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Metalloproteinase - DOAJ

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Last Updated: 23 May 2022

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In vitro and in vivo preclinical venom inhibition assays identify metalloproteinase inhibiting drugs as potential future treatments for snakebite envenoming by Dispholidus typus

The monovalent SAIMR Boomslang antivenom is the only approved treatment for D. typus envenoming. We therefore investigated the in vitro and in vivo preclinical effectiveness of four SVMP inhibitors to neutralize D. venom's effects; the matrix metalloproteinase inhibitors marimastat and prinomastat; and the metal chelators dimercaprol and DMPS. In vitro, the venom of D. typus had an SVMP-driven procoagulant phenotype. The preclinical findings published here reveal that DMPS and marimastat have promise as novel small molecule-based therapeutics for D. typus snakebite envenoming. In preclinical studies, these two drugs have been shown to be safe against Echis ocellatus VICC, and as such, we suggest that marimastat and DMPS could be more explored as potentially useful early intervention therapeutics to broadly treat VICC in sub-Saharan Africa.

Source link: https://doi.org/10.1016/j.toxcx.2022.100118


Hydrocortisone supresses inflammatory activity of metalloproteinase - 8 in carotid plaque

Results: MMP-8 and MMP-9 serum levels were measured in carotid plaque, an organ transplant patient, and MMP-9 were elevated in tissue samples at 1 hour after carotid endarterectomy, relative to the control group. MMP-8 in group Symptomatic patients in Group 1 had lower tissue concentrations of MMP-8 in comparison to the control group. MMP-9 levels and tissue samples of MMP-8 and MMP-9 among symptomatic patients in the control group were found to be in a correlation, especially in symptomatic patients. Conclusion: It was found that before-operative MMP-9 levels and tissue samples of MMP-8 and MMP-9 decreased in symptomatic patients.

Source link: https://doi.org/10.5935/1678-9741.20150034


Changes in salivary matrix metalloproteinase-3, -8, and -9 concentrations after 6 weeks of non-surgical periodontal therapy

Abstract Background In recent years, studies using salivary inflammatory biomarkers for diagnosis and monitoring periodontal disease have gained increasing attention. During all three visits, patients with periodontal disease underwent formal toothbrushing. Compared to healthy patients, the patients with periodontal disease had elevated serum MMP-3, -8, and -9. PD and gingival bleeding decreased along the third week during the 6-week program, but no significant change was found during the sixth week; there were no significant decreases observed during the sixth week. In the periodontal disease group, significant decreases in the concentrations of salivary MMP-3, 8-, and -9 were also found at week 3. Conclusion The present study found that NSPT resulted in decreases of salivary MMP-3, -8, and -9, as well as a nephropathic disease diagnosis, as well as the possibility of MMP-3 as a biomarker.

Source link: https://doi.org/10.1186/s12903-022-02185-3


Immunoregulation and anti-metalloproteinase bioactive injectable polysalicylate matrixgel for efficiently treating osteoarthritis

This work, based on the FPSOH matrixgel with robust anti-inflammatory capability, inhibiting the oxidative stress and nuclear factor kappa B signaling, preventing the metalloproteinase, as well as inducing M2 polarization of macrophages, thus reducing synovial macrophage formation and osteoarthritis progression. In vivo experiments showed that FPSOH hydrogel can prevent papain-induced osteoarthritis and its progression, as well as dual protection for cartilage and synovium.

Source link: https://doi.org/10.1016/j.mtbio.2022.100277


Matrix metalloproteinase 9 gene promoter region -1562 C/T single nucleotide polymorphism increases the susceptibility to hypertensive disorders of pregnancy: a meta-analysis

In the matrix metalloproteinase 9 genes, single nucleotide polymorphisms are common. Both patients and controls were randomly selected with either case-control or cohort studies involving subtypes of HDP and the expression of genotypes and/or alleles within MMP9-1562 C/T. With pooled OR 1. 50 and 1. 36, researchers reported that a variant genotype and allele of MMP9-1562 C/T raised the risk of HDP. In the fixed-effects models, the variant genotype and allele of MMP9-1562 C/T raised risk of preeclampsia and gestational hypertension, according to Subgroup reports.

Source link: https://doi.org/10.31083/j.ceog.2021.02.2265


Sulforaphane Suppresses the Nicotine-Induced Expression of the Matrix Metalloproteinase-9 via Inhibiting ROS-Mediated AP-1 and NF-κB Signaling in Human Gastric Cancer Cells

However, the role of sulforaphane in MMP-9 expression in gastric cancer is yet to be established. MMP-9 production in human gastric cancer cells is minimized by sulforaphane, according to this study. MMP-9 expression in nicotine-induced MMP-9 expression is mediated by reactive oxygen species and MAPKs, according to our researchers. Sulforaphane suppresses nicotine-induced MMP-9 by inhibiting ROS-mediated MAPK/AP-1 and ROS-mediated NF-B signaling axes, which in turn reduce cell proliferation in human gastric cancer AGS cells. As a new anti-invasion tactic for human gastric cancer therapy, the new report found encouraging evidence for the development of sulforaphane.

Source link: https://doi.org/10.3390/ijms23095172


Low Concentration of the Neutrophil Proteases Cathepsin G, Cathepsin B, Proteinase-3 and Metalloproteinase-9 Induce Biofilm Formation in Non-Biofilm-Forming Staphylococcus epidermidis Isolates

Cathepsin G, cathepsin B, proteinase-3, and neutrophil metalloproteinase-9 from neutrophils were tested on the biofilm induction of commensal and clinical non-biofilm-forming S. epidermidis isolates. Proteinase-3 is involved in the biofilm formation kinetics study, according to the addition of phenylmethylsulfonyl fluoride showed that proteinase-3 appears in the cell aggregation stage of biofilm production. A catheter treated with a commensal non-biofilm-producing S. epidermidis isolate treated with proteinase-3 and another without the enzyme were introduced into a mouse's back of a mouse. The catheters were found and the number of grown bacteria was determined in the catheter after 7 days of incubation period, with a higher number of adhered proteinase-3-treated bacteria in the catheter compared to non-proteinase-3-treated bacteria. When multiplicity of infection 1:0. 01 was used, Commensal non-biofilm-forming S. epidermidis in the presence of neutrophil cells significantly promoted biofilm formation, but a mixture of protease inhibitors inhibited biofilm formation.

Source link: https://doi.org/10.3390/ijms23094992


A Disintegrin and Metalloproteinase (ADAM) Family—Novel Biomarkers of Selected Gastrointestinal (GI) Malignancies?

The global burden of gastrointestinal cancers is expected to rise. The expression of several ADAMs is upregulated in GI cancer cells, according to new studies. Hence, the aim of this study is to present current findings regarding ADAMs' role in the development and propagation of colorectal, pancreatic, and gastric cancer. In addition, the prognostic value of selected ADAMs in patients with GI tumours is also discussed. This report, in conclusion, confirms the role of selected ADAMs in the pathogenesis of the most common GI cancers as potential prognostic biomarkers and therapeutic targets for GI malignancies. However, future studies into ADAM biology and treatment of patients with these disorders should be carried out to develop new treatment methods for the diagnosis of these common and fatal malignancies as well as patient care.

Source link: https://doi.org/10.3390/cancers14092307


Atorvastatin regulates the migration and invasion of prostate cancer through the epithelial-mesenchymal transformation and matrix metalloproteinase pathways

Materials and Methods: To determine the effect of ATO on PC3 cell proliferation, we used cell counting kit-8 and clone generation experiments. A mouse xenograft tumor model was developed, and tumor volume and weight were determined. ATO inhibited PC-3 cell proliferation and promoted cell apoptosis in a dose-dependent manner. ATO inhibited invasion and metastasis in PC-3 cells, owing to ATO's ability to reduce the EMT and the expression of MMPs in PC-3 cells, which may have resulted in ATO's inhibition of invasion and metastasis in PC-3 cells. ATO significantly reduced tumor volume and weight in nude mice's studies, according to studies in nude mice; the expression of related proteins was similar to that obtained in vitro results. Conclusions: ATO prevents the occurrence and growth of PCa and regulates the migration and invasion of PCa cells by restricting the EMT and MMPs.

Source link: https://doi.org/10.4111/icu.20210411

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions