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Metagenomics - Springer Nature

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Last Updated: 23 April 2022

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Evaluation of phoxim toxicity on aquatic and zebrafish intestinal microbiota by metagenomics and 16S rRNA gene sequencing analysis

Phoxim is one of the most common organophosphorus pesticides used in agricultural production. The composition of aquatic microbial community changed dramatically when exposed microorganisms, obliterating microorganism interactions, and therefore reduced the variability and stability of the microbial community seven days of phoxim exposure. The zebrafish gut microbial community has also recovered from a temporary ecological imbalance of aquatic microbiota, but phoxim has continued to influence zebrafish growth and swimming habits. Overall, our findings showed that phoxim exposure significantly changed the microbial community's structure and function, as well as a detrimental effect on freshwater ecosystems in a short exposure time.

Source link: https://doi.org/10.1007/s11356-022-20325-8


Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics

The gut microbes play a key role in human longevity, and centenarians' gut microbiota profile reveals some peculiar characteristics from young adults. Most microbial studies on longevity are now based on metagenomic sequencing, which may lead to inaccurate information about the functional microbes that are in such low numbers. Here, we used in-depth metagenomic sequencing and large-scale culturomics to reveal a Chinese longevity population's unique gut microbial community, as well as the potential association between intestinal microbes and longevity. From an individual fecal sample, an average of 51. 1 Gb raw sequencing results were found. The elderly cohort in Hainan province had significantly reduced abundance of butyrate-producing bacteria, as well as a greater number of Escherichia coli, Desulfovibrio piger, and Methanobacter smithii. The unique enriched gut microbes in Hainan's extreme decades population may help to maintain health and longevity.

Source link: https://doi.org/10.1038/s41522-022-00282-3


Diagnosis of Coxiella burnetii infection via metagenomic next-generation sequencing: a case report

However, only a few instances of Coxiella burnetii-caused wound infection have been reported, but the pathogen is very difficult to isolate using traditional culture techniques. Case study A 76-year-old man, underwent endovascular repair of ruptured left iliac aneurysm plus abdominal aortic aneurysm under general anesthesia in 2018. In 2020, left iliac fossa mass resection was performed. Coxiella burnetii was found on the wound tissue for the Metagenomics next-generation sequencing, but Metagenomics next-generation sequencing was performed.

Source link: https://doi.org/10.1186/s12879-022-07309-2


Metagenomic insights into the microbial community structure and resistomes of a tropical agricultural soil persistently inundated with pesticide and animal manure use

Putative ORFs were deciphered by microbiome, heavy metal, and antibiotic resistance of a pesticide and animal manure inundated agricultural soil obtained from a vegetable farm at Otte, Eiyenkorin, N. . . . ia's Kwara State, N. . . . ia, which was deciphered by shotgun metagenomics and functional annotation of putative ORFs. Branches of SL6 microbiome revealed 29 phyla, 49 classes, 924 genera, 424 species, and 260 strains with the preponderance of the phyla Proteobacteria and Actinobacteria, with the genus of Actinobacteria and Actinobacteria, and Gramproteobacteria, respectively. Among other items, aRG analysis using the Antibiotic Resistance Gene-annotation revealed ARGs for 11 antibiotic classes, including a determinant of -lactamases, mobilized colistin resistance determinant, macrolide-streptogramin, glycopeptide, and aminoglycoside resistance genes. According to this report, agricultural soils flooded with pesticide and animal manure are potential hotspots for ARG dissemination and may contribute to the dissemination of multidrug-resistant pathogens.

Source link: https://doi.org/10.1007/s12223-022-00970-9

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions

* Please keep in mind that all text is summarized by machine, we do not bear any responsibility, and you should always check original source before taking any actions