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Fresh and novel insights into methane biochemistry, which have been otherwise unobtainable through other methods such as conventional stable isotope determinations and molecular composition measurements of hydrocarbons, have been provided by recently developed methane clumped isotope methods. Our study aims to shed new light on the emergence and migration of methane in naturally occurring gas hydrate deposits, resulting in widespread inquiries regarding the source and migration of methane in naturally occurring gas hydrate deposits. Five shallow marine sediment sites on the eastern margin of the Japan Sea's eastern shore of the region were found, and the bulk of them contain 13 CH 3 D and 12 CH 2 D2 temperatures that reportedly match expected methane formation temperatures. The findings not only show that methane clumped isotope data to detect potential end-members in natural methane samples, but also show that more conventional carbon isotope approaches could significantly underestimate the percentage of thermogenic methane present in global gas hydrate reservoirs. In addition, the role played by gas hydrate dissociation in recent geological events, thanks to advancements in the accuracy of source attribution. The thermogenic methane was present at a marine sedimentary environment at the time when the thermogenic methane was first detected at the time in reconstruction of the paleogeothermal gradient. The estimated growing temperatures of thermogenic end-member can be used to refine the paleo geothermal gradient.
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