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Methionine Sulfoxide - Europe PMC

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Last Updated: 13 May 2022

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Chalcogen-based ratiometric reversible BODIPY redox sensors for the determination of enantioselective methionine sulfoxide reductase activity.

Many common disorders are related to degenerative changes in cells as a result of increased concentrations of reactive oxygen species. BODIPY derivatives are introduced here as reversible redox sensors for ROS and enzymatic redox reactions.

Source link: https://europepmc.org/article/MED/35543358


Tomato methionine sulfoxide reductase B2 functions in drought tolerance by promoting ROS scavenging and chlorophyll accumulation through interaction with Catalase 2 and RBCS3B

Reactive oxygen species are naturally present in aerobic organisms as by-products of common metabolism and as a result of defense and evolution. Two target proteins of tomato MsrB2, catalase 2 and the Rubisco small subunit RBCS3B were identified in this research. By contrast, overexpression of SlMsrB2 in tomato reduced ROS accumulation and enhanced drought tolerance, which resulted in reduced ROS accumulation and enhanced drought tolerance. SlMsrB2 interacts with CAT2 and RBCS3B in vitro and in planta, according to a protein interaction analysis. By virus-induced gene silencing, RNAi and RBCS3B induced CAT2 gene silencing, resulting in the growth of pale green leaves and increased ROS accumulation in tomato plants.

Source link: https://europepmc.org/article/MED/IND607663272


The Fused Methionine Sulfoxide Reductase MsrAB Promotes Oxidative Stress Defense and Bacterial Virulence in Fusobacterium nucleatum.

The response regulator ModR regulates a large regulon that includes trx, cdA, and various metabolic genes, according to a comparative transcriptome review. msrAB locus mutants, including msrAB msrAB, are susceptible to reactive oxygen species and ineffective in adhesion/invasion of colorectal epithelial cells. The MsrAB system, which is tightly controlled by the two-component system ModRS, shields fusobacteria against oxidative damage in immune cells and confers virulence by allowing attachment and invasion of multiple target tissues. IMPORTANCE F. nucleatum colonizes many human organs, including oral cavity, placenta, and colonization, according to the author. It's unclear how this obligate anaerobe maintains oxidative stress in host immune cells. The msrAB mutant was unable to colonize placenta and spread to amniotic fluid in a murine model, and was unable to induce preterm birth.

Source link: https://europepmc.org/article/MED/35420473


Protective Effects against the Development of Alzheimer's Disease in an Animal Model through Active Immunization with Methionine-Sulfoxide Rich Protein Antigen.

Acidic oxygen species that may lead to methionine oxidation can cause Alzheimer's disease's brain. Oxidation of beta-amyloid's sole methionine and possibly methionine residues of other extracellular proteins may be one of the first events contributing to the toxicity of A and other proteins in vivo. We immunized transgenic AD mice at 4 months of age with a recombinant methionine sulfoxide-rich protein from Zea mays in the current study. The antigen-injected mice had the following phenotypes at ten months of age: improved short and long memory skills; reduced A burden and MetO accumulations in astrocytes in hippocampal and cortical regions; and enhanced antioxidant activity in the same brain regions as the control mice; and improved antioxidant activity in the same brain regions; and improved antioxidant capabilities in the same brain regions.

Source link: https://europepmc.org/article/MED/35453459


An Intramolecular Charge Transfer-Förster Resonance Energy Transfer Integrated Unimolecular Platform for Two-Photon Ratiometric Fluorescence Sensing of Methionine Sulfoxide Reductases in Live-Neurons and Mouse Brain Tissues.

Oxidative stress in organisms leads to a variety of diseases, including tumors and neurological disorders, while methionine sulfoxide reductases may act as an antioxidant and self-repair mechanism in protein redox cycles. A novel unimolecular platform integrating the intramolecular charge transfer and Förster resonance energy transfer dual mechanisms was built in Herein. A synergistic ICT-FRET activation process was developed for the electron-withdrawing sulfoxide moiety in the probe to an electron-donating sulfide group, leading to a ratiometric fluorescence response toward Msrs with high selectivity, sensitivity, and precision. Moreover, two-photon microscopic imaging of several areas in Alzheimer's disease mice brains revealed a down-regulated Msrs level in aging brains, particularly in the hippocampus region, which may lead to an increase of AD pathogenesis due to neurovascular and self-repair ability.

Source link: https://europepmc.org/article/MED/35412308


Metabolic benefits of methionine restriction in adult mice do not require functional methionine sulfoxide reductase A (MsrA).

Methionine metabolism is directly modulated by the essential amino acid methionine, which is used in several biological applications and limited its availability in the diet. We investigated the role of the ubiquitously expressed methionine repair enzyme methionine sulfoxide reductase A in mice in mice's metabolic benefits. We investigated the extent to which MsrA is required for metabolic reactions of MR in adult mice using mice that lack MsrA. In addition, females had blunted MR responses regardless of MsrA status, relative to males. Overall, our results show that MsrA is not required for the metabolic benefits of MR in adult mice.

Source link: https://europepmc.org/article/MED/35332198


Methionine sulfoxide reductase A in human and mouse tissuesisresponsible for sulindac activation, makingalarger contribution than the gut microbiota.

We investigated whether the human orthologue MSRA catalyzes the sulindac reduction reaction in E. coli, since sulindac is expected to be reduced by methionine sulfoxide reductase reduction reaction. We discovered that sulindac reductase activity in a recombinant human MSRA has a similar Km value as tissue cytosols. MSRA's contribution to the sulindac reductase activities in each tissue cytosol was estimated to be almost 100% by using the relative expression factor. AUC ratio of sulindac sulfide to sulindac dropped by 31% in mice by antibiotic therapy, resulting in a 41% decline in the AUC ratio of sulindac sulfide to sulindac, meaning that tissue MsrA is responsible for 69% in the body. Methionine sulfoxide reductase A expressed in tissues stimulates sulindac with a greater effect than gut microbiota in body.

Source link: https://europepmc.org/article/MED/35279645


Are Methionine Sulfoxide-Containing Proteins Related to Seed Longevity? A Case Study of Arabidopsisthaliana Dry Mature Seeds Using Cyanogen Bromide Attack and Two-Dimensional-Diagonal Electrophoresis.

Many studies have highlighted the role of protein oxidation in seed longevity, particularly those relating to the oxidation of methionine residues to methionine sulfoxide in proteins in recent years. On the carboxyl side of reduced Met residues, CNBr treatment of proteins results in the non-enzymatic hydrolysis of peptide bonds. In an organic one and in an oxidized one, the Arabidopsis thaliana seed protein extract was applied to the Arabidopsis thaliana seed protein extract in a control condition and in an oxidized one. Met residues from twenty-four reactions in 19 proteins characterized by mass spectrometry were discovered to be surface exposed in these proteins. We found amino acid residues that could be converted by oxidation or phosphorylation in Met's three-dimensional environment, which suggest a potential interaction between Met oxidation and other protein modifications. The identification of the proteins that had been oxidatively modified in Met residues led to the finding that MetO-containing proteins are closely linked to seed longevity.

Source link: https://europepmc.org/article/MED/35214905

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions