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Methotrexate Toxicity - Crossref

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Last Updated: 02 May 2022

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P068 Monitoring of methotrexate blood toxicity during the COVID-19 pandemic for patients with rheumatoid arthritis: data from the Greater Manchester Care Record

Abstract Background/Aims The current British Society for Rheumatology's proposal for patients on methotrexate with 3-month blood testing is recommended, but research supporting this treatment is limited. The study sought to establish: 1 interval length between blood tests during the pandemic and 2, and 2 whether prolonged intervals were related to abnormal blood test results. Methods Data came from the Greater Manchester Care Record, a website that holds electronic health records from both primary and secondary care for residents throughout Greater Manchester. In the year leading up to the pandemic 01/03/2019-01/03/2020, inclusion criteria included: a diagnosis of RA; regular blood monitoring =91 days between blood tests and regular methotrexate prescriptions. During the pandemic and a median interval of 57 days IQR: 34-84, 1011 patients met the inclusion criteria, with a median of 5 blood tests interquartile range IQR: 3-7. Some 115 11. 4% people had a long wait but no subsequent blood test was done before the study's end date, median 120 days [IQR: 104-150]. According to time-periods and interval lengths, the percentage of cytopenia and transaminitis events was low and constant throughout time-periods and when stratified by interval length Table 1. Conclusion Nearly two-thirds of patients on stable methotrexate had at least two weeks in interval, and 1 in ten had a long time with no subsequent blood test. Initial analyses of test results indicate that methotrexate blood toxicity in those with longer blood test intervals during the pandemic do not rise.

Source link: https://doi.org/10.1093/rheumatology/keac133.067


The influence of different concentrations of aqueous green tea extract on methotrexate induced haematological toxicity in rats

The group, which was in thism group rats, was treated with a 1. 2 percent concentration of AGTE for 12 days, and the individual in thism group rats received different amounts of AGTE as their sole source of drinking water, 7days before and 5 days after MTX therapy. In addition, the concentrations of AGTE were found at a concentration of 1. 85 percent, while 0. 62 percent and 2. 55 percent respectively had no protective effects on haematological parameters.

Source link: https://doi.org/10.32947/ajps.v13i1.185


Is hypoalbuminemia a risk factor for high-dose methotrexate toxicity in children with acute lymphoblastic leukemia?

Abstract Background The repeated use of high-dose methotrexate is a common component of modern childhood acute lymphoblastic leukemia treatment regimens. The purpose of this research was to investigate the correlation between pre-infusion serum albumin and potential HDMTX toxicities. Plasma MTX levels were estimated at 24 h from the start of HDMTX infusion in the first consolidation cycle, about 24 h from the start of the infusion cycle. Before HDMTX administration, serum albumin level was determined, but pre-infusion hypoalbuminemia was defined when serum albumin was below 3. 5 g/dL. Oral mucositis was more prevalent in the 2. 5 g/m 2 than the 5 g/m 2 HDMTX cycles. Conclusions Serum albumin levels should be checked before starting HDMTX cycles, especially in resource-limited settings where malnutrition is common, and serum MTX analysis may not be available. Optimizing serum albumin levels before HDMTX can help reduce the possibility of HDMTX-toxicities.

Source link: https://doi.org/10.1186/s43046-022-00122-7


Epicatechin ameliorative effects on methotrexate-induced hepatotoxicity in mice

Background Due to the fact that methotrexate is both an immunosuppressive drug and as a chemotherapy agent, several research is needed to minimize the side effects of this drug on non-target organs. Purpose This study was designed to investigate the effects of epicatechin on mouse hepatotoxicity MTX-induced hepatotoxicity. Research Design We randomly divided 42 male Naval Medical Research Institute mice into six groups for adaptation: Epi; MTX on the fifth day; and Epi + MTX on the fifth day, with Epi + MTX on the fifth day. In addition, MTX reduced glutathione level and activity level of catalase, superoxide dismutase, and glutathione permeate. Epi was able to reduce the toxic effects of oxidative/antioxidant system imbalance, inflammation, and even histopathological destruction in MTX-intoxicated mice. Epi goes into use as a therapeutic agent in hepatotoxicity linked to MTX chemotherapy, according to the authors.

Source link: https://doi.org/10.1177/09603271211047924


Accumulation of DNA strand breaks and methotrexate cytotoxicity.

