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Scope: The present research focused on MRSA phage species and its antibiotic susceptibility pattern in wounds. Staphylococcus aureus cultured from various wounds is found in this study by inoculating on Blood agar, MacConkey agar, and Nutrient agar, which is then processed further by necessary biochemical testing and tube coagulase tests. MRSA of 23. 9 percent, Methicillin-sensitive staphylococcus aureus of 76. 08%, and the most common phage type in MRSA and MSSA were phage III. Conclusion: Phage typing may be a very useful diagnostic device in the future.
Source link: https://doi.org/10.20546/ijcmas.2022.1102.025
In the general population, antibiotic-resistant S. aureus infections cause significant health problems. This research sought to determine the presence of MRSA in both newly healthy and sick sheep donated to the hospital by the veterinary hospital, as well as veterinary staff and students on clinical attachment in the hospital. The samples were processed by properly transporting them to the laboratory, where they were propagated in mannitol salt agar at 37°C for 24 hrs to identify S. aureus. MRSA was isolated using a Clinical Laboratory Standard Institute guideline and confirmed by plating onto Oxacillin Resistance Screening Agar Agar. Using the disk diffusion method against 12 commonly used antimicrobial agents, the MRSA isolates' antimicrobial susceptibility pattern was established. S. aurantus and MRSA were found to be 51% and 43% in sheep and humans, respectively. MRSA prevalence in male and female sheep was 18% and 8%, respectively, with 9% and 8% for male and female human samples. MRSA isolates from sheep were 100% resistant to the same drugs used for human MRSA isolates and were equally vulnerable to gentamicin, imipenem, LZD, ciprofloxacin, NOR and ERY. MRSA was discovered in sheep and humans from the Veterinary Hospital in Maiduguri, according to this report. To minimize the burdens of MRSA resistance, the reasons for the observed patterns of resistance must be investigated.
Source link: https://doi.org/10.14202/vetworld.2022.1141-1148
Patients with MRSA-infected or infected in the neurosurgical unit between 1993 and 1999 were retrospectively identified from laboratory records. In patients with MRSA that had a longer hospital stay than in all patients treated in the unit, the mean duration of hospital stay was longer in patients with MRSA than in all patients treated in the unit. Methicillin-resistant S. aureus was isolated from the nose in 89 patients, the throat in 79, the perineum in 52, surgical wounds in 16, and multiple sites in 69 patients. MRSA-16 and EMRSA-15 were two common MRSA isolates that were isolated, with epidemic MRSA-16 and EMRSA-15. Infection with MRSA is a common problem in the neurosurgical population, and most cases are hospital-acquired and associated with longer hospital stays. Most patients with MRSA are discharged before the disease is established, raising the likelihood that the disease will spread in the community.
Source link: https://doi.org/10.3171/jns.2003.98.1.0008
Per hospital policies, the 260 patients from this group who were admitted to the intensive care unit underwent MRSA testing based on nasal swab cultures and a analysis of their medical history for previous MRSA infections. These patients were classified as either MRSA positive or MRSA negative, depending on their MRSA coding. The 232 patients admitted to non-ICU nursing units were not eligible for MRSA testing and were classified as unscreened. The authors found that the MRSA-positive, MRSA-negative, and unscreened groups had elevated incidences of postoperative neurosurgical wound infections caused by all pathogens, which were 23. 5, 4. 1, and 1. 3%, respectively. Patients with MRSA were reported to have the following clinical characteristics: male sex in 63%, diabetes in 38. 9%, an immunosuppressed state in 14. 8 percent, and a traumatic injury in 15. 2%. In patients receiving the standard therapy, the incidence of postoperative neurosurgical wound infection in patients receiving MRSA-specific prophylactic antibiotic therapy was 7. 4% relative to 32. 9% in patients receiving the standard therapy. With chlorhexidine cleaning at 3-day intervals, Wound care for ICU patients was standardised for postoperative Days 0–7. In 7 of 11 patients, Wound cultures from neurosurgical site infections in patients with prior MRSA colonization or infection grew MRSA in 7 of 11 patients. To minimize MRSA wound colonization, neurosurgical patients with MRSA colonization or a history of MRSA infections receive targeted perioperative care, including prophylactic antibiotics active against MRSA and postoperative wound care with coatings and chlorhexidine antisepsis to minimize MRSA wound colonization.
Source link: https://doi.org/10.3171/2009.5.jns081589
Infection of an intrathecal pump system is a rare but complicated process that usually results in the pump's removal. In a patient with such a pump who was effectively treated with linezolid without the need for removal of the intrathecal pump, the authors detail the first case of methicillin-resistant Staphylococcus aureus meningitis. If no clinical improvement after a combined intravenous antibiotic therapy regimen of vancomycin and rifampicin resulted in no clinical improvement, that treatment was discontinued and linezolid was administered intravenously as monotherapy. After 1 week of linezolid therapy, blood and CSF cultures were sterile, with a sterile attitude in the wake of 1 week of linezolid therapy. After which the patient was treated with oral linezolid for three months, intravenous therapy was continued for a total of three weeks. These findings support previous findings of the toxicity of linezolid in the treatment of acute infections of the central nervous system caused by multidrug-resistant Gram-positive bacteria, especially postneurosurgical infections.
Source link: https://doi.org/10.3171/jns-07/09/0651
Staphylococcus aureus, a Gram-positive bacterium that can cause life-threatening diseases and certain minor infections in living organisms, is a Gram-positive bacterium that can cause life-threatening diseases and certain minor infections in living organisms. Methicillin-resistant Staphylococcus aureus, a methicillin-resistant bacteria that has emerged as a superbug due to its defiant activity against the antibiotics and medications used to treat major and minor infections. This review discusses the clinical management of MRSA pathogenesis, recent advances in rapid diagnosis, and MRSA-based antimicrobial therapy options for MRSA.
Source link: https://doi.org/10.3390/antibiotics11050606
For the first time, C. juncea extract and kaempferitrin were used to achieve optimum for the synthesis of silver and copper nanoparticles. On methicillin-resistant Staphylococcus aureus and KF@CuNPs, Efficacy of KF@AgNPs and KF@CuNPs against biofilm formation and planktonic growth has been investigated, as well as potential mechanisms. Cu after prolonged therapy with KF@CuNPs in the presence of MRSA was determined by Alizarin red test, indicating that the CuNPs' antibacterial activity was initiated by the CuNPs itself. Both the NPs had similar kill kinetics to reduce the pathogen and imaging with Crystal violet assay, Fluorescent live dead imaging and SEM analysis showed a 60% decrease in biofilm formation at the Sub-MIC concentrations of KF@AgNPs and KF@CuNPs. For KF@AgNPs and KF@CuNPs, the colony count from the in vivo infection zebrafish model in the treatment group decreased by > 1. 8 fold but KF@CuNPs declined by more than two fold.
Source link: https://doi.org/10.1038/s41598-022-11004-2
Lemierre syndrome is an oropharyngeal infection accompanied by bacteremia, thrombosis of the internal jugular vein, and subsequent hematogenic metastasis via septic emboli. In a 5-month-old Saudi boy, we report a case of Lemierre syndrome complicated by lower necrotizing mediastinitis due to a Methicillin-resistant Staphylococcus aureus infection. Pak J Med Sci, Sci.
Source link: https://doi.org/10.12669/pjms.38.5.5442
History Host-microbiota crosstalk has been implicated in multiple host metabolic pathway axes that regulate intestinal barrier function. Mice lacking CYP1A1 caused an altered gut microbiome, a decreased metabolic shift from lysine to cadaverine in the caecal contents and antimicrobial molecule manufacturing, and they were shielded from gut barrier disruption when exposed to MRSA challenge. These beneficial results were confirmed in aryl hydrocarbon receptor knockout mice and abrogated mice following cohousing with CYP1A1A1 KO mice and abrogated after supplementation with cadaverine or Enterococcus faecalis, the primary microbiota genus for cadaverine synthesis. Following MRSA infection, biotic-driven gut dysbacteriosis weakened the survival result and disrupted intestinal barrier function in CYP1A1 KO mice. During persistent MRSA infection, elevated cadaverine levels in feces and serum were found in critically ill patients with gut leakage during persistent MRSA infections, however, cadaverine was not present in healthy controls.
Source link: https://doi.org/10.3389/fmicb.2022.802409
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