Metastasis Microenvironment - Crossref

Helicase-like transcription factor-deletion from the tumor microenvironment in a cell line-derived xenograft model of colorectal cancer reprogrammed the human transcriptome-S-nitroso-proteome to promote inflammation and redirect metastasis v1

The HLTF gene in colorectal cancer cells is more prevalent in men than in women. HLTF's tumor cell gene expression is accompanied by negligible activity in the tumor microenvironment. By direct orthotopic cell microinjection of HLTF+/+HCT116 Red-FLuc cells into the submucosa of the cecum, xenografts were established in control and Hltf-deleted male Rag2-/-IL2rg//- mice. Comparing to controls, the proinflammatory group saw a major shift in CDX metastasis to peritoneal dissemination. In the Hltf-deleted TME, a general S-nitrosylation target and a iNOS-S100A8/A9 site-specific target are both a general S-nitrosylation target and a iNOS-S100A8/A9 site-specific target. TME tumors from Hltf-deleted TME generated 60% of their S-nitroso-proteome, according to all metastatic sites. When the tumor cells expressed HLTF, tumor cells exhibited HLTF, a major role in the pathogenesis of inflammation and related protein S-nitrosylation in primary CDX tumors with spatiotemporal continuity in metastatic progression.

Source link: https://doi.org/10.17504/protocols.io.bs5xng7n

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