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Metalloproteinase - Wiley Online Library

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Last Updated: 23 May 2022

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Loss of Mitochondrial Dynamics Proteins Mitofusin‐2 and Drp‐1 in Myocardial Ischemia‐Reperfusion Injury Is Prevented by Matrix Metalloproteinase‐2 Preferring Inhibitors

According to respective studies, mitochondrial dynamics involving fusion and fission are affected by oxygen regulation by oxygen and fission. MMP 2 is localized at the mitochondrial outer membrane, and MMP2 is localized at the mitochondrial endoplasmic membrane, and we suspect MMP2 will proteolyze Mfn 2 and Drp1. During I/R injury, we therefore investigated whether blocking MMP2 could prevent mitochondrial dynamics proteins loss during I/R injury. Methods Hearts isolated from 3 month old C57BL/6J mice were perfused and exposed to I/R injury in the absence or presence of MMP2 preferring inhibitors ARP100 or ONO 4817 or their vehicle, according to Langendorff. MMP2 activity was determined in the cytosolic fraction as a marker of MMP2 function. Using western blot, Mfn2 and Drp-1 in the mitochondrial fraction were also measured. Using Procleave, an In silico analysis of potential MMP2 cleavage sites was carried out. Results ARP 100 or ONO4817 significantly raised left ventricular developed pressure compared to vehicle-treated I/R hearts. Both mouse Mfn 2 and Drp sequences showed several potential locations that could be in silico target by MMP2. MMP2 can alter mitochondrial dynamics during myocardial I/R injury, according to MMP,2 and Drp1's proteolysis. Inhibition of MMP2 production could prevent cardiac contractile dysfunction in part by preserving these proteins that influence mitochondrial fusion and fission.

Source link: https://onlinelibrary.wiley.com/doi/10.1096/fasebj.2022.36.S1.R2993


Matrix metalloproteinase‐21 promotes metastasis via increasing the recruitment and M2 polarization of macrophages in HCC

However, the roles of MMP-21 in hemofiltrate C–C chemokine remain largely unclear. Although changing MMP21 in HCC cell lines had no effect on cell migration or invasion capabilities in vitro transwell tests, both IHC analysis and in vivo mouse models found that downregulated MMP-21 promoted metastasis had no effect on cell migration or invasion capabilities in vitro transwell experiments, both IHC analysis and in vivo mouse models confirmed that upregulated MMP-21 promoted metastasis. MMP21 boosted macrophage recruitment by increasing CCL-14 levels and promoted macrophage polarization by raising the expression of CSF1 and FGF-1, according to further research.

Source link: https://onlinelibrary.wiley.com/doi/10.1111/cas.15368

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions