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Mutagenesis studies amongst oxidosqualene cyclases have shown the impact of amino acid residues on rearrangements during substratum cyclization: loss of catalytic activity, stablizing, rearrangement control or specificity altering. In today research study, friedelin synthase from Maytenus ilicifolia was expressed heterologously in Saccharomyces cerevisiae. Site-directed mutagenesis studies were executed by replacing phenylalanine with tryptophan at position 473, methionine with serine at position 549 and leucine with phenylalanine at setting 552. For this reason, these data revealed that methionine 549 and leucine 552 are important residues for the function of this synthase.
Source link: https://doi.org/10.3390/molecules26226806
Cobalamin-independent methionine synthase in M. alba plant pollen has been confirmed to be among the major irritants for some patients living in Istanbul. The IgE binding sensitivity of rMor a 2 created for the very first time was examined and some structural features were explored by in silico techniques to better comprehend its immunogenicity. This is the first record of the production of recombinant pollen irritant in S. pombe. After the purification, immunoreactivity of rMor a 2 was validated by immunoblotting making use of sera of client allergic to M. alba pollen. Besides, B-cell epitopes of rMor a 2 were forecasted using different bioinformatic devices, particularly Bioinformatics Predicted Antigenic Peptides, BepiPred 2. 0 and Immune Epitope Database whereas T-cell epitopes were approximated utilizing NetMHCIIpan-3. 2 and NetMHCII 2. 3 servers. Conclusions These searchings for might help the usage of rMor a 2 in the medical diagnosis and treatment of allergic diseases connected with M. alba and/or MetE.
Source link: https://doi.org/10.5114/aoms/136322
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