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Methicillin-Resistant Staphylococcus Aureus - DOAJ

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Last Updated: 02 May 2022

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Everybody nose: molecular and clinical characteristics of nasal colonization during active methicillin-resistant Staphylococcus aureus bloodstream infection

St. aureus, a common commensal pathogen and a common cause of bacteremia, has been studied, showing that nasal and blood isolates from single patients match more than 80% of the time. MRSA expansion was based on a single colony culture from the blood and an average of six colonies from the nares. MRSA bloodstream infection, 53 were swabbed, 53 were confirmed, and 37 were colonized with MRSA in the anterior nares, over an 11-month period. In 95 percent of the cases, spa types and clonal complexes were also present in the blood. In the nares, 11% of patients had more than one clone of MRSA. Conclusions The molecular epidemiologic landscape of colonization in the context of invasive disease is varied, and determining the interplay between colonization and invasive disease is crucial in combating invasive MRSA disease is crucial to combating infectious MRSA disease.

Source link: https://doi.org/10.1186/s12879-022-07371-w


Metal nanoparticles functionalized with nutraceutical Kaempferitrin from edible Crotalaria juncea, exert potent antimicrobial and antibiofilm effects against Methicillin-resistant Staphylococcus aureus

For the first time, C. juncea extract and kaempferitrin were used for the first time in the creation of silver and copper nanoparticles. KF@AgNPs and KF@CuNPs' efficacy against biofilm synthesis and planktonic growth on methicillin-resistant Staphylococcus aureus, as well as potential mechanisms has been investigated. Cu after continued treatment with KF@CuNPs in the presence of MRSA was determined by an Alizarin red test, indicating that the CuNPs started the antibacterial action by the CuNPs itself. With Crystal violet assay, Fluorescent live dead imaging and SEM analysis revealed a 60% reduction in biofilm formation at KF@AgNPs and KF@CuNPs, showing that both the NPs had similar kill kinetics to slow the pathogen and imaging. According to KF@AgNPs and > two fold for KF@CuNPs, the colony count from the in vivo infection zebrafish model in the treatment program decreased by 1. 8 fold for KF@AgNPs and > two fold for KF@CuNPs.

Source link: https://doi.org/10.1038/s41598-022-11004-2


Sanchen powder extract combined with vancomycin against methicillin-resistant Staphylococcus aureus

The aim was to investigate the effects of Sanchen powder extract mixed with vancomycin on methicillin-resistant Staphylococcus aureus planktonic cells, biofilms, and virulence factors. Methods: The herbs in Sanchen powder were extracted separately with 50 percent ethanol. By ultraviolet visible-vis spectrophotometry and high-performance liquid chromatography, in the extraction of artificial cattle bezoar and safflower, the amount of hydroxysafflower yellow A and cholic acid was determined by Then, but the presence of hydroxysafflower yellow A and cholic acid in the extract of artificial cattle bezoar and safflower was determined by ultraviolet visible-vis spectrophotometric Using a 2,3-bis-tetrazolium5-carboxanilide reduction assay and scanning electron microscopy, the effects of ESCP and vancomycin on MRSA were assessed by comparing its biofilm viability. Results: The cholic acid content of the artificial C. bovis extract was 7. 34 mg/g, and the C. tinctorius extract's HSYA content was 9. 18 mg/g. This report is the first to show that ESCP and vancomycin may reduce coagulase and MRSA embedded in mature biofilms, as well as a promising MRSA therapy.

Source link: https://doi.org/10.1016/j.jtcms.2022.03.002


Antimicrobial resistance pattern of methicillin-resistant Staphylococcus aureus isolated from sheep and humans in Veterinary Hospital Maiduguri, Nigeria

Infections caused by antibiotic-resistant S. aureus cause significant health issues in the general population. This research was designed to determine the presence of MRSA in both locally healthy and sick sheep brought to the hospital as well as veterinarian staff and students on clinical attachment in the hospital. Materials and Methods: For the isolation of MRSA, a total of 200 nasal swab samples were obtained aseptically from sheep and humans. MRSA was isolated using the Clinical Laboratory Standard Institute's guideline and confirmed by plating onto Oxacillin Resistance Screening Agar Agar. The MRSA isolates' antimicrobial susceptibility pattern was determined using the disk diffusion technique against 12 commonly used antimicrobial agents. Results: The total rate of nasal carriage of S. aureus and MRSA was found in sheep and humans at 51% and 43% respectively. Both male and female sheep had 18% and 8% prevalence, respectively, while 9% and 8% were for male and female human samples, respectively. By MRSA isolates from humans, the antimicrobial susceptibility test revealed 100% resistance to OX, cefoxitin, oxytetracycline, cephazolin, and penicillin-G. The MRSA isolates from sheep were 100% resistant to the same formulation of medications used to isolate human MRSA isolates and were equally vulnerable to gentamicin, imipenem, LZD, ciprofloxacin, NOR, and ERY. MRSA was discovered in sheep and humans from the Veterinary Hospital in Maiduguri, according to this report.

Source link: https://doi.org/10.14202/vetworld.2022.1141-1148


Methicillin-resistant Staphylococcus aureus infections in postpartum period

It's unclear if methicillin-resistant Staphylococcus aureus in obstetric populations. Even though MRSA prevention strategies have improved, MRSA infection incidence in pregnant women and neonates has increased in pregnant women and neonates. Both mothers and infants are vulnerable to MRSA infections. MRSA is the most common pathogen responsible for postpartum mastitis. Following delivery, the aim of this study was to identify the most common risk factors for postpartum MRSA infection and determine the incidence of antibiotic-resistant Staphylococcus aureus infections. A literature review was done using PubMed and the following key words were used: MRSA infection in postpartum, “risk factors for postpartum MRSA infection” were used in PubMed. We've included 27 articles from the last 20 years that included rare cases of MRSA infection in postpartum and others that determined the risk factors of this disease after delivery. MRSA infections seem to be more common among pregnant women colonized with MRSA.

Source link: https://doi.org/10.37897/RJID.2021.4.3


Eradication of Biofilm-Mediated Methicillin-Resistant Staphylococcus aureus Infections In Vitro: Bacteriophage-Antibiotic Combination

Phage+antibiotic mixtures may be more effective on biofilms than any other form of agent alone, but it is impossible to determine which PAC regimens will be most efficient. We performed biofilm time-kill experiments using various combinations of phage Sb-1 with clinically relevant antibiotics to determine a method for screening PAC combinations against Staphylococcus aureus biofilms. We also saw less PACs against DNS-VISA mutants in our experiments with isogenic strain pairs than their respective parents. We found anti-biofilm synergy between Sb-1 and DAP in the phage+daptomycin regimen in the VISA-DNS strain 8015 and DNS strain 684. We did not observe any bacterial resensitization to antibiotics following therapy, but phage adherence was avoided after exposure to PAC regimens for all tested strains. The introduction of bacterial membrane vesicles by various treatment regimens seemed to be either unaffected or reduced by the various treatment regimens. Interestingly, phage yields from such biofilm experiments were higher than those from similar planktonic experiments, meaning that Sb-1 might be more widely propagated on biofilm. However, no information is known about how phages and their hosts function in the biofilm environment or how different phage+antibiotic combinations affect biofilms in comparison to the planktonic state of bacteria, although scattered studies show that phage+antibiotic synergy occurs more readily under biofilm-like conditions. phage Sb-1 can infect MRSA strains both in biofilm and planktonic states, as well as suggested PAC regimens that are worthy of further investigation as adjuncts to antibiotics, according to our findings.

Source link: https://doi.org/10.1128/spectrum.00411-22


Role of Extracellular DNA in Dalbavancin Activity against Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilms in Patients with Skin and Soft Tissue Infections

The aim of the study was to determine the efficacy of various antibiotics on biofilm-producing MRSA isolate from patients with SSTI. Patients with SSTI were found with a total of 32 MRSA strains. Planktonic and biofilm growth was determined at the MIC and minimal biofilm eradication concentrations. Of the MRSA strains, 85. 5% were excellent biofilm designers, while only 12. 5% were poor biofilm developers. In all tested strains, the MBEC90 values for dalbavancin were significantly lower than those of linezolid and vancomycin. We also found that extracellular DNA plays a role in the initial microbial attachment and biofilm formation. The number of eDNA varied among MRSA strains and was much higher in those isolates with high dalbavancin and vancomycin tolerance, as shown by a yellow line. The relative abundance of eDNA in MRSA biofilms exposed to MBEC90 dalbavancin was higher than in untreated MRSA biofilms and those exposed to sub-MIC90. Overall, dalbavancin was the most effective antibiotic against MRSA biofilms in human serum concentrations. IMPORTANCE Staphylococcus aureus is the most common cause of skin and soft tissue infections worldwide. In addition, methicillin-resistant S. aureus infections are becoming more common in postoperative infections and are responsible for a large number of hospital readmissions and deaths. SSTIs are the most common risk factor due to a higher antibiotic tolerance. The biofilm-producing capacity varied among MRSA strains, according to our report, although major biofilm producers were more prolific than weak biofilm producer strains.

Source link: https://doi.org/10.1128/spectrum.00351-22


Rapid Identification of Methicillin-Resistant Staphylococcus aureus Using MALDI-TOF MS and Machine Learning from over 20,000 Clinical Isolates

The area under the classification model's receiver operating curve was 0. 78 to 0. 88 for the dataset set, which included 20,359 clinical isolates. Compared to UPF0337 protein SACOL1680, which is mainly present in methicillin-susceptible S. autus, the key MRSA feature, m/z 6,590 to 6,599, was identified as a UPF0337 protein SACOL1680 with a lower binding affinity or no docking results. Our MALDI-TOF MS-based machine learning system for rapid MRSA detection can be easily integrated into current clinical workflows and can even help physicians in prescribing the right antibiotic therapy. IMPORTANCE Over 20,000 clinical MSSA and MRSA isolates were collected to create a machine learning system to determine MSSA/MRSA and their markers. MRSA markers were the first discovery and validation of MRSA isolates on the largest scale of clinical MRSA and MRSA isolates collected to date, five separate clinical centers.

Source link: https://doi.org/10.1128/spectrum.00483-22


The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis

After supplementation with cadaverine or Enterococcus faecalis, the key microbiota genus for cadaverine synthesis, these beneficial results were established in aryl hydrocarbon receptor knockout mice and abrogated mice, and mice were obnoxious in aryl hydrocarbon receptor knockout mice and abrogated. In addition, increased cadaverine levels in feces and serum were found in critically ill patients with widespread MRSA infection, as well as chronic MRSA infections, although cadaverine was not present in healthy controls. Concluding trial Registration[http://www. chicontr. org] identifier]ChiCTR1800018646] has been a potential therapeutic target against abdominal sepsis. [ChiCtR1800018646] identifier [ChiCTR1800018646] identifier [ChiCTR1800018646] identifier [ChiCTR1800018646] identifier [B] 18346]: In addition, cytogenetic [B] cytomyo[/B] cytotr] cyto-[Chr].

Source link: https://doi.org/10.3389/fmicb.2022.802409


In vitro Antimicrobial Activity and the Mechanism of Berberine Against Methicillin-Resistant Staphylococcus aureus Isolated from Bloodstream Infection Patients

paraphrasedoutput:Methods: To investigate the antimicrobial activity of berberine and the manner by which it combats methicillin-resistant Staphylococcus aureus strains isolated from bloodstream infections. Cell wall alterations and cell membrane integrity were measured by confocal laser scanning microscopy and electron microscopy to determine the effect on cell morphology, as shown by our results. MRSA was remarkably effective against MRSA at MIC values ranging from 256 to 64 mg/l;L1 for different MLST types.

Source link: https://doaj.org/article/121528697ff24217983861bc3d86f5eb

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions