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Methionine Sulfoxide - OSTI GOV

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Last Updated: 13 May 2022

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Novel mechanism for scavenging of hypochlorite involving a periplasmic methionine-rich peptide and methionine sulfoxide reductase

In several habitats, reactive chlorine species defense systems are vital for bacterial growth. These chemicals are also obtained naturally by photochemical reactions of natural organic matter and in vivo by the mammalian immune system in reaction to invaded microorganisms in comparison to bleaching. In comparison to the anthropogenic use of RCS in the form of bleach, these compounds are also produced in vivo by the mammalian immune system in response to invasive microorganisms. We investigated Azospirum strain PS, which produces periplasmic RCS as an intermediate of perpetrolate respiration, in order to gain insight into bacterial RCS defense mechanisms. An RCS response was found by RCS involving a sensitized sigma/anti-sigma factor system, a soluble hypochlorite-scavenging methionine-rich periplasmic protein, and a putative periplasmic methionine reductase. Our results showed that SigF was specifically activated by RCS and started the transcription of a small regulon centering around yedY1 and mrpX. Despite not being restricted by SigF, a yedY1 log was found to have a similar fitness to yedY1. yedY2's synergy with the SigF regulon was shown by Markerless deletions. The MrpX in the periplasm's results indicate a mechanism that involves hypochlorite scavenging by sacrificial oxidation of the MrpX. MrpX is regenerated by YedY's methionine sulfoxide reductase activity, which has led to the Regeneration of MrpX. We were able to find a new defense mechanism based on RCS scavenging by a scathing reaction with a sacrificial methionine-rich peptide and redox recycling via a methionine sulfoxide reductase.

Source link: https://www.osti.gov/biblio/1215524

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions