CMS anthers at the mononuclear pollen stage. The determination of ethylene anabolism was determined because there is a strong correlation between the rate of ethylene evolution and sucrose content. Lower methylation was detected in the S-CMS line, and we investigated the DNA methylation pattern of TaACS2 in the core promoter region using bisulfite genomic sequencing.

Source link: https://doi.org/10.1071/cp21630


De novo DNA methylation through 5'-segment of the H19 ICR maintains its imprint during early embryogenesis

Genomic imprinting is a key monoallelic gene expression regulator in mammals that is dependent on gamete-specific DNA methylation of specialized cis-regulatory elements known as imprinting control regions. Here we show that methylation of the paternally inherited transgenic H19 ICR begins shortly after fertilization in a maternal Dnmt3a- and Dnmt3L-dependent manner. We finally figured out the causal sequences for this disease in transgenic mice, finding that deletion of the 5' segment of the endogenous paternal H19 ICR reduced its methylation after fertilization, which attenuated Igf2 gene expression. These results show that this segment of the H19 ICR is essential for its de novo post-fertilization DNA methylation and that this activity aids in the maintenance of imprinted methylation at the endogenous H19 ICR during early embryogenesis.

Source link: https://doi.org/10.1242/dev.126003


Lsh Is Essential for Maintaining Global DNA Methylation Levels in Amphibia and Fish and Interacts Directly with Dnmt1

DNA methylation is missing in Lsh depleted frog and fish embryos, both of which have developmental delay, and here we show that DNA methylation is lacking in Lsh depleted frog and fish embryos. In addition, we find that chromatin and Dnmt1 are related to chromatin, and that Lsh and Dnmt1 are linked with chromatin, and that a reduced Dnmt1-chromatin relationship can be attributed to chromatin declines. These findings show that the role of Lsh in DNA methylation is conserved in plants, amphibian, fish, and mice, as well as support a model in which Lsh's contribution to Dnmt1 binding to chromatin can lead to disruptions in DNA methylation maintenance.

Source link: https://doi.org/10.1155/2015/740637


Methylation of the Corticotropin Releasing Hormone Gene Promoter in BeWo Cells: Relationship to Gene Activity

Here we determined whether methylation of the CRH proximal promoter regulates basal and cAMP-stimulated CRH expression in BeWo cells, a well-characterized trophoblastic cell line. We treated the cells with 8-Br-cAMP and the DNA methyltransferase inhibitor 5-aza-2′ deoxycytidine, determined the effects on CRH mRNA level and promoter methylation, as well as promoter methylation. CRH mRNA expression and the methylation of a subset of CpGs increased spontaneously during culture, as a result of culture. CRH gene expression was not affected by the conditions used, but rather it established the contribution of alternative cAMP-independence pathways and cAMP-independent pathways to CRH expression control, which was not limited by methylation.

Source link: https://doi.org/10.1155/2015/861302


Bioinformatics Analysis of the Characteristics and Correlation of m6A Methylation in Breast Cancer Progression

According to the analysis of breast cancer gene expression characteristics, the survival rate of breast cancer samples of the four subtypes also differs, and surprising differences in the number of exons skip among the four subtypes among the four subtypes can be seen. A prominent rise in the copy number of RAD54B of the protein-binding subtype is seen in the DNA damage repair genes, but there are few mutations in other DNA damage repair genes and copy number deletion is everywhere. In addition, reports of the highest tumor stemness index and the lowest in breast cancer samples of m6A methylated type can highlight the critical role of the high expression of m6A reader protein in breast cancer progression.

Source link: https://doi.org/10.1155/2022/4416439


Association of DNA Methylation of the NLRP3 Gene with Changes in Cortical Thickness in Major Depressive Disorder

The Nod-like receptor pyrin containing three inflammasome has been reported to be a convergent point relating the peripheral immune response induced by psychological stress and neuroinflammatory processes in the brain. We wanted to find similarities in the methylation profiles of the NLRP3 gene between major depressive disorder patients and healthy controls. Using magnetic resonance imaging results, we also investigated the connection of the methylation score of loci in NLRP3 with cortical thickness in the MDD group. Our results show that increasing NLRP3 DNA methylation in MDD may lead to depression-related brain structural changes.

