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Method for DNA Detection - Europe PMC

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Last Updated: 25 January 2022

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Ni (II) detection by 2-amino-5-substituted-1,3,4-oxadiazole as a chemosensor using photo-physical method: Antifungal, antioxidant, DNA binding, and molecular docking studies.

By UV-visible and fluorescence spectroscopic experiments, the minimum concentration of Ni 2+ ions with LOD was found to be 9. 4 million, with sensing capability investigated towards Ni 2+ ion with binding constant 1. 04 x 10 5 over the other acceptable metal cations. The stoichiometry ratio of Ni 2+ ion vs. oxadiazole derivative 2 is 2:1, according to Job's plotting method. In addition, the anti-fungal activity of oxadiazole derivative 2 was also tested against fungal strains by broth dilution and disc diffusion techniques.

Source link: https://europepmc.org/article/MED/34986516


A field-deployable method for single and multiplex detection of DNA or RNA from pathogens using Cas12 and Cas13.

For some Cas nucleases, trans-cleavage activity triggered by CRISPR/Cas-mediated cis-cleavage on target nucleic acid recognition has been investigated for diagnostic purposes. We harnessed and characterized RfxCas13d as an additional CRISPR/Cas nucleic acid detection device here. We successfully extracted DNA from Fusarium graminearum and Fusarium verticillioides and RNA from rice black-streaked dwarf virus in crude extracts prepared in the field using this technology. This system could be expanded to other crop pathogens, including those that don't have a detection system and have metabolite profiles that make detection difficult.

Source link: https://europepmc.org/article/MED/34962615


A simple PCR-RFLP method for identification and detection of medicinal toad and its crude drug even with serious degradation of DNA.

It's difficult to identify the medicinal toads and related crude medications due to Bufo species' highly similar morphological and chemical composition of the secretions of venom glands and their derived commercial crude drugs, as well as the high similarity in chemical composition of the secretions of venom glands. According to the sequences of amplified cyt-b regions digested by specific restriction enzymes NcoI, EcoRV, and BstXI, respectively, the 23 specimens from three medicinally used Bufo species, B. bufo gargarizans, B. melanostictus, B. melanostictus, and B. raddei were clearly divided into three groups, which could be digested by specific restriction enzymes All nine samples were identified as B. bufo gargarizans, and then's specific PCR-RFLP technique was then used to find 9 samples of industrial crude drugs with significant DNA loss, and then the exact PCR-RFLP technique was also used to find 9 samples of commercial crude drugs even with significant DNA loss.

Source link: https://europepmc.org/article/MED/34864355


Herpes simplex virus DNA testing by a loop-mediated isothermal amplification method for accurate clinical diagnosis and detection of mucosal viral shedding.

Also, although asymptomatic HSV-positive individuals die virus, the exact time of symptoms and viral shedding is uncertain. We collected 445 samples from 211 patients who visited our clinic with suspected herpetic lesions or non-symptomatic volunteers to investigate clinico-laboratorial correlation and whether HSV can be detected in the oral cavity during symptomatom. The LAMP technique found HSV DNA almost as sensitively as PCR. In 54% of HSV DNA samples from the perioral/oral region, a Positivity for HSV DNA was discovered in 54% of specimens. The HSV DNA was found only from lesions local to the perioral skin and/or the dry, vermillion portion of the lip; no HSV DNA was found in immunocompetent patients with lesions restricted to the oral mucosa except primary infection. In 2. 7 percent of samples, HSV was detected in the oral mucosa; all of these patients had either primary infection or immunosuppressed;.

Source link: https://europepmc.org/article/MED/34767275

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions