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Microplastic exposure in Daphnia magna may have various toxic effects on the metabolic processes in D. magna; however, the effects of microplastic exposure on D. magna's metabolic pathways and the associated molecular toxicity mechanisms remain unclear; The effects of acute exposure to polyethylene microplastics with various particle sizes on metabolites in D. magna and the mechanisms of toxicity were investigated by combining metabolomics and traditional toxicology methods. Exposure to both MPs-20 and MPs-30 resulted in significant microplastic accumulation in D. magna's gut, which has significantly reduced D. magna survival and heart rate.
Source link: https://doi.org/10.1016/j.chemosphere.2022.135887
A subset of natural products contributes to injury processes in malignant cells that recruit and activate host immune cells in order to produce an adaptive anti-cancer immune response, a process that is also described as immunogenic cell death. Addressing this with a single-cell chemical biology natural product discovery platform, such as multiplex activity metabolomics, can be especially useful in human leukemia, where cancer cells are heterogeneous and react differently to the same substances. Herein, a new ten-color, fluorescent cell barcoding-compatible module measuring six immunogenic cell injury signaling readouts: DNA damage response, apoptosis, necroptosis, mitosis, autophagy, and unfolded protein reaction reveals the unfolded protein response. Next, the immunogenic effects of injury pathways on immunogenicity were compared to three canonical assays for immunogenic hallmarks, ATP, HMGB1, and calreticulin, to determine secondary metabolite-induced cell death profiles with canonical markers of immunogenic cell death.
Source link: https://doi.org/10.1016/j.jbc.2022.102300
Metabolomics is a highly effective strategy for this task, because it involves the analysis of a set of metabolites present in a biological system undergoing a biological process, such as a disease or cancer. In metabolomics, state-of-the-art analytical methods are used to identify and quantify the metabolites present in the tested biological samples, as well as chemical and bioinformatic techniques that can reveal the specific metabolites and metabolic pathways that are relevant to the biological process under investigation.
Source link: https://doi.org/10.1007/978-3-030-97182-3_8
Metabolic reprogramming plays a vital role in prostate cancer formation. However, there are no studies on the effects of nanomaterials as vectors on cancer metabolism reprogramming. Using salt-aging conjugation techniques, Herein, a form of nanoparticle with good biocompatibility, was synthesized by changing the double-stranded DNA containing a sulfhydryl group on the surface of gold nanoparticles by altering the double-stranded of DNA containing a sulfhydryl group. In vitro, the resulting AuNPs-dsDNA complexes had low toxicity to PC3 and DU145 cells. Both in PC3 and DU145 cells, assay by Seahorse assay showed that AuNPs-dsDNA complexes could increase glycolysis and glycolysis capacity. In addition, untargeted metabolomics revealed that PC and PC levels had been decreased and the inosinic acid level in PC3 cells had been raised. In summary, we found that AuNPs and AuNPs-dsDNA complexes may play a role in cancer cell metabolism reprogramming largely by the lipid metabolic pathways, which may account for the previously mentioned phenomenon of enhanced glycolysis and glycolysis capacity.
Source link: https://doi.org/10.1016/j.bioadv.2022.212745
In animal models, CR had been shown to reduce inflammation and exacerbate Gastric Precancerous Lesions. Given the combination of proteomics and metabolomics, we found that CR could significantly reduce the biological pathways relating to energy metabolism that had been affected by the Gastric Precancerous Lesions model's limitations. In addition, the results of serum pharmacology revealed that the Codonopsis Radix-containing serum could prevent gastritis injury and selectively restrict the proliferation of gastric cancer cells rather than normal cells, which was closely related to ATP production in the above mentioned cells. Conclusion: In summary, CR had anti-Gastric Precancerous Lesions by reducing gastritis injury and selectively limiting the proliferation of gastric cancer cells rather than normal cells. This report not only provided new insights into the behaviour of intricate systems such as Chinese herbals, but also enhanced Gastric Precancerous Lesions' treatment program by regulating energy metabolism.
Source link: https://doi.org/10.3389/fphar.2022.933096
History: Prepared rhubarb was obtained by steaming raw rhubarb with wine. Prepared rhubarb has the ability of increasing blood circulation and removing blood stasis, which is different from raw rhubarb with a purgative effect. Purpose: The aim of this research was to investigate active chemical components in pre-prepared rhubarb's occurrence of noxious heat blood stasis syndrome by extensive metabolomics profiling. The metabolomics profiles of rat plasma from model and control groups were dramatically different, with 31 endogenous metabolites modified by NHBS. A total of 28 chemicals from prepared rhubarb absorption into the plasma were discovered, including nine prototypes and 19 metabolites. According to statistical analysis, rhein and its metabolites account for half of the top ten active compounds in a compiled rhubarb for its biomedical uses. Conclusion: In the way of metabolomics, this research provided evidence for the therapeutic effects and active chemicals of pre-prepared rhubarb's NHBS.
Source link: https://doi.org/10.3389/fphar.2022.907831
Psoriasis vulgaris is a complicated autoimmune disease characterized by erythema of the skin and a lack of readily available cures. Aim: Psoriasis vulgaris is a complex autoimmune disease characterized by erythema of the skin and a lack of readily available cures. Patients with PV were recruited at a dermatology clinic and classified according to the presence of metabolic comorbidities and their Psoriasis Area and Severity Index from January 2019 to November 2019. We conducted targeted metabolomic tests of serum samples and established the correlation between metabolite composition and PASI scores, which revealed the connection between metabolite composition and PASI scores. PV patients were referred to a 'PVM group,'"PV group, or a "PV group") and then subdivided into a "mild PV" and a "severe PV" group, and a "severe PV" group. Compared to the matched healthy controls, the MP subgroup's characteristics, as well as 28 metabolites in the SP subgroup, were found to have been altered. SM and SM were positively related to the PASI in the MP subgroup, while Cer, PC, and PC were positively correlated with the PASI in the SP subgroup, among other items. Compared to matched patients from the PV group, PVM's levels of 17 metabolites, particularly ceramides and phosphatidylcholine, were elevated, particularly ceramides and phosphatidylcholine. In addition, the correlation results showed that Cer and SM were not only linked to PASI but also has strong correlations with biochemical results. Conclusion: The findings of this research reveal that patients with PV at various severity levels have varying metabolic profiles, and that metabolic disorders can delay disease development.
Source link: https://doi.org/10.3389/fmolb.2022.945917
In utero on prenatal ultrasonography, Renal pelvis dilatation is identified and can resolve spontaneously. The diagnosis of UPJO can only be confirmed after birth with repeat U. S. and renal isotope studies. To determine specific urinary biomarkers for UPJO, specific urinary biomarkers were identified for UPJO, the nuclear Magnetic Resonance spectroscopy was performed on urine of newborns with prenatally diagnosed unilateral RPD and healthy controls. Patients with UPJO i. e. Two key metabolic pathways appeared to be impaired in patients with UPJO I. e. Patients with transient dilatations and controls are specifically identified patients who require surgery for UPJO, irrespective of patients with transient dilatations and controls in this prospective review.
Source link: https://doi.org/10.1038/s41598-022-17664-4
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