Source link: https://doi.org/10.3390/ijms23105768


Heterosis of growth trait regulated by DNA methylation and miRNA in allotriploid fish

Abstract Background Heterosis of growth traits in allotriploid fish has aided in aquaculture's production for many years, but the genetic and molecular basis of the species has remained vague. In combination with gene expression in the allotriploid complex, we performed a series of tests on DNA methylation modification and miRNA expression. DNA methylation was found to contribute to the emergence of a dose-limiting condition, which reduced gene expression in the triploid to the diploid state, according to Then, comparative analyses. Lastly, the coevolution of small RNAs and their homoeologous targets were documented and used to determine miRNA expression in the allotriploids. Conclusions Our findings revealed the link between DNA methylation and miRNA in allotriploids, which not only helps us learn about the genetic basis of heterosis of growth traits but also supports the study and application of epigenetics in aquaculture.

Source link: https://doi.org/10.1186/s13072-022-00455-6


H3K27 methylation regulates the fate of two cell lineages in male gametophytes

Abstract Microspores divide to produce a vegetative cell and a male germline during angiosperm male gametogenesis, each with distinct cell fates. Here, we find that H3K27me3 is essential for VC fate for male Arabidopsis thaliana gametophytes; the H3K27me3 erasure aids in cell fate generation in male Arabidopsis thaliana gametophytes. The H3K27me3 erasure, according to H3K27me3 erasure, stunted VC growth and moved the VC to a gamete destination, meaning that MG cells require H3K27me3 erasure to trigger gamete cell fate.

Source link: https://doi.org/10.1093/plcell/koac136


Prediction of methylguanine methyltransferase promoter methylation in glioblastoma using dynamic contrast-enhanced magnetic resonance and diffusion tensor imaging

Object The methylation status of the methyltransferase promoter has been correlated with therapy response in glioblastoma. The study included 43 patients with pathologically diagnosed glioblastoma, who had undergone preoperative DCE-MRI and DTI, and whose MGMT methylation status was known. To find the correct cutoff value for the presence of MGMT methylation, the authors conducted receiver operating characteristic curve simulation. Results MGMT methylation on conventional MR images was not significantly related to any imaging features. Ktrans values in the MGMT methylated group were noticeably higher. Ktrans > 0. 086 min 1 was the most accurate cutoff value for MGMT methylation at an area under the curve of 0. 6 percent, with a sensitivity of 56. 3 percent and a specificity of 85. 2%. Conclusions Ktrans can be used as a potential imaging biomarker to predict MGMT methylation status preoperatively in glioblastoma, but further research into a larger population is required.

Source link: https://doi.org/10.3171/2014.5.jns132279


MGMT promoter methylation in patients with glioblastoma: is methylation-sensitive high-resolution melting superior to methylation-sensitive polymerase chain reaction assay?

Although pyrosequencing is the gold standard for determining the methylation status of MGMT, methylation-sensitive polymerase chain reaction assay continues to be used widely. METHODS The authors produced three primer sets ranging from CpG 72-89 for MS-HRM analysis to determine the methylation levels of 6 human glioma cell lines. The authors also submitted surgical samples from 75 GBM patients treated with temozolomide to MS-HRM to determine the MGMT methylation status and compared the results to MS-PCR results using receiver operating characteristic, univariate, and multivariate analysis. Conclusions and conclusions There was a strong correlation between the methylation levels of the six glioma cell lines tested by MS-HRM and by bisulfite sequencing; with primers 1 and 2, the correlation was evident. Patients with GBM have been recommending MS-HRM as an alternative to MS-PCR for determining the prognosis of patients with GBM.

Source link: https://doi.org/10.3171/2017.11.jns171710

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions