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Methicillin-resistant Staphylococcus - Astrophysics Data System

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Last Updated: 02 May 2022

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Antibody-mediated enhancement of community-acquired methicillin-resistant Staphylococcus aureus infection

Strains deleted for the pvl genes in a low-inoculum murine skin absces model that includes a foreign body at the infection site were more effective within absces than isogenic PVL+ strains. Mice exposed antibody to PVL and then infected with seven different PVL+ strains had elevated bacterial counts in absces, which was also higher than mice that were not given nonimmune serum. Antibody to PVL had no effect on MRSA strains that did not produce PVL. In vitro, antibody to PVL-inactivated PMNs, incapacitated PVL-mediated activation of PMNs, raising PVL+ MRSA virulence by the interception of PVL-mediated intrinsic immune responses. Given the high prevalence of primary and recurrent MRSA infections in humans, it is likely that antibodies to PVL could have contributed to host susceptibility to infection.

Source link: https://ui.adsabs.harvard.edu/abs/2010PNAS..107.2241Y/abstract


Transmission of Methicillin-Resistant Staphylococcus aureus to Human Volunteers Visiting a Swine Farm

Human health and the health care system are of utmost concern because of the potential for human health and the health care industry. After a short-term stay in a swine farm, the aim was to determine the incidence and duration of MRSA carriage in human volunteers. In four trials, the experimental trial included 34 human volunteers staying 1 h in a MRSA-positive swine farm. Positively related to personal exposure to airborne MRSA and farm work involving pig contact and negatively related to smoking. No association was established between MRSA carriage and face touching behavior, nasal methicillin-susceptible Staphylococcus aureus carriage, age, or gender. The rise in human MRSA carriage among the volunteers with pig contact seems to be linked to an increasing abundance of airborne MRSA of the surrounding air, rather than directly related to physical contact with pigs. IMPORTANCE IN A swine farm The experimental strategy made it possible to elucidate the contributions of airborne MRSA levels and farm work to nasal MRSA carriage in a swine farm. The MRSA burden in the nose was most likely negligible due to the fact that MRSA was in the air at low levels after only 2 hrs, and measures to minimize the number of airborne MRSA or the use of face masks could potentially minimize nasal contamination.

Source link: https://ui.adsabs.harvard.edu/abs/2017ApEnM..83E1489A/abstract


From the Cover: Patient sharing and population genetic structure of methicillin-resistant Staphylococcus aureus

We investigated whether the proportion of shared patients between hospitals was related to geographic similarity of MRSA strains from both hospitals. Multivariate linear regression analysis of 30 of 32 hospitals in Orange County, California, revealed that for every twofold increase in the number of patients shared between two hospitals, there was a 7. 7% decrease in genetic heterogeneity among the hospitals' MRSA populations. These results indicate that coordinated efforts among hospitals that share large numbers of patients may be synergistic to prevent MRSA outbreaks.

Source link: https://ui.adsabs.harvard.edu/abs/2012PNAS..109.6763K/abstract


From the Cover: Methicillin resistance in Staphylococcus aureus requires glycosylated wall teichoic acids

Staphylococcus autus peptidoglycan is highly functionalized with anionic polymers, especially wall teichoic acids. Following the tarS deletion, we discovered that methicillin-resistant S. aureus is sensitive to reticams. Unlike strains who are entirely devoid of WTAs and lactams,'tarS strains, have no growth or cell division defects, and are also sensitive to -lactams. Because neither -O-GlcNAc nor -O-Glucose changes can provide protection, the resistance phenotype requires a highly targeted chemical modification of the WTA backbone, namely the -O-GlcNAc residues.

Source link: https://ui.adsabs.harvard.edu/abs/2012PNAS..10918909B/abstract


The prevalence of virulence determinants in methicillin-resistant Staphylococcus aureus isolated from different infections in hospitalized patients in Poland

Staphylococcus aureus, which is resistant to drugs, is responsible for difficult-to-treat infections. The presence of 19 virulence genes in 120 MRSA isolates isolated from hospitalized patients and the genetic links of these isolates were investigated. In 100, 70. 8 percent, and 94. 2% isolates, respectively, splA, splE, and sspA were discovered in 100%, 78%, and 94. 2% isolates. In 75% isolates, the tst gene, which encodes toxic shock syndrome toxin-1, was present. The cna, map/eap, and tst genes were the most common in wound isolates, but they were less common in blood isolates. The identification of MRSA's virulence factors is crucial for determining pathogen transmission rate and disease growth.

Source link: https://ui.adsabs.harvard.edu/abs/2022NatSR..12.5477K/abstract


Human Urine Alters Methicillin-Resistant Staphylococcus aureus Virulence and Transcriptome

However, it is also important to recognize MRSA pathophysiology in the urinary tract because MRSA isolate in urine samples often precedes potentially lethal MRSA bacteremia. We exposed three S. aureus clinical isolates, including two MRSA strains, to human urine for 2 h to determine gene expression and virulence characteristics. Human urine rapidly alters the adhesion to human bladder epithelial cells and fibronectin, hemolysis of sheep red blood cells, and surface hydrophobicity in a staphylococcal strain-specific manner, according to in vitro virulence assays. Our findings, in summary, reveal important information into how human urine specifically and quickly changes MRSA physiology and aids MRSA survival in the nutrient-limiting and hostile urinary microenvironment. However, it is vital to know MRSA pathophysiology in the urinary tract because MRSA isolation in urine samples often precedes potentially lethal MRSA infections.

