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Methyl - BioProject

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Last Updated: 20 June 2022

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CDDO drives transcriptomic and epigenetic reprogramming in response to TPA-induced JB6 cell neoplastic transformation [Methyl-seq]

"CDDO is a potential antioxidative and antiinflammatory agent that can reduce the TPA-induced skin cell transformation at a nanomolar scale. " Overall scheme: Samples of JB6 cells were used for DNA and RNA extraction; DNA was enhanced by Agilent SureSelect Kit; then bisulfite converted methyl-seq was carried out;.

Source link: https://www.ncbi.nlm.nih.gov/bioproject/849520


9-methyl sphingoid base generated by cleavage of pathogen-derived ceramide is recognized by a lectin receptor-like kinase in Arabidopsis thaliana

"We discovered two new genes necessary for immunity in Arabidopsis thaliana using Lumi-Map. " In Arabidopsis, we demonstrate that a ceramide PiCerD derived from an oomycete pathogen Phythora infestans causes a defense response. A PiCR of Arabidopsis is cleaved by Arabidopsis apoplastic ceramidase PiCD, and the resulting 9-methyl branched sphingoid base is identified by a plasmamembrane kinase PiCR of Arabidopsis. PiCR and PiCD's "functions were harmed by PiCR's failure against the oomycete pathogen, indicating their vital roles in the defense. ".

Source link: https://www.ncbi.nlm.nih.gov/bioproject/841866


Transcriptomic analysis of fumarate compounds identifies unique effects of isosorbide di-(methyl fumarate) on NRF2, NF-kappaB and IRF1 pathway genes

"We compared the effects of fumarates on gene expression in astrocytes. " IDMF responses were contagious, with both NRF2 activation and NF-block inhibition, as well as IDMF blocking, as we reported IDMF-mediated NF-kappaB blockage using a reporter assay. IDMF also down-regulated IRF1 expression and IDMF-decreased gene promoters were given IRF1 recognition sequences, and IDMF-enriched gene promoters were enriched with IRF1 recognition se-quences. The most significant effect on MS-associated genes was found by each fumarate relative to those near loci from MS genetic association studies, but IDMF had the largest overall effect on MS-associated genes. These results indicate that next-generation fumarates such as DRF and IDMF have differing effects from those of the MMF metabolite. Our results support a model in which IDMF attenuates oxidative stress via NRF2 activation, with NF-kappaB and IRF1 suppression contributing to the prevention of inflammation and pyroptosis. "We treated human fetal astrocytes with sterile distilled water, MMF, DRF, or IDMF for 24 hours and determined gene expression using Clariom S microarray assays. ".

Source link: https://www.ncbi.nlm.nih.gov/bioproject/825247


Genetic effects on non-CG DNA methylation [Methyl-C-seq]

"The epigenome, in particular, variation of DNA methylation profiles among individuals, has long been of concern as a code modifier," with u201cmutations reflecting past environments, stochastic activities, or genetic regulation. ".

Source link: https://www.ncbi.nlm.nih.gov/bioproject/800441


methyl-Seq of Pediatric ARDS Nasal and Bronchial Brushings

"The aim of this research was to find differences in the nasal methylome of patients with Pediatric ARDS compared to controls, as well as testing how this would change over time. " Subgroup 1 was made up entirely of PARDS subjects, and Subgroup 2 contained both control and PARDS subjects. Over time, the methylation pattern of PARDS subjects changed, but the pattern of control subjects remained stable. ".

Source link: https://www.ncbi.nlm.nih.gov/bioproject/793833


Integrative analysis of gene expression and DNA methylation reveals epigenetic regulation of tumor suppressor genes and oncogenes involved in uterine leiomyoma pathogenesis [Methyl-Seq]

"Uterine leiomyomas are the most common benign tumors in women of reproductive age. " In particular, DNA methylation plays a significant role in gene expression regulation. We want to determine the relationship between DNA methylation and gene expression in UL in comparison to adjacent myometrium in order to identify molecular mechanisms involved in UL formation that are under epigenetic control. Our results revealed a different DNA methylation profile between UL and MM, resulting in hypermethylation of UL, as well as a new global transcriptome profile. 93 genes were regulated by methylation, 22 hypomethylated/upregulated, and 71 hypermethylated/downregulated were all regulated by integration of DNA methylation and transcriptome information. In conclusion, DNA methylation gene regulation is implicated in UL pathogenesis, and its reversion may be a promising therapeutic treatment. Overall concept: The prospective study of DNA methylation by methyl-seq and gene expression by RNAseq of uterine leiomyoma versus adjacent myometrium tissue "" according to a generalist.

Source link: https://www.ncbi.nlm.nih.gov/bioproject/791235

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions