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Compared to chemical or physical process, the desire for green synthesis of metallic nanoparticles has heightened. Silver nanoparticles were produced in a non-cellular synthesis method using dried orange peel extract here. Our findings showed anticancer activity of biogenic silver NPs against the B16 melanoma cell line with an IC50 value of 25 g/ml. In addition, the AgNPs demonstrated potent antibiofilm activity against MRSA strains, with the percent biofilm destruction rate approaching 80%.
Source link: https://europepmc.org/article/MED/35368519
An improved understanding of the differences among S. aureus strains that are sensitive and resistant to antibiotics could help determine the resistant phenotype and identify new antimicrobial targets. This study was intended to compare general characteristics of lipid profiles among clinical strains of S. aureus sensitive and resistant to antibiotics. Both cell wall thickness and cell surface charge were also compared. Methods In the absence of antibiotics, five methicillin resistant S. aureus strains were tested individually and as MSSA and MRSA groups. In all ten strains of S. aureus, twenty-two lipid species were found. The thickness of cell wall thickness was determined among strains, but not between MSSA and MRSA. Conclusions This research found differences in membrane lipids between antibiotic sensitive and antibiotic resistant clinical strains of S aureus, which may have an effect on resistance mechanisms related to cell membrane structure and fluidity.
Source link: https://europepmc.org/article/MED/35277120
However, the Chinese MRSA ST22's genetic characteristics and evolution are yet to be determined, although the genetic structure and evolution of the Chinese MRSA ST22 remain to be determined. Herein, we performed comparative genomics analysis of 12 ST22 community-associated MRSA isolates from China, based on 9 Chinese ST22 CA-MSSA isolates and 284 ST22 genomes from international sources to determine the genetic structure and potential transmission of MRSA ST22 strains is isolated in China. The Chinese subclade appears to have emerged around 2006, rather than spread from other regions, as shown by differences in the lukSF - PV and tst-1 carriages. Virulence assays found that the ST22-MRSA clone was highly infectious, with increased or similar virulence potential as MSSA's MRSA predecessors and the outbreak USA300 and ST22-MSSA. Previous work has listed the genetic structure and virulence potential of Chinese transmission of ST22 strains originating from EMRSA-15 and Gaza clone; however, the genetic structure and virulence potential of Chinese dissemination of ST22 strains are still limited; To determine the genetic characteristics and potential transmission of MRSA ST22 strains isolated from China, we conducted a comprehensive review of global ST22 strains. The ST22-SCC mec V may have originated from the native ST22-MSSA clone rather than spread from other regions, according to the high virulence of the Chinese ST22 strains, owing to the high expression of agr based on the results of virulence assays.
Source link: https://europepmc.org/article/MED/35138159
Since they are able to resist methicillin and commonly produce biofilms, Methicillin-resistant Staphylococcus epidermidis bacteria have been recognized as genuine pathogens. We investigated melittin's antibiofilm activity against biofilm-producing MRSE strains in this research. For the antimicrobial agents tested, minimum biofilm inhibitory concentration, minimum biofilm inhibition degree, and minimum biofilm eradication concentration were determined, as well as fractional biofilm preventive, inhibitory-, and eradication concentrations. Human embryonic kidney cells and Red Blood Cells were tested on human embryonic kidney cells and Red Blood Cells, respectively. Based on these results, melittin and conventional antibiotics may have been combined in a drug therapy or prevention of biofilm-associated MRSE infections.
Source link: https://europepmc.org/article/MED/35123230
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