Source link: https://ui.adsabs.harvard.edu/abs/2021ApEnM..87E.744P/abstract


A Factor H-Fc fusion protein increases complement-mediated opsonophagocytosis and killing of community associated methicillin-resistant Staphylococcus aureus

Staphylococcus aureus employs a variety of immune strategies to defy host defenses, including the complement system, which is a component of innate immunity that is vital to fighting bacterial infections. S. aureus binds the complement regulator factor H via surface protein SdrE, which leads to impairment of complement. Compared to Fc-control proteins and FH-Fc, S. aureus bounding was significantly greater FH-Fc, and FH-Fc performed with serum FH for S. aureus binding. Both C3b and iC3b have increased C3-fragment opsonization in S. aurus, as well as the increase of the anaphylatoxin C5a's generation. Polymorphonuclear cells in 5 and 10% serum contributed to significant S. aureus killing by polymorphonuclear cells. This research shows that FH-Fc fusion proteins have the ability to reduce the protective effects of bound serum FH rendering S. aureus' host immune system.

Source link: https://ui.adsabs.harvard.edu/abs/2022PLoSO..1765774S/abstract


Methicillin-Resistant Staphylococcus aureus Strains in Swiss Pigs and Their Relation to Isolates from Farmers and Veterinarians

MRSA prevalence in Swiss pigs has risen from 2% in 2009 to 44% in 2017, according to our study. In addition, we may have found that pig spa t011 strains cluster separately and are thus less likely to colonize humans than are pig spa t034 strains. The latest report looked at the course of MRSA infection in Swiss livestock since 2009, with a special emphasis on pigs and screening of veterinarians and farmers. MRSA in Swiss pigs increased from 2% in 2009 to 44% in 2017, according to porcine t034 strains. The same could be said for spa t011 strains from horses and veterinarians. A significant number of additional resistance genes had been discovered in spa t034 strains, and two strains had inherited the immune evasion cluster, and two strains had the immune evasion cluster. However, all but one of the pig spa t011 strains remained in a separate group. Thus, the rise in pig spa t011 strains does not necessarily refer to humans. MRSA prevalence in Swiss pigs has increased from 2% in 2009 to 44% in 2017, according to our study. We were able to show a close relationship between farmer and pig strains as well as veterinarian and horse strains, indicating that the respective animals are a potential source of human colonization. In addition, we could find that pig spa t011 strains cluster separately and are likely to colonize humans more quickly than are pig spa t034 strains.

Source link: https://ui.adsabs.harvard.edu/abs/2020ApEnM..86E1865K/abstract


Antimicrobial peptide GL13K immobilized onto SLA-treated titanium by silanization: antibacterial effect against methicillin-resistant Staphylococcus aureus (MRSA)

Implant failure is primarily due to infection, and the incidence of drug-resistant bacterial infections has increased in recent years. Antimicrobial peptides have demonstrated superiority over conventional antibiotics in inhibiting drug-resistant bacteria in inhibiting drug-resistant bacteria. We combined the antimicrobial peptide, GL13K, with sandblasting and acid-etching-treated titanium, using a silane coupling agent in the present research. The hydrophilicity of the SLA-GL13K coating was determined by water contact angle analysis, and Attenuated total reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy findings confirmed loading of GL13K. The coating has a long-releasing profile, according to the published review. MRSA, E. coli, and S. aurus coatings, as well as S. aurus, demonstrated strong contact- and release-killing capabilities against MRSA, E. coli, and S. aurii. However, Cell Counting Kit 8 analysis and analysis of cell morphology revealed that the SLA-GL13K coating had good cytocompatibility at antibacterial concentrations.

Source link: https://ui.adsabs.harvard.edu/abs/2022RSCAd..12.6918L/abstract


Zinc Resistance within Swine-Associated Methicillin-Resistant Staphylococcus aureus Isolates in the United States Is Associated with Multilocus Sequence Type Lineage

Because czrC and mecA are related to the emergence and distribution of methicillin-resistant S. aureus in swine, it has been suggested that the use of zinc in feed as an antidiarrheal agent could have the ability to contribute to the outbreak and dissemination of methicillin-resistant S. aureus in pigs. We review the prevalence of the czrC gene and phenotypic zinc resistance in swine-associated LA-MRSA ST5 isolates, MRSA ST5 isolates from humans without swine contact, and U. S. swine-associated LA-MRSA ST398 isolates from humans. zinc resistance in MRSA ST5 isolates from humans with no swine contact and swine-associated LA-MRSA ST398 isolates, as well as prevalences of zinc resistance in other LA-MRSA ST398 isolates were significantly lower than those from previous studies describing zinc resistance in other LA-MRSA ST398 isolates.

Source link: https://ui.adsabs.harvard.edu/abs/2017ApEnM..83E.756H/abstract

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions