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In a group of healthy women, recent metabolomic results showed that the temporal abundance data of metabolome can be used to predict delivery timing with high accuracy. In asymptomatic women of singleton pregnancies, method LC-MS/MS quantitative assays were used to determine the plasma concentrations of four steroid metabolites, including oestriol-16-glucuronide, 17-alpha-hydroxyprogesterone, tetrahydrodeoxycorticosterone, tetrahydrodeoxycorticosterone te a ton pregnasymptomatic women of singleton pregnancies asymptomatic women of asymptomatic women of asymptomatic women of four metaboliton metabolites, including metaboliton metabolites, 17-ton hydroxyprogenide, 17-tedoe hydroxyprogesterone, te tete, tanthotetetetanodeoxycortanthrothrothronodeoxycorticodeoxycorticodeoxycorticodeoxycorticodeoxycor The coefficients of variation of the four analytes at the lower limit of quantification varied from 8. 9% to 14. 6%, with R2 values higher than 0. 990 in the calibration curves. According to the experts, 585 recruited pregnant women who ended up with spontaneous delivery, 17. 1% and 82. 9% of the women were delivered within and after 7 days after plasma collection, respectively. The gestational age-adjusted area under the curve of the combined measurements of E3-16-Gluc and 17-OHP was 0. 69 %, with the sensitivity of 87. 0% and specificity of 60. 2%. Conclusion We performed quantitative and clinical testing of a quantitative LC-MS/MS panel for the four steroids in pregnant women's plasma. E3-16-Gluc and 17-OHP's steroid metabolites panel was potentially useful in predicting delivery within one week in asymptomatic women of singleton pregnancies. At the time of sampling in women who received within one week, the levels of E3-16-Gluc and 17-OHP were found to be significantly elevated, and their combined testing may be highly useful in delivery prediction.
Source link: https://europepmc.org/article/MED/35467464
The aim of this research was to create a validated liquid chromatography-tandem mass spectrometry system for the analysis of atorvastatin, rosuvastatin, and their key metabolites, as well as investigating the effect of statin therapy on various hematological and biochemical variables. In a total of 210 patients with unstable angina pectoris, ST-elevation myocardial infarction, or non-ST-elevation myocardial infarction, the amounts of drugs and their metabolites were determined by the validated method. It was reported that six months of statin therapy had minor changes in biochemical and hematological parameters.
Source link: https://europepmc.org/article/MED/35487588
Organophosphate esters and their diester metabolites have been found in a variety of environmental matrices and classified as emerging environmental pollutants, although data on their presence in food and human matrices is still limited. In this research, a novel and simple technique was used to simultaneously measure 14 OPEs and 6 diester metabolites in dairy products and human milk. A frozen-dried milk sample was extracted with acetonitrile and purified by solid-phase extraction after enzymatic hydrolysis by -glucuronidase/arylsulfatase. Most analytes had signal suppression, according to a test for matrix effects, and isotopically labeled ISs were useful for compensating for matrix effects. In actual samples, OPEs and their metabolites showed high detection frequencies, suggesting that these new chemicals were ubiquitous in food and the human body, and that the effect of the diester metabolites on population exposure must be factored in exposure analysis.
Source link: https://europepmc.org/article/MED/35449470
Organophosphate flame retardants and organophosphate pesticides, which refer to organophosphate esters, are ubiquitous in the environment and have been shown to pose significant risks to human health. For the simultaneous analysis of multiple organophosphorus metabolites in urine, a fast and sensitive method based on a solid phase extraction and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry analysis has been used to evaluate human exposures to OPFRs and OPPs. The excellent results and reliability of our method's determination and stability in a cross-validation test with a new gas chromatography-mass spectrometry device and a good inter-laboratory deal for several OPFR metabolites in a common reference material reinforced our method's consistency and reliability. In 35 Chinese children's urine samples, the established method was used to analyze 16 organophosphorus metabolites.
Source link: https://europepmc.org/article/MED/35427657
The conditionally essential amino acid arginine and its metabolic products play a significant role in various biological processes, such as immune response control, macrophage activation and polarization, and T cell function control. Similarly, altered polyamine metabolism may be correlated with neurodegenerative disorders, while polyamine levels can be used to determine Parkinson's disease severity or non-alcoholic fatty liver disease progression. Cell lysates, cell culture supernatant, and tissue extracts were found at 103. 2%, 99. 0%, 4. 3 percent, and 93 percent 4. 3%, respectively. Cell lysates, 6. 7 to 14. 8% in cell culture supernatants, and 5. 3 to 12. 0% in tissue extracts were all between 5. 9 and 14. 8%.
Source link: https://europepmc.org/article/MED/35421734
There are studies focusing on the clinical drug testing of second and third-generation epidermal growth factor receptor-tyrosine kinase inhibitors in non-small cell lung cancer patients and are limited, and they must improve the effectiveness of EGFR-TKI therapy. Some EGFR-TKIs have active metabolites with similar or higher potency to the parent compounds; therefore, monitoring the parent compound as well as the active metabolites is essential for truly effective TDM. We developed and validated a method that simultaneously measures second- and third-generation EGFR-TKIs and the active metabolites of osimertinib, AZ5104 and AZ7550 in human serum by liquid chromatography-tandem mass spectrometry in the human serum. Excellent linearity of calibration curves was observed at ranges of 2. 5-125. 0 ng/mL for afatinib, 2. 5-125. 0 ng/mL for dacomitinib, 4. 0-800. 0 ng/mL for osimertinib, 1. 0-125. 0 ng/mL for AZ5104, 2. 5-125. 0 ng/mL for osimertinib, 1. 0-125. 0 ng/mL for ng/mL for mL for mL for mL for mL for ng/mL for mL for mL for mL for mL for mL for mL for dacomitinib, mL for dacomitinib, mL for mL for dacomitinib, mL for mL for mL for dacomitinib, 0. 05 mL for dacomitinib, mL for dacomitinib, 0. 90 1. 0 1. 0 4. 0 mL.
Source link: https://europepmc.org/article/MED/35436724
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