Cells that were treated with methotrexate were characterized as a pattern of strand breaks in Ehrlich's mature DNA. During the first ten hr of incubation with 2 microM methotrexate, DNA single-strand breaks accumulated in a linear fashion as a result of age. The amount of DNA fragment breakage in cells that had been incubated with methotrexate for 24 hours was as high as in cells that had been irradiated with 300 rads. After exposure to methotrexate, hypoxanthine, and nonessential amino acids, cell fractures persisted in cells that were incubated, indicating that these strand breaks were poorly repaired. Cell death was attributed to a lethal accumulation of DNA strand breaks, according to these results. The occurrence of DNA strand breaks is most likely due to poor DNA repair that has resulted from the inhibition of syntheses of thymidylate and purine nucleotides.

Source link: https://doi.org/10.1073/pnas.81.18.5694


Antibody-targeted liposomes: increase in specific toxicity of methotrexate-gamma-aspartate.

When encapsulated in targeted liposomes, the ability of methotrexate-gamma-aspartate to inhibit L929 growth is enhanced 10-fold, but it is reduced to 50% when encapsulated in liposomes with no specificity for the target cells. Soluble antibody does not inhibit the absorption of targeted vesicles, but empty targeted vesicles do reduce the effectiveness.

Source link: https://doi.org/10.1073/pnas.80.5.1377


Cytotoxicity of 5-fluoro-2'-deoxyuridine: requirement for reduced folate cofactors and antagonism by methotrexate.

5-fluoro-2'-deoxyuridine's cytoxicity in cultured L1210 mouse leukemia cells is reduced when intracellular decreased folates are depleted, either by limiting the source in media or blocking dihydrofolate reductase with methotrexate. Likewise, the intracellular amount of 5-fluoro-2'-deoxyuridylate covalently bound to thymidylate synthase in L1210 cells treated with 5-fluoro-2'-deoxyuridine is greatly reduced when cells are depleted of folate cofactors.

Source link: https://doi.org/10.1073/pnas.75.2.980


Role of thymidylate synthetase activity in development of methotrexate cytotoxicity.

Both thymidine or hypoxanthine inhibited mouse or human leukemia cells in culture, with Methotrexate inhibition of mouse or human leukemia cells. In addition, citrovorum factor-induced reversal of MTX was several orders of magnitude more effective in the presence of both FdUrd and dThd than either in the presence of dThd alone or in the absence of both nucleosides. In addition, the presence of FdUrd and dThd prevented the lethality of 0. 3 mM MTX to L1210 cells in culture medium supplemented with micromolar amounts of citrovorum factor.

Source link: https://doi.org/10.1073/pnas.76.11.5924


MTHFR Gene Polymorphism Association With Psoriatic Arthritis Risk and the Efficacy and Hepatotoxicity of Methotrexate in Psoriasis

Aims To determine whether MTHFR rs1801131 and rs1801133 SNPs are related to concomitant psoriatic arthritis, and to investigate the toxicity and hepatotoxicity of MTX in patients with psoriasis in the Han Chinese population, visit www. mh. com/growton. PsA-positive patients with PsA were more prevalent than those with PsO and healthy controls, according to the rs1801133 CC genotype. Patients with the rs1801133 TT genotype and others with the CT and CC genotype were significantly higher in MTX patients compared to those with the rs1801133 RT genotype. PsA patients with abnormal liver function were more prevalent in CT+TT genotypes than in those with normal liver function. Conclusions This report found no evidence for MTHFR polymorphism association with PsA risk, as well as the safety and hepatotoxicity of the low-dose MTX in the Chinese population.

Source link: https://doi.org/10.3389/fmed.2022.869912


Protective Effect of Selenium against Methotrexate Induced Hepatotoxicity

Objectives: The main aim of the research is to determine the protective effects of selenium against methotrexate-induced hepatotoxicity in mice. Sigma Chemical Co. produced Selenium and all chemical reagents of analytical grade. The dose of MTX diluted in water 50% 1ml/kg of body weight of Mice was used twice a week for six weeks to produce hepatocellular injury caused by liver function tests. Results: In all groups receiving MTX for the induction of hepatic injury, the serum enzymes and significant decrease in total protein were found. Mice receiving combination therapy of selenium and MTX results in a time course return to normalcy. Conclusion: The biochemical analysis of serum indicated that selenium supplementation in MTX-treated rats resulted in a reduction in the pesticide's toxic effects, which was confirmed by the biochemical analysis of serum.

Source link: https://doi.org/10.47191/ijcsrr/v5-i4-09